Sample preparation in glass capillaries for high-throughput biochemical analyses

Meldram, D.R.; Holl, Mark R.; Fisher, C. H.; Saini, Mohan S.; McGuire, Shawn K.; Ren, Tao; Pence, William H.; Moody, Stephen E.; Cunningham, D.L.; Donaldson, D.A.; Wiktor, Peter

doi:10.1109/coase.2005.1506737

Cited by 7 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meldrum et al demonstrated a capillary-based fluid handling system, ACAPELLA-5K (A5K), capable of processing genomic and chemical samples at a throughput of 5,000 preparations in 8 hours [2]. Kachel et al designed a custom-made automated system capable of isolating DNA plasmids at a rate of 1,600 plasmids in 12 hours [3].…”

Section: Introductionmentioning

confidence: 99%

Design and Preliminary Validation of a Rapid Automated Biodosimetry Tool for High Throughput Radiological Triage

Chen

Zhang

Wang

et al. 2009

Volume 3: ASME/IEEE 2009 International Conference on Mechatronic and Embedded Systems and Applications; 20th Reliability, Stres

View full text Add to dashboard Cite

This paper presents design, hardware, software, and parameter optimization for a novel robotic automation system. RABiT is a Rapid Automated Biodosimetry Tool for high throughput radiological triage. The design considerations guiding the hardware and software architecture are presented with focus on methods of communication, ease of implementation, and need for real-time control versus soft time control cycles. The design and parameter determination for a non-contact PVC capillary laser cutting system is presented. A novel approach for lymphocyte concentration estimation based on computer vision is reported. Experimental evaluations of the system components validate the success of our prototype system in achieving a throughput of 6,000 samples in a period of 18 hours.

show abstract

Section: Introductionmentioning

confidence: 99%

Design and Preliminary Validation of a Rapid Automated Biodosimetry Tool for High Throughput Radiological Triage

Chen

Zhang

Wang

et al. 2009

Volume 3: ASME/IEEE 2009 International Conference on Mechatronic and Embedded Systems and Applications; 20th Reliability, Stres

View full text Add to dashboard Cite

show abstract

“…Automation of the related steps in the X-ray crystallography pipeline has been pursued, though the most popular targets seem to have been sample preparation [10] and beamline mounting and alignment [11], [12]. Telerobotic techniques have also been investigated [13].…”

Section: Related Workmentioning

confidence: 99%

Two-stage robotic crystal mounting of protein crystals for X-ray data collection

Georgiev

Allen

2008

2008 IEEE International Conference on Automation Science and Engineering

View full text Add to dashboard Cite

Abstract-We present a new microrobotic system for a crystallographic task called protein crystal mounting, which is the transfer of a crystal from its growth solution onto a tool designed to hold it for X-ray data collection. The system operates autonomously using feedback from a camera looking at the workspace through a microscope. Visual tracking is employed to monitor the location of the selected crystal and control a range of actuators for successful extraction. The task is performed in two stages: First, the crystal is taken from its drop into a pipette and is transported into a cryoprotecting liquid. Second, while in the cryoprotector, it is transferred from the pipette onto a tool to be used for data collection. Key features of this approach are the increased robustness of the system and the avoidance of the damaging effect of direct exposure of the crystal to room conditions. This work is part of a larger effort we have directed at the automation of the high-throughput crystallographic pipeline.

show abstract

“…From Kachel et al's research, a custom-made automated system was designed to isolate deoxyribonucleic acid (DNA) plasmids [1]. Meldrum et al designed a system to prepare glass capillary sample [2]. The proposed solutions, however, only focused on sample handling and imaging system, but not automation of the entire protocol.…”

mentioning

confidence: 99%

Liquid Handling Optimization in High-Throughput Biodosimetry Tool

Bian

Tsui

Repin

et al. 2016

Journal of Medical Devices

View full text Add to dashboard Cite

Due to the need of high-speed and efficient biodosimetric assays for triage and therapy in the event of radiological or nuclear attack, a robotically based automated biodosimetry tool (RABiT) has been developed over the past few years. Adapting the micronucleus assay from filter plates to V-shaped plates presented challenges in the liquid handling, namely, cell splashing out of the V-shaped well plate during the cell harvesting, poor cell distribution on the bottom of the image plate during the dispensing, and cell loss from the image plate during the aspiration in the liquid handling process. Experimental and numerical investigations were carried out to better understand the phenomena and mitigate the problems. Surface tension and contact angle among the fluids and the plate wall were accounted for in the discrete and multiphase numerical models. Experimental conditions were optimized based on the numerical results showing the relationship between nozzle speed and amount of splashed liquid, and the relationship between aspiration speed and number of escaped cells. Using these optimized parameters, numbers of micronuclei in binucleated cells showed the same dose dependence in the RABiT-prepared samples as those in the manually prepared ones. Micronucleus assay protocol was fully realized on RABiT.

show abstract

Sample preparation in glass capillaries for high-throughput biochemical analyses

Cited by 7 publications

References 12 publications

Design and Preliminary Validation of a Rapid Automated Biodosimetry Tool for High Throughput Radiological Triage

Design and Preliminary Validation of a Rapid Automated Biodosimetry Tool for High Throughput Radiological Triage

Two-stage robotic crystal mounting of protein crystals for X-ray data collection

Liquid Handling Optimization in High-Throughput Biodosimetry Tool

Contact Info

Product

Resources

About