2000
DOI: 10.1292/jvms.62.207
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Sandwich Enzyme-Linked Immunosorbent Assay of Canine Leptin.

Abstract: ABSTRACT. Leptin is the ob gene product secreted from adipocytes in mammals, and thereby its plasma level reflects body fat content. To establish an assay method for leptin in the dog, rabbit anti-canine leptin antibody was obtained using canine recombinant leptin as an antigen. This antibody reacted to canine leptin much stronger than mouse, rat and human leptins. Sandwich enzyme-linked immunosorbent assay (ELISA) using this antibody was developed. The serum leptin levels of 13 healthy dogs were in a range fr… Show more

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Cited by 29 publications
(25 citation statements)
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“…Although these low values may be a consequence of an inability of the kit to detect seal leptin rather than a reflection of true concentrations, circulating leptin concentrations in other carnivores are low (20). In seals, high assay variability combined with low actual leptin concentrations may mask important patterns of change in leptin secretion.…”
Section: Discussionmentioning
confidence: 99%
“…Although these low values may be a consequence of an inability of the kit to detect seal leptin rather than a reflection of true concentrations, circulating leptin concentrations in other carnivores are low (20). In seals, high assay variability combined with low actual leptin concentrations may mask important patterns of change in leptin secretion.…”
Section: Discussionmentioning
confidence: 99%
“…Since plasma leptin level is closely related to metabolic condition, it can be a strong tool for the study of obesity and related disorders. Previously, we cloned canine leptin gene (Iwase et al, 2000b), produced recombinant protein, and established a sandwich ELISA specific to canine leptin (Iwase et al, 2000a). Using this ELISA method, we demonstrated a highly positive correlation between plasma leptin concentration and body fat content estimated by a deuterium oxide dilution method in experimentally induced obese beagles (Sagawa et al, 2002, Ishioka et al, 2002b.…”
Section: Introductionmentioning
confidence: 93%
“…The plasma leptin concentration was measured by the previously reported method of sandwich ELISA using an anti-canine leptin antibody (Iwase et al, 2000a) with minor modifications. Briefly, each well of a 96-well microplate was coated with 0.2 µg of the purified rabbit anti-canine leptin antibody and incubated for 2 hr at room temperature.…”
Section: Leptin Assaymentioning
confidence: 99%
“…The plasma supernatant was transferred to 1.5-ml microfuge tubes and stored at Ϫ80°C until analyses were performed. Plasma leptin was measured with a sandwich ELISA for canine leptin as previously described (11). Briefly, rabbit anti-canine leptin antibodies were produced using canine recombinant leptin as an antigen.…”
mentioning
confidence: 99%