The factors responsible for the maintenance of genetic variation among natural populations remain a mystery. Recent models of host±parasite co-evolution assume that parasites exert frequency-dependent selection on their hosts by favouring rare alleles that may confer resistance against infection. We tested this prediction in a comparative analysis that sought relationships between levels of genetic variation and the number of metazoan parasite species exploiting each host species. We used data on 40 species of North American freshwater ®shes. After controlling for sampling e ort and phylogenetic in¯uences, we found no relationship between genetic polymorphism and parasite species richness among ®sh species. However, we found a marginal negative correlation between parasite species richness and heterozygosity. This result goes against the prediction that increased selective pressure by parasites should be associated with higher levels of genetic variation. Instead, it suggests that parasites may be colonising host species showing low levels of genetic variation with greater success than genetically more variable host species. 7