Sampling the Rat Mesenteric Artery

Abstract

Vascular injury can be induced by different classes of drug candidates, and it can affect the mesenteric vasculature. Sampling of the mesenteric vessels in the rat is crucial for proper assessment of potential adverse or pharmacologic effects of drugs in nonclinical rodent studies. To date, several sampling and processing techniques for the histopathologic evaluation of the mesenteric artery in rodents have been described and used in studies with candidate drugs that may affect the vascular system. However, most of those techniques require a significant amount of time and effort. A less labor-intensive, time-consuming, and expensive technique that allows examination of the mesentery vasculature with abundant longitudinal and cross sections of the vessels when examined microscopically was developed and presented here.

Detection of fenoldopam-induced arteritis in rats using ex vivo / in vivo MRI

“…While the mesenteric artery is the predominant site for drug-induced arteritis in rats [17] , it was difficult to obtain a clear image because the mesenteric artery is free to move within the abdominal cavity and is strongly affected by respiratory and peristaltic movements. However, as shown in this report, by using a respiratory gating technique and suppressing peristalsis, arteritis in the mesenteric artery can be detected by MRI.…”

Unaltered Long-Term Maternal Endothelin System in a Gestational Hypertensive Rat Model

“…Subsequently, the mesenteric arteries were harvested as described in the previous study. 25 For the immunohistochemistry study, the samples were fixed with 10% neutral buffered formalin (NBF) for 24 hours and proceeded to make a formalinfixed paraffin-embedded (FFPE) block.…”