Sampling for malaria molecular surveillance

Mayor, Alfredo; Ishengoma, Deus S.; Proctor, Joshua L.; Verity, Robert

doi:10.1016/j.pt.2023.08.007

Cited by 7 publications

(2 citation statements)

References 77 publications

(114 reference statements)

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“…For continuous genomic surveillance of malaria, samples must be collected regularly, and, especially critical for low-resource settings, cost-efficiently 2 , 13 , 14 . Pregnant women attending their first antenatal care (ANC) consultation are an easy-access subpopulation that could potentially serve as a sentinel group for malaria surveillance 13 , 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

Genomic malaria surveillance of antenatal care users detects reduced transmission following elimination interventions in Mozambique

Brokhattingen,

Matambisso,

da Silva

et al. 2024

Nat Commun

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Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compare the genetic structure of parasite populations sampled from 289 first ANC users and 93 children from the community in Mozambique between 2015 and 2019. Samples are amplicon sequenced targeting 165 microhaplotypes and 15 drug resistance genes. Metrics of genetic diversity and relatedness, as well as the prevalence of drug resistance markers, are consistent between the two populations. In an area targeted for elimination, intra-host genetic diversity declines in both populations (p = 0.002-0.007), while for the ANC population, population genetic diversity is also lower (p = 0.0004), and genetic relatedness between infections is higher (p = 0.002) than control areas, indicating a recent reduction in the parasite population size. These results highlight the added value of genomic surveillance at ANC clinics to inform about changes in transmission beyond epidemiological data.

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Section: Introductionmentioning

confidence: 99%

Genomic malaria surveillance of antenatal care users detects reduced transmission following elimination interventions in Mozambique

Brokhattingen,

Matambisso,

da Silva

et al. 2024

Nat Commun

Self Cite

View full text Add to dashboard Cite

show abstract

“…For continuous genomic surveillance of malaria, samples must be collected regularly, and, especially critical for lowresource settings, cost-e ciently. 2,13,14 Pregnant women attending their rst antenatal care (ANC) consultation are an easy-access subpopulation that could potentially serve as a sentinel group for malaria surveillance. 13,15,16 Besides low cost and easy accessibility, advantages of ANC-based surveillance include temporal continuity, known denominator populations, and the possibility of capturing asymptomatic infections.…”

Section: Introductionmentioning

confidence: 99%

Genomic malaria surveillance of antenatal care users detects reduced transmission following elimination interventions in Mozambique

Mayor,

Brokhattingen,

Matambisso

et al. 2023

Preprint

Self Cite

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Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compared the genetic structure of parasite populations sampled from 289 first ANC attendees and 93 children from the community in Mozambique between 2015 and 2019. Samples were amplicon sequenced targeting 165 microhaplotypes and 15 drug resistance genes. Metrics of genetic diversity and relatedness, as well as the prevalence of drug resistance markers, were consistent between the two populations. In an area targeted for elimination, intra-host genetic diversity declined in both populations (p=0.002-0.007), while for the ANC population, population genetic diversity was also lower (p=0.0004), and genetic relatedness between infections were higher (p=0.002) than control areas, indicating a recent reduction in the parasite population size. These results highlight the added value of genomic surveillance at ANC clinics to inform about changes in transmission beyond epidemiological data.

show abstract

Nanopore sequencing for malaria molecular surveillance: opportunities and challenges

Hamilton,

Ishengoma,

Parr

et al. 2023

Trends in Parasitology

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Sampling for malaria molecular surveillance

Cited by 7 publications

References 77 publications

Genomic malaria surveillance of antenatal care users detects reduced transmission following elimination interventions in Mozambique

Genomic malaria surveillance of antenatal care users detects reduced transmission following elimination interventions in Mozambique

Genomic malaria surveillance of antenatal care users detects reduced transmission following elimination interventions in Mozambique

Nanopore sequencing for malaria molecular surveillance: opportunities and challenges

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