Sampling membrane potential, membrane resistance and electrode resistance with a glass electrode impaled into a single cell

Schiebe, M.; Jaeger, Ulrich

doi:10.1016/0165-0270(80)90060-6

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…Because H + ions are involved in this electrochemical response, as per the Nernst equation, the peak potential changed in a wide pH range as expressed by the following Equation (1): In this study, a simple pH sensor was fabricated by immobilizing Azure A (AA) whose oxidation and reduction potentials change depending on the solution pH using a layer-by-layer deposition method. Because pH is an important physical property of aqueous solutions, various methods have been used for pH measurements, such as glass membrane electrodes [35,36], ion-sensitive field effect transistor (ISFET) [37,38], pH indicators, and fluorescent probes [39,40]. The electrochemical method of pH measurement in which measurement of an electric current is involved is characterized by certain advantages, such as low cost, ease of operation, and ease of electrode fabrication, and is useful for developing simple sensors.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric pH Measurements Using Azure A-Containing Layer-by-Layer Film Immobilized Electrodes

et al. 2020

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pH is one of the most important properties associated with an aqueous solution and various pH measurement techniques are available. In this study, Azure A-modified poly(methacrylic acid) (AA-PMA) was synthesized used to prepare a layer-by-layer deposited film with poly(allylamine hydrochloride) (PAH) on a glassy carbon electrode via electrostatic interactions and the multilayer film-immobilized electrode was used to measure pH. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurement were performed. Consequently, the oxidation potential of AA on the electrode changed with pH. As per Nernst’s equation, because H+ ions are involved in the redox reaction, the peak potential shifted depending on the pH of the solution. The peak potential shifts are easier to detect by DPV than CV measurement. Accordingly, using electrochemical responses, the pH was successfully measured in the pH range of 3 to 9, and the electrodes were usable for 50 repeated measurements. Moreover, these electrochemical responses were not affected by interfering substances.

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Section: Introductionmentioning

confidence: 99%

Voltammetric pH Measurements Using Azure A-Containing Layer-by-Layer Film Immobilized Electrodes

et al. 2020

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“…Fig. 1 (16,20). According to Rubio and Zubieta (16), this overshoot can be interpreted as an electroosmotic phenomenon which could confuse the measurements.…”

Section: Methodsmentioning

confidence: 99%

“…Vol. 72, 1983 experiments, however, with the SEC system of Schiebe and Jaiger (20), which is based on the same principles, yielded good results (19). To resolve this obvious contradiction, we have compared our SEC method with the conventional two-electrode-current clamp method introduced by Weidmann (25).…”

Section: Methodsmentioning

confidence: 99%

“…The technique of Schiebe, Jager, and Pauschinger (20,21), which we employed, is novel with regard to the applied pulse program (see below), the data acquisition, and the data processing methods. But it relies in principle on the pioneering work of Schanne and Ceretti (17,18).…”

mentioning

confidence: 99%

“…In very small and/or highly turgescent cells, however, these methods are not applicable. A newly developed SEC5 technique (20,21) experimental conditions. To test the usefulness of this new technique in cell impedance measurements, we experimentally compared this SEC method with the conventional two-electrode method.…”

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confidence: 99%

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Continuous Registration of Membrane Input Resistances of Small Plant Cells Using a Double-Pulse Current Clamp Technique for Single-Electrode Impalements

Schefczik¹,

Simonis²,

Schiebe³

1983

Plant Physiol.

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To measure the cell input resistance in Elo&a leaf cells, a new singlemicroelectrode method was explored by comparing the results with conventional two-microelectrode experiments. The new method takes advantage of the difference in the frequency response curves between electrode and cell impedances. By application of electrical stimuli, which contain specific frequency bands, the different impedances can be analyzed separately. To get a distinct separation in the frequency response of cell and electrode, respectively, the electrode capacitance has to be compensated during the impalement. Dffferent time constants of the cell membrane can be accounted for by adjustment of the stimulus length. It is shown that both the single-and the double-electrode method yield the same results, even Uf the cell input resistances change considerably during the course of the experiment. This demonstrates the usefulness of the new single-electrode method for continuous measurements of cell membrane resistances, especialy in cells so small that the double-electrode method is no longer applicable.Normally, two-electrode methods are used to measure impedances and membrane potentials in single cells (7,25). In very small and/or highly turgescent cells, however, these methods are not applicable. A newly developed SEC5 technique (20, 21), nevertheless, offers reliable results, also for such unfavorable 'Dedicated to the memory of Prof. Dr. Noe Higinbotham.

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Morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus, type 9

1988

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The membrane potential, Em, of human granulocytes (PMNs), was recorded using glass microelectrodes. The membrane potential Em exhibited potential fluctuations accompanied by characteristic changes of cell shape. The periodic potential fluctuations (7-s, 70-s, and 260-s periodicities) ascertained by the autocorrelation technique, suggested the existence of an internal clock. The chemoattractant f-Met-Leu-Phe (FMLP) had no influence on the periodicities, whereas the amplitudes of the fluctuations were increased by it. Treatment of PMNs with Echo 9 virus also resulted in hyperpolarization. The 70-s periodicity disappeared under virus treatment indicating a virus-induced change of the internal program and loss of chemotactic activity.

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Sampling membrane potential, membrane resistance and electrode resistance with a glass electrode impaled into a single cell

Cited by 5 publications

References 7 publications

Voltammetric pH Measurements Using Azure A-Containing Layer-by-Layer Film Immobilized Electrodes

Voltammetric pH Measurements Using Azure A-Containing Layer-by-Layer Film Immobilized Electrodes

Continuous Registration of Membrane Input Resistances of Small Plant Cells Using a Double-Pulse Current Clamp Technique for Single-Electrode Impalements

Morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus, type 9

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