Sample preparation for the analysis of isoflavones from soybeans and soy foods

Rostagno, Maurı́cio A.; Villares, Ana; Guillamón, Eva; García–Lafuente, Ana; Martínez, Julian

doi:10.1016/j.chroma.2008.11.035

Cited by 168 publications

(137 citation statements)

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“…Sample preparation. The protocol used for the extraction of target analytes from the solid samples was based on Rostagno et al (2003Rostagno et al ( , 2009. Briefly, the ultrasound-assisted extraction method used involved the three sequential extraction of 0.5 g of sample, with 15 ml of 50, 75, and 100% methanol, each of them at 60°C for 20 minutes.…”

Section: Methodsmentioning

confidence: 99%

Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea

Manchón¹,

Mateo-Vivaracho²,

D’Arrigo³

et al. 2013

Czech J. Food Sci.

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Manchón N., Mateo-Vivaracho L., D'Arrigo M., García-Lafuente A., Guillamón E., Villares A., Rostagno M.A. (2013): Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea. Czech J. Food Sci., 31: 483-500.A previously developed method of HPLC-DAD-Fl has been used for the determination of phytochemical profiles in different types of drinks: instant coffee, soft drinks, energy drinks, and different types of tea (green, white, black, and red tea). Using data on the concentrations of 20 main phytochemicals (phenolic acids, flavan-3-ols, flavonols, flavones, and alkaloids) it was possible to identify most of the sample types. Chlorogenic and caffeic acids and caffeine are the main target compounds in instant coffee; in soft and energy drinks, only caffeine was found. Tea has a more complex phytochemical composition. Unfermented tea is mainly composed of flavan-3-ols and alkaloids, with a high caffeine concentration. Black tea is composed of alkaloids and low levels of flavan-3-ols, which are affected by oxidative reactions during the fermentation. Flavonols are present in lower concentrations in all kinds of teas. The identified phytochemical distribution patterns were used to correctly differentiate instant coffee, soft drinks, energy drinks, unfermented tea and fermented tea (within fermented tea, black tea from red tea can also be differentiated).

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Section: Methodsmentioning

confidence: 99%

Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea

Manchón¹,

Mateo-Vivaracho²,

D’Arrigo³

et al. 2013

Czech J. Food Sci.

Self Cite

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“…Rostagno et al 43 reported that the stability of isoflavones is influenced by several factors, including processing temperature, duration of heating, photosensitization under light exposure, pH, and the presence of microorganisms. Our study used a nanoprecipitation method to prepare 734N formulation using high-speed homogenization, and these processes easily generated higher processing temperatures and exposure to light.…”

Section: Notesmentioning

confidence: 99%

Design of acid-responsive polymeric nanoparticles for 7,3',4'-trihydroxyisoflavone topical administration

Huang¹,

Hu²,

Lee³

et al. 2016

IJN

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7,3′,4′-Trihydroxyisoflavone (734THIF) is a secondary metabolite of daidzein and has been recently found to possess antioxidant, melanin inhibition, and skin cancer chemopreventive activities. However, the poor water solubility of 734THIF impedes its absorption and skin penetration and, therefore, limits its pharmacological effects when applied topically to the skin. We seek to use the nanoprecipitation method to prepare optimal eudragit E100 (EE)–polyvinyl alcohol (PVA)-loaded 734THIF nanoparticles (734N) to improve its physicochemical properties and thereby increase its water solubility, skin penetration, and biological activities. EE–PVA-loaded 734THIF nanoparticles (734N) were prepared, and their morphology and particle size were evaluated using a particle size analyzer and by electron microscopy. The drug loading and encapsulation efficiencies and in vitro solubility were determined using high-performance liquid chromatography. Hydrogen-bond formation was evaluated by 1 H-nuclear magnetic resonance and Fourier transform infrared spectroscopy, and crystalline-to-amorphous transformation was determined by differential scanning calorimetry and X-ray diffractometry. In vitro skin penetration was analyzed using fresh pig skin mounted on Franz diffusion cells, and cytotoxicity against human keratinocyte HaCaT cells was evaluated using the MTT assay. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl-free radical scavenging ability. EE–PVA-loaded 734THIF nanoparticles showed good drug loading and encapsulation efficiencies and were characterized by improved physicochemical properties, including reduction in particle size, amorphous transformation, and intermolecular hydrogen-bond formation. This is associated with increased water solubility and enhanced in vitro skin penetration, with no cytotoxicity toward HaCaT cells. In addition, 734THIF nanoparticles retained their antioxidant activity. In conclusion, 734THIF nanoparticles are characterized by improved physicochemical properties, increased water solubility, and enhanced skin penetration, and these may have potential use in the future as a topical delivery formulation for the treatment of skin diseases.

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“…Generally, the analytical determinations of phytoestrogens in food have been carried out by LC-UV, LC-MS, or GC-MS [13][14][15][16][17][18][19][20]. Their analysis in complex matrices obviously requires sample pretreatment procedures to simplify the matrix.…”

Section: Introductionmentioning

confidence: 99%

Determination of Isoflavones in Soybean Flour by Matrix Solid-Phase Dispersion Extraction and Liquid Chromatography with UV-Diode Array Detection

Aresta

Cotugno

Massari

et al. 2017

Journal of Food Quality

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A new analytical method, based on liquid chromatography (LC) with UV-diode array detection, for the simultaneous determination of daidzein, genistein, and glycitein and their 7-O--D-glucopyranoside (daidzin, genistin, and glycitin, resp.) has been successfully developed. All the calibration curves showed good linearity within the concentration range 0.02-2 g/ml. The limits of detection and quantitation were 0.057 (genistin and glycitein), 0.124 g/ml (genistein), 0.190 g/ml (genistin and glycitein), and 0.410 g/ml (genistein), respectively. Within-day and between-days precision were found not to be significantly different according to an Ftest; values (% RSD) ranged from 2.0 to 2.9%. Extraction and clean-up of soybean flour samples were carried out using matrix solid-phase dispersion extraction (MSPD). The main parameters affecting extraction yield, such as dispersant, type and amount of additives, cosorbent, and extractive solvent, were evaluated and optimized. The average recovery values were between 85.7 and 102.6%. The target isoflavone concentration levels estimated in this work fit existing literature data and were comprised between 39.3 and 345.3 g/g. The whole procedure has proved to be simple, accurate, precise, and cheap.

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Sample preparation for the analysis of isoflavones from soybeans and soy foods

Cited by 168 publications

References 176 publications

Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea

Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea

Design of acid-responsive polymeric nanoparticles for 7,3',4'-trihydroxyisoflavone topical administration

Determination of Isoflavones in Soybean Flour by Matrix Solid-Phase Dispersion Extraction and Liquid Chromatography with UV-Diode Array Detection

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Sample preparation for the analysis of isoflavones from soybeans and soy foods

Cited by 168 publications

References 176 publications

Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea

Distribution patterns of polyphenols and alkaloids in instant coffee, soft and energy drinks, and tea

Design of acid-responsive polymeric nanoparticles for 7,3&#39;,4&#39;-trihydroxyisoflavone topical administration

Determination of Isoflavones in Soybean Flour by Matrix Solid-Phase Dispersion Extraction and Liquid Chromatography with UV-Diode Array Detection

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Design of acid-responsive polymeric nanoparticles for 7,3',4'-trihydroxyisoflavone topical administration