Sample preservation for determination of organic compounds: microwave versus freeze-drying

Popp, Marianne; Lied, Wolfgang; Meyer, Andreas; Richter, Andreas; Schiller, Petra; Schwitte, Hildegard

doi:10.1093/jxb/47.10.1469

Cited by 130 publications

(97 citation statements)

References 12 publications

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“…2). One possible mechanism that could account for the positive effect of N on photosynthesis in A. germinans is that N is invested in the osmotically compatible solute, glycine betaine (Popp et al 1996), resulting in improved water status of tissues. In either species, N could be invested in a greater number of growing apices (roots, shoots or reproductive structures), which in turn may enhance photosynthesis through increased sink strength (Cheeseman 1988;Farrar 1996).…”

Section: Photosynthesis-nitrogen Relationshipsmentioning

confidence: 99%

Photosynthetic performance and resource utilization of two mangrove species coexisting in a hypersaline scrub forest

2003

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In a hypersaline mangrove scrub forest in northern Florida, coexisting trees of Laguncularia racemosa and Avicennia germinans were either fertilized with nitrogen or phosphorus, or not fertilized (controls). We aimed to test whether nutrient additions differentially altered photosynthetic performance and resource utilization in these two species. In control trees, photosynthetic rates were higher in L. racemosa than A. germinans. However, leaf nitrogen concentrations were higher in A. germinans than L. racemosa. Avicennia germinans responded to fertilization with nitrogen by increasing leaf nitrogen concentrations and rates of photosynthesis such that they were equivalent to photosynthesis in L. racemosa. Laguncularia racemosa did not show a response to nitrogen additions. Neither species showed strong responses to phosphorus fertilization. Avicennia germinans had high photosynthetic water-use efficiency (photosynthesis/transpiration), but low photosynthetic nitrogen-use efficiency (photosynthesis/leaf nitrogen). In contrast, L. racemosa had comparatively low photosynthetic water use efficiency and high photosynthetic nitrogen use efficiency. Leaf level characteristics lead us to hypothesize that coexistence of A. germinans and L. racemosa should occur where nitrogen levels are low and salinity is moderate, or at least moderate for some period of the year.

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Section: Photosynthesis-nitrogen Relationshipsmentioning

confidence: 99%

Photosynthetic performance and resource utilization of two mangrove species coexisting in a hypersaline scrub forest

2003

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“…Leaf material was rapidly dried in a microwave (Popp et al, 1996) and subsequently dried in an oven for 24 h at 70°C. Dried material was ground in a ball mill and 50 to 100 mg were suspended in 1.5 mL of extraction buffer {100 mm HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid], 20 mm MgCl 2 , and 1 mm EDTA, pH 7.4}.…”

Section: Extractionmentioning

confidence: 99%

An Off-Line Implementation of the Stable Isotope Technique for Measurements of Alternative Respiratory Pathway Activities

2001

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In situ measurements of alternative respiratory pathway activity are needed to provide insight into the energy efficiency of plant metabolism under various conditions in the field. The only reliable method at present to measure alternative oxidase (AOX) activity is through measurement of changes in ␦ 18 O(O 2 ), which to date has only been used in laboratory environments. We have developed a cuvette system to measure partitioning of electrons to AOX that is suitable for off-line use and for field experiments. Plant samples are enclosed in airtight cuvettes and O 2 consumption is monitored. Gas samples from the cuvette are stored in evacuated gas containers until measurement of ␦ 18 O(O 2 ). We have validated this method using differing plant material to assess AOX activity. Fractionation factors were calculated from ␦ 18 O(O 2 ) measurements, which could be measured with an accuracy and precision to 0.1‰ and 0.3‰, respectively. Potential sources of error are discussed and quantified. Our method provides results similar to those obtained with laboratory incubations on-line to a mass spectrometer but greatly increases the potential for adoption of the stable isotope method.Measurements of partitioning of respiratory electron flow between the energy-conserving cytochrome oxidase (COX) pathway and the energy-inefficient alternative oxidase (AOX) pathway were initially quantified by assessing the effect on O 2 consumption of titrations with specific inhibitors of both respiratory pathways (see Møller et al., 1988). However, titration with inhibitors is associated with a number of problems. The most important is the assumption that inhibition of the alternative pathway would not affect the cytochrome pathway; this is not necessarily true (Wilson, 1988;Millar et al., 1995;. Therefore, AOX activity may be underestimated by titration. This problem was overcome by the noninvasive stable isotope method, which was initially developed by Guy et al. (1989) and has been perfected during the last decade (Robinson et al., 1992;Ribas-Carbo et al., 1997;Gonzàlez-Meler et al., 1999;Henry et al., 1999). This method relies on the different discrimination against the 18 O isotope by COX and AOX. Although inhibitors are used to measure the discrimination factor (D) of either oxidase, the actual measurement does not depend on inhibitors.The present on-line method (Robinson et al., 1992;Gonzàlez-Meler et al., 1999) uses a cuvette for incubation of plant material that is directly connected to a gas chromatograph and an isotope ratio mass spectrometer (IRMS), operating in continuous flow mode. This approach allows rapid and accurate measurements but has a few limitations. In particular, it is restricted to the very few laboratories worldwide that can acquire and maintain a dedicated on-line system. There is a great need to extend the use of the stable isotope approach to assess the role of AOX in the dissipation of excess reducing power under unfavorable growth conditions, such as low temperatures (Stewart et al., 1990;Vanlerberghe and McI...

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“…Este pre-tratamiento desnaturaliza las enzimas presentes en las muestras, evitando la degradación enzimática de carbohidratos que pudiera ocurrir entre el momento de recolección de las muestras y su secado en el laboratorio (Popp et al 1996). Este secado se efectuó en una estufa a 75 ºC hasta peso constante.…”

Section: Ii32 Contenido Relativo En Aguaunclassified

“…Se tomaron muestras en los tramos de ramas correspondientes a edades de 3 y 4 años, y en un plazo máximo de seis horas tras su recolección, se procedió a desnaturalizar las enzimas presentes en las muestras siguiendo las recomendaciones de Popp et al (1996), mediante choque térmico en un horno microondas (600 W de potencia, durante 90 s). Posteriormente, las muestras se secaron en una estufa a 75º C hasta peso constante.…”

Section: Vi27 Contenido Xilemático En Carbohidratos No Estructuralesunclassified

Análisis comparativo de la ecofisiología de dos especies emparentadas de abetos circunmediterráneos: rasgos adaptativos y compensación de factores en Abies pinsapo y Abies alba en el contexto de eventos de calentamiento climático

Serichol-Escobar

2014

ECOS

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Sample preservation for determination of organic compounds: microwave versus freeze-drying

Cited by 130 publications

References 12 publications

Photosynthetic performance and resource utilization of two mangrove species coexisting in a hypersaline scrub forest

Photosynthetic performance and resource utilization of two mangrove species coexisting in a hypersaline scrub forest

An Off-Line Implementation of the Stable Isotope Technique for Measurements of Alternative Respiratory Pathway Activities

Análisis comparativo de la ecofisiología de dos especies emparentadas de abetos circunmediterráneos: rasgos adaptativos y compensación de factores en Abies pinsapo y Abies alba en el contexto de eventos de calentamiento climático

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