1977
DOI: 10.1093/aesa/70.6.933
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Sampling and Distribution of Potato Leafhopper1 Eggs in Alfalfa Stems2

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Cited by 26 publications
(16 citation statements)
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“…Leaf disks were chemically cleared and stained following established procedures prior to visually counting all F. fusca eggs with the aid of a backlit stereomicroscope.…”
Section: Methodsmentioning
confidence: 99%
“…Leaf disks were chemically cleared and stained following established procedures prior to visually counting all F. fusca eggs with the aid of a backlit stereomicroscope.…”
Section: Methodsmentioning
confidence: 99%
“…After 3 days of confinement in cages within an indoor insectary (25 + 1 o C, 16 h photophase), adults were removed and counted by sex. Eggs inserted by ovipositing females in the plant tissues were counted after clearing the plants and staining the eggs with a lactophenol solution (Simonet & Pienkowski, 1977). This procedure was replicated five times for each plant species.…”
Section: Methodsmentioning
confidence: 99%
“…Eggs in the onion tissue were stained using a lacto phenol-acid fuchsin staining technique similar to one elaborated by Nuessly et al (1995) and Þrst described by Simonet and Pienkowski (1977). Onion leaßets were separated from the bulb, and all onion tissue was boiled for 3Ð 4 min in a 1:2:1:1:1 solution of 10% lactic acid, 50% glycerin solution, distilled water, saturated phenol buffered at pH 4.3, and 1 g/liter of acid fuchsin high purity biological stain.…”
Section: Micro-cagesmentioning
confidence: 99%