Sample preparation for scanning electron microscopy of plant surfaces—Horses for courses

Pathan, Amin K.; Bond, J.M.; Gaskin, R.E.

doi:10.1016/j.micron.2008.05.006

Cited by 146 publications

(103 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Samples were then coated with gold using a sputter coater (Jeol JFC-1100E, Japan). Finally, samples were scanned using a scanning electron microscope (Jeol model JSM 5410, Japan) [29].…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

View full text Add to dashboard Cite

In vitro cultures of Leucojum aestivum are considered as an alternative for the production of galanthamine, which is used for the symptomatic treatment of Alzheimer’s disease. We studied the effects of auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 4-amino-3,5,6-trichloropicolinic acid (picloram), 3,6-dichloro-o-anisic acid (dicamba) at concentrations of 25 and 50 µM on the induction of embryogenic callus and its capacity to induce somatic embryogenesis and alkaloid accumulation. The embryogenic response of the explants was from 30% for 25 µM of dicamba to 100% for picloram (for both 25 and 50 µM). 2,4-D (50 µM) stimulated greater callus proliferation and somatic embryo induction as compared to the other auxins. Polyethylene glycol (PEG) stimulated somatic embryo maturation. Callus grown on media containing 50 µM of auxins produced fewer phenolic compounds as compared with callus grown on media containing 25 µM of auxins. GC-MS analyses showed seven alkaloids in the in vivo bulbs and two to four in callus culture. Galanthamine was detected in callus cultivated with 2,4-D (25, 50 µM), picloram (25 µM), and dicamba (50 µM). Other alkaloids, trisphaeridine, tazettine, and 11-hydroxyvittatine were accumulated only in callus growing on medium with picloram (50 µM).

show abstract

“…Samples were then coated with gold using a sputter coater (Jeol JFC-1100E, Japan). Finally, samples were scanned using a scanning electron microscope (Jeol model JSM 5410, Japan) [29].…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

et al. 2013

View full text Add to dashboard Cite

show abstract

“…A fresh leaf of each faba bean cultivar was observed for SEM in their native-hydrated state with 1045× magnification at 7.36 kv and a pressure of 33 Pa (Pathan et al, 2008).…”

Section: Plant Materialsmentioning

confidence: 99%

Electrical Penetration Graph monitored feeding behavior of cowpea aphid, Aphis craccivora Koch. (Hemiptera:Aphididae), on faba bean, Vicia faba L. (Fabaceae), cultivars

Soffan

Aldawood

2016

Turkish Journal of Entomology

View full text Add to dashboard Cite

SummaryFeeding behavior of cowpea aphid, Aphis craccivora Koch. (Hemiptera: Aphididae), was evaluated on five faba bean, Vicia faba L. (Fabaceae), cultivars, including the resistant cultivar Gazira2, by using the Electrical Penetration Graph (EPG) technique. Experiments were conducted on whole plants (WP) and detached leaves (DL). Fifteen parameters of EPG recording were selected for statistical analysis. Two-way factorial ANOVA showed that the EPG parameter values in WP and DL were significantly different, while the cultivars difference occurred mostly in the WP tissue, as indicated through one-way ANOVA Kruskal-Wallis analysis. Cowpea aphid feeding behavior on WP Gazira2 resistant cultivar had the longest duration of stylet derailment (waveform F) and total probing, but lowest in total duration of pathway (waveform C), xylem ingestion (waveform G),and number of probes. Speculatively, longest duration of waveform F, which had a relation with the mechanical disturbance during feeding, probably is one of the possible resistant factors in Gazira2 cultivar, however this remains intriguing and needs further study.

show abstract

“…Nevertheless, since it is a simple method, it may be an alternative when the objective of documentation is only observation of the anatomy and distribution of the constituent elements of the stem. Critical point drying with CO 2 is the most common method of tissue dehydration for preparation of samples for microscopy (PATHAN et al, 2008). Critical point drying with CO 2 proved to be adequate in preservation of both the structures of the gerbera stems ( Figure 9) and the microorganisms and exopolysaccharides of bacteria present in the samples (Figure 10 and 11).…”

Section: Dryingmentioning

confidence: 99%

Methods of preparing flower stem samples for scanning electron microscopy

Spricigo¹,

Trento²,

Bresolin³

et al. 2015

View full text Add to dashboard Cite

Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector, helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool that allows viewing of flower structures and also microorganisms. The aim of this study was to evaluate methods of preparing flower stem samples for viewing in SEM as a support for studies on postharvest cut flowers. Ways of cutting, fixing, and drying samples were tested. Cutting with a stainless steel blade and through freeze-fracture were tested; fixation was carried out without the use of osmium tetroxide (OsO4); and drying of the samples was performed through freeze-drying and through critical point dryingwithCO2. Cutting with a stainless steel blade proved to be a satisfactory method for stem samples, with low cost and simple application compared to freeze-fracturing. Good fixation and high image contrast were obtained without the use of osmium tetroxide, thus avoiding the use of this toxic compound. Freeze-drying allowed the structure and morphological composition to be viewed, while critical point drying withCO2 preserved the microorganisms present in the samples.Keywords: gerbera,stainless steel blade, freeze-fracturing, freeze-drying, critical point with CO 2 . RESUMOMétodos de preparação de amostras de hastes florais para microscopia eletrônica de varredura O Brasil possui grande capacidade de expansão na área da floricultura. Estudos em pós-colheita de flores de corte contribuem para o desenvolvimento do setor, auxiliando na manutenção da qualidade da produção nacional. A microscopia eletrônica de varredura (MEV) é uma ferramenta potente que permite a visualização das estruturas florais e também de microrganismos. Este trabalho objetivou avaliar métodos de preparação de amostras de hastes florais para visualização de imagens em MEV como suporte em estudos de pós-colheita de flores cortadas. Foram testadas formas de obtenção de corte, fixação e secagem das amostras. Os tipos de cortes testados foram por meio de lâmina de aço inoxidável e criofratura, a fixação foi realizada sem a utilização do tetróxido de ósmio (OsO 4 ) e a secagem das amostras foi realizada por liofilização e por ponto crítico de CO 2 . O corte com a lâmina de aço inoxidável, demonstrou ser um método satisfatório para amostragem das hastes, de baixo custo e simples aplicação com relação a criofratura. Obteve-se uma boa fixação e alto contraste das imagens sem o uso do tetróxido de ósmio, desta forma, evitou-se a utilização deste composto tóxico. A secagem por liofilização permitiu a visualização da estrutura e composição morfológica, enquanto a secagem por ponto crítico de CO2 conservou os microorganismos presentes nas amostras. Palavras-chave: gérbera, lâmina de aço inoxidável, criofratura, liofilização, ponto crítico de CO 2 .(1) Trabalho recebido para publicação em 26

show abstract

Sample preparation for scanning electron microscopy of plant surfaces—Horses for courses

Cited by 146 publications

References 32 publications

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus

Electrical Penetration Graph monitored feeding behavior of cowpea aphid, Aphis craccivora Koch. (Hemiptera:Aphididae), on faba bean, Vicia faba L. (Fabaceae), cultivars

Methods of preparing flower stem samples for scanning electron microscopy

Contact Info

Product

Resources

About