San-Huang-Xie-Xin-Tang protects cardiomyocytes against hypoxia/reoxygenation injury via inhibition of oxidative stress-induced apoptosis

Liou, Shu Fen; Hsu, Jong Hau; Liang, Jyh Chong; Ke, Hung Jen; Chen, Ing Jun; Wu, Jiunn Ren; Yeh, Jwu‐Lai

doi:10.1007/s11418-011-0592-0

Cited by 42 publications

(31 citation statements)

References 31 publications

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“…Some signaling cascades such as phosphatidylinositol-3-kinase (PI3K) and serine/threonine kinase (Akt) pathways may inhibit CM hypertrophy [7], [8]. The endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) pathway, known as an important factor in regulating vascular function and one of the down-stream of Akt signaling, has also been shown to reduce ROS generation and exert anti-apoptotic effect on CMs [9], [10].…”

Section: Introductionmentioning

confidence: 99%

EPC-Derived Microvesicles Protect Cardiomyocytes from Ang II-Induced Hypertrophy and Apoptosis

Zhang

Chen

et al. 2014

PLoS ONE

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Cell-released microvesicles (MVs) represent a novel way of cell-to-cell communication. Previous evidence indicates that endothelial progenitor cells (EPCs)-derived MVs can modulate endothelial cell survival and proliferation. In this study, we evaluated whether EPC-MVs protect cardiomyocytes (CMs) against angiotensin II (Ang II)-induced hypertrophy and apoptosis. The H9c2 CMs were exposed to Ang II in the presence or absence of EPC-MVs. Cell viability, apoptosis, surface area and β-myosin heavy chain (β-MHC) expression were analyzed. Meanwhile, reactive oxygen species (ROS), serine/threonine kinase (Akt), endothelial nitric oxide synthase (eNOS), and their phosphorylated proteins (p-Akt, p-eNOS) were measured. Phosphatidylinositol-3-kinase (PI3K) and NOS inhibitors were used for pathway verification. The role of MV-carried RNAs in mediating these effects was also explored. Results showed 1) EPC-MVs were able to protect CMs against Ang II-induced changes in cell viability, apoptosis, surface area, β-MHC expression and ROS over-production; 2) The effects were accompanied with the up-regulation of Akt/p-Akt and its downstream eNOS/p-eNOS, and were abolished by PI3K inhibition or partially blocked by NOS inhibition; 3) Depletion of RNAs from EPC-MVs partially or totally eliminated the effects of EPC-MVs. Our data indicate that EPC-MVs protect CMs from hypertrophy and apoptosis through activating the PI3K/Akt/eNOS pathway via the RNAs carried by EPC-MVs.

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Section: Introductionmentioning

confidence: 99%

EPC-Derived Microvesicles Protect Cardiomyocytes from Ang II-Induced Hypertrophy and Apoptosis

Zhang

Chen

et al. 2014

PLoS ONE

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“…It has been reported that this compound exhibits many different pharmacological activities. Baicalin has displayed beneficial effects on several diseases model such as hypoxia/reoxygenation caused cardiomyocytes injury [9], hepatic cytotoxicity [10], iron-overloaded mouse [11], rheumatoid arthritis [12] and so on. Furthermore, recent studies have confirmed the photoprotective effect of baicalin against acute and multiple UVB-induced photodamage [13], [14], [15], [16], and this effect is thought to be associated with reduction of oxidative stress [17].…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study

Zhang

Yin

et al. 2014

PLoS ONE

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ObjectiveThis study was aimed to evaluate the anti-photoaging effects of baicalin on Ultraviolet B (UVB)-induced photoaging in the dorsal skin of hairless mice and premature senescence in human dermal fibroblasts.MethodsWe established in vivo and in vitro photoaging models by repeated exposures to UVB irradiation. By HE staining, masson staining, immunohistostaing and real-time RT-PCR, we analyzed epidermal thickness, collagen expression and the mRNA and protein levels of type I collagen, type III collagen, interstitial collagenase (MMP-1 and MMP-3) in UVB exposed dorsal mice skin. The aging condition in human dermal fibroblasts was determined by senescence-associated β-galactosidase (SA-β-gal) staining. Cell viability was determined using the Cell Counting Kit-8 (CCK-8). The G1 phase cell growth arrest was analyzed by flow cytometry. The senescence-related protein levels of p16INK-4a, p21WAF-1, and p53 and protein levels of phosphorylated histone H2AX were estimated by Western blotting.ResultsTopically application of baicalin treatment reduced UVB-induced epidermal thickening of mouse skin and also result in an increase in the production of collagen I and III, and a decrease in the expression of MMP-1 and MMP-3. Compared with the UVB-irradiated group, we found that the irradiated fibroblasts additionally treated with baicalin demonstrated a decrease in the expression of SA-β-gal, a increase in the cell viability, a decrease in the G1 phase cell proportion, a downregulation in the level of senescence-associated and γ-H2AX proteins. However, Baicalin had no difference in the normal fibroblasts without UVB irradiation and long-term Baicalin incubation of UVB-SIPS fibroblasts gave no effects on the cell proliferation.ConclusionsTaken together, these results suggest that baicalin significantly antagonizes photoaging induced by UVB in vivo and in vitro, indicating the potential of baicalin application for anti-photoaging treatment.

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“…According to the literature, SHSST has been used in East Asia as a representative herbal medicine to treat both hypertension and its complications (Hur, 1613). Recently, scientific studies have newly demonstrated that SHSST has pharmacological efficacy, due to its having anti-inflammatory, anti-oxidative stress, anti-cancer, and neuroprotective properties (Li et al, 2010; Shih et al, 2011; Liou et al, 2012; Lo et al, 2012). These properties would mainly be associated with the constituents of SHSST such as berberine, coptisine, berberastine, worenine, and palmatine from Coptis Rhizome (Manisha, 2009); hydroxyanthracene derivatives (emodin, physcione, aloe-emodin, and chrysophanol glycosides) from Rhei Rhizoma (Manisha, 2009); and aglycones (baicalein, wogonin, oroxylin A) and glycosides (baicalin, wogonoside, oroxylin A-7-glucuronide) from Scutellariae Radix (Li et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…SHSST has traditionally been used in Korea, China, and Japan as a curative for both hypertension and its complications; and it is still widely used for both purposes (Hur, 1613; Jin et al, 2007). According to previous laboratory and clinical studies, SHSST plays a role in the treatment of various diseases including hypertension (Iijima et al, 2000; Lo et al, 2005b; Jin et al, 2007), cardiac disorder (Liou et al, 2012), gastric inflammatory symptoms (Shih et al, 2007), acute lung injury (Lo et al, 2005a), and hepatic disorder (Huang et al, 2011). SHSST also exerts a neuroprotective effect in 6-OHDA-induced toxicity in neuronal SH-SY5Y cells (Shih et al, 2011) and in the MPP(+)/MPTP-induced toxicity, in vitro and in vivo model for Parkinson’s disease (Lo et al, 2012) via its anti-inflammatory, anti-oxidative, and anti-apoptotic activities.…”

Section: Introductionmentioning

confidence: 99%

Oriental Medicine Samhwangsasim-tang Alleviates Experimental Autoimmune Encephalomyelitis by Suppressing Th1 Cell Responses and Upregulating Treg Cell Responses

et al. 2017

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Oriental medicine Samhwangsasim-tang (SHSST) has traditionally been used in East Asia to treat hypertension and its complications. However, little is known about its potential value regarding the treatment of chronic inflammatory diseases such as multiple sclerosis (MS). In this study, we investigated whether SHSST has a beneficial effect in treating myelin oligodendrocyte glycoprotein-induced experimental autoimmune encephalomyelitis (EAE). Onset-treatment with SHSST was found to alleviate neurological symptoms as well as demyelination and glial activation in the spinal cords from the EAE mice. The SHSST also attenuated the mRNA or protein expression of pro-inflammatory cytokines (interleukin-1beta and tumor necrotic factor-alpha); chemokines (RANTES, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha); inducible nitric oxide synthase; and cyclooxygenase-2 in correspondence with the down-regulation of the nuclear factor-kappa B and mitogen-activated protein kinases signal pathways in the spinal cords from EAE mice. Interestingly, the protective effect of the SHSST was related to a decreased number of Th1 cells and an increased number of Treg cells in spinal cords from EAE mice. Taken together, our finding firstly suggested that SHSST could delay or mitigate EAE with a wide therapeutic time-window by suppressing Th1 cell responses and upregulating Treg cell responses. Also, our findings are strong enough to warrant further investigation of SHSST as a treatment for chronic autoimmune diseases including MS.

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San-Huang-Xie-Xin-Tang protects cardiomyocytes against hypoxia/reoxygenation injury via inhibition of oxidative stress-induced apoptosis

Cited by 42 publications

References 31 publications

EPC-Derived Microvesicles Protect Cardiomyocytes from Ang II-Induced Hypertrophy and Apoptosis

EPC-Derived Microvesicles Protect Cardiomyocytes from Ang II-Induced Hypertrophy and Apoptosis

The Protective Effect of Baicalin against UVB Irradiation Induced Photoaging: An In Vitro and In Vivo Study

Oriental Medicine Samhwangsasim-tang Alleviates Experimental Autoimmune Encephalomyelitis by Suppressing Th1 Cell Responses and Upregulating Treg Cell Responses

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