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2021
DOI: 10.3791/63015-v
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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

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“…Additionally, advances in HPF technology allow for the light and electrical stimulation of samples moments before freezing 7 While the improvements in imaging capabilities and sample preparation instruments have been a major driving force behind the advancements in the field of cryoEM, a major hurdle in this research continues to be the hands-on preparation of samples. Truong et al 9 present a method that takes advantage of lipid monolayer formation at the air/ water interface. A lipid layer forms on the TEM grid and acts as a scaffold for the membrane proteins of interest.…”
mentioning
confidence: 99%
“…Additionally, advances in HPF technology allow for the light and electrical stimulation of samples moments before freezing 7 While the improvements in imaging capabilities and sample preparation instruments have been a major driving force behind the advancements in the field of cryoEM, a major hurdle in this research continues to be the hands-on preparation of samples. Truong et al 9 present a method that takes advantage of lipid monolayer formation at the air/ water interface. A lipid layer forms on the TEM grid and acts as a scaffold for the membrane proteins of interest.…”
mentioning
confidence: 99%
“…Additionally, advances in HPF technology allow for the light and electrical stimulation of samples moments before freezing 7 While the improvements in imaging capabilities and sample preparation instruments have been a major driving force behind the advancements in the field of cryoEM, a major hurdle in this research continues to be the hands-on preparation of samples. Truong et al 9 present a method that takes advantage of lipid monolayer formation at the air/ water interface. A lipid layer forms on the TEM grid and acts as a scaffold for the membrane proteins of interest.…”
mentioning
confidence: 99%