Sampling time after tourniquet removal affects erythrocyte deformability and aggregation measurements

Connes, Philippe; Üyüklü, Mehmet; Tripette, Julien; Boucher, John H.; Beltan, Eric; Chalabi, Tawfik; Yalçın, Özlem; Chout, R.; Hue, Olivier; Hardy‐Dessources, Marie‐Dominique; Başkurt, Oğuz K.

doi:10.3233/ch-2009-1146

Cited by 9 publications

(6 citation statements)

References 6 publications

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“…20,21 In addition, recent work has demonstrated its importance for the understanding of vascular disturbances in obese patients 22 and after removing tourniquets. 23 In vascular diseases such as diabetes and hypertension, the shape of rouleaux is altered, producing large globular aggregates that can lead to a blockage in microcirculation. 14,24 These obstructions reduce the disintegrating forces, resulting in an incremental increase in the size of the aggregates, consequently disturbing the mentioned flow and causing a feedback situation, which leads to an escalation of blood viscosity.…”

Section: Red Blood Cell Aggregation In Pathologiesmentioning

confidence: 99%

Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip

2017

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The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in the ligands present in plasma. The process of erythrocyte aggregation was studied in stasis situation (free shear stresses), using an optical chip based on the laser transmission technique. Kinetic curves of erythrocyte aggregation under different conditions were obtained, allowing evaluation and characterization of this process. Two main characteristics of blood that influence erythrocyte aggregation were analyzed: the erythrocyte surface anionic charge (EAC) after digestion with the enzyme trypsin and plasmatic protein concentration in suspension medium using plasma dissolutions in physiological saline with human albumin. A theoretical approach was evaluated to obtain aggregation and disaggregation ratios by syllectograms data fitting. Sensible parameters ( Amp 100 , t 1 \ 2 ) regarding a reduced erythrocyte EAC were determined, and other parameters (AI, M-Index) resulted that are representative of a variation in the plasmatic protein content of the suspension medium. These results are very useful for further applications in biomedicine.

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Section: Red Blood Cell Aggregation In Pathologiesmentioning

confidence: 99%

Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip

2017

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“…Table 1 shows the total blood collection time of the robot (204.9 ± 1.9 s) was significantly higher than that of the manual group (51.9 ± 7.1 s). Although the tourniquet lasted less than 1 min, the arm fixation belt also exerts a certain pressure on blood vessels when the robot collects blood, and the duration is longer, which may have an impact on the detection results ( 5 , 6 ). The blood collection settings need further improvement.…”

Section: Discussionmentioning

confidence: 99%

Clinical application of a fully automated blood collection robot and its assessment of blood collection quality of anticoagulant specimens

Wang,

Gu,

Zhu

et al. 2023

Front. Med.

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Background and objectivesTo investigate the application of intelligent puncture blood collection robots in anticoagulated blood specimens, the satisfaction of subjects with the two blood collection methods, and the feasibility of intelligent blood collection devices to replace manual blood collection methods in clinical work.Materials and methodsA total of 154 volunteers from Zhongshan Hospital Fudan University were recruited to compare the test results of anticoagulant blood samples between blood collection robot and manual blood collection, a questionnaire was used to inquire about the volunteers’ feelings about the two blood collection methods; the blood collection data of 6,255 patients willing to use the robot for blood collection were collected to analyze the success rate of blood collection.ResultsThe blood collection robot is superior to manual specimen collection in terms of volume and pain of specimen collection, and the puncture success rate is 94.3%. The anticoagulated blood specimens collected by the robot had 11 indexes statistically different from the results of manual blood collection, but the differences did not affect the clinical diagnosis and prognosis.ConclusionThe intelligent robotic blood collection is less painful and has better acceptance by patients, which can be used for clinical anticoagulated blood specimen collection.

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“…Current evidence suggests that prolonged tourniquet application time (> 1 min) can lead to results exceeding the current analytical quality specifications for desirable bias, either in biochemical (total protein, albumin, potassium and calcium) or heamathological testing (WBC, RBC, hemoglobin, hematocrit, lymphocytes and monocytes) [5,6,12,13]. Interestingly, platelet function assessed by multiple electrode aggregometry [3], and erythrocyte deformability and aggregation also seem to be significantly affected [14,15]. Higher rates of hemolyzed samples are also associated with tourniquet application times greater than one minute [7,8], leading to rejection and new sample request, impacting on tests turnaround times.…”

Section: Discussionmentioning

confidence: 99%

Improving the quality of venous blood sampling procedure (phlebotomy): avoiding tourniquet use

Freitas

Alves

2020

Preprint

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Background: Guidelines for venous blood sampling procedure (phlebotomy) discourage tourniquet use whenever possible. Here, we aim to assess Biomedical Scientists capability of not using the tourniquet in phlebotomy, which we hypothesised to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site. Materials and Methods: We selected and assigned two (BMS) with the same age (41 years) and experience (20 years) to record ten phlebotomy days, the first with prioritized and the latter with non-prioritized patients. In a simple record form, each acquired daily data for the number of attended patients, age and gender, the frequency of non-tourniquet usage and the punctured vein. To test our work hypothesis we used the two-tailed single sample t-test (p < 0.05). Differences between age-group means and non-tourniquet use means by each BMS were tested by two-tailed t-test for independent means (p < 0.05). Results: In 10 phlebotomy days 683 patients were attended, with males representing 43,2% of the population. We found no statistically difference between age-group means. The combined capability of non-tourniquet use was 50,5%, which did not differ from our null hypothesis, but the individual group-means were statistically different, being 33% and 66.9% in the prioritized vs non-prioritized group. The medial cubital vein was the most prone to be punctured (77,7%). Conclusions: We have shown that performing phlebotomies without tourniquet use is possible and desirable in at least half of the attended patients, though being more limited in specific group populations. Our results provide room for quality improvement in the laboratory pre-analytical phase.

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Sampling time after tourniquet removal affects erythrocyte deformability and aggregation measurements

Cited by 9 publications

References 6 publications

Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip

Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip

Clinical application of a fully automated blood collection robot and its assessment of blood collection quality of anticoagulant specimens

Improving the quality of venous blood sampling procedure (phlebotomy): avoiding tourniquet use

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