Sample preparation for mass spectrometry imaging: Small mistakes can lead to big consequences

Goodwin, Richard J. A.

doi:10.1016/j.jprot.2012.04.012

Cited by 264 publications

(257 citation statements)

References 142 publications

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“…In are the object of an imaging study. In IMS rigorous sample preparation is vital to achieve the most accurate, reproducible and validated data as possible [65]. In most cases dissection of the sample is generally followed by freezing the tissue (in liquid nitrogen or isopentane) and storing at -80 °C until use.…”

Section: Imaging Msmentioning

confidence: 99%

Direct Mass Spectrometry-Based Approaches in Metabolomics

Ibáñez

García‐Cañas

Valdés

et al. 2014

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites

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Metabolomics is one of the newest omics technologies concerned with the identification and quantification of small molecules in a high-throughput manner. Considering the number of different types of metabolites present in a wide dynamic range of concentrations in any single living system, still actual analytical technologies can only capture a part of the metabolome.Currently MS-based approaches yield a higher sensitivity than NMR when analyzing minimal amounts of complex mixtures. Most MS-based approaches in metabolomics involve a physical/chemical purification/fractionation prior to MS analysis, to avoid sample matrix effects, at the expenses of low high-throughput performance. In the quest to achieve the maximum highthroughput production of metabolite information in the largest possible number of samples an extensive array of direct ionization or desorption/ionization techniques have been developed and combined. In the present Chapter an overview of the main desorption/ionization techniques coupled to MS applied to direct metabolite profiling or fingerprinting is presented.

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Section: Imaging Msmentioning

confidence: 99%

Direct Mass Spectrometry-Based Approaches in Metabolomics

Ibáñez

García‐Cañas

Valdés

et al. 2014

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites

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“…Applying a suitable matrix layer on such samples can be a difficult task, which is further complicated by the components of the microbial cells and their extracellular matter. Consequently, several sample preparation techniques may be used, mainly based on methods established for tissue imaging [9]. Following the approach of applying dry matrix on a tissue sample [10,11], sieving matrix powder onto wet agar samples has become a common sample preparation technique for microbial IMS in our lab [12].…”

Section: Introductionmentioning

confidence: 99%

Homogeneous Matrix Deposition on Dried Agar for MALDI Imaging Mass Spectrometry of Microbial Cultures

Hoffmann

Dorrestein

2015

J. Am. Soc. Mass Spectrom.

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Abstract. Matrix deposition on agar-based microbial colonies for MALDI imaging mass spectrometry is often complicated by the complex media on which microbes are grown. This Application Note demonstrates how consecutive short spray pulses of a matrix solution can form an evenly closed matrix layer on dried agar. Compared with sieving dry matrix onto wet agar, this method supports analyte cocrystallization, which results in significantly more signals, higher signal-to-noise ratios, and improved ionization efficiency. The even matrix layer improves spot-to-spot precision of measured m/z values when using TOF mass spectrometers. With this technique, we established reproducible imaging mass spectrometry of myxobacterial cultures on nutrient-rich cultivation media, which was not possible with the sieving technique.

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“…For example, sample preparation for matrix assisted laser desorption ionization-mass spectrometry analysis of soft tissue has been discussed in literature. 16 For ToF-SIMS analysis, only a few studies have been published so far on the effect of sample preparation, typically dealing with the preparation of cells or soft tissue. [17][18][19][20][21] To the best of our knowledge, no study on the sample preparation of bone tissue for ToF-SIMS analysis has been published so far.…”

Section: Introductionmentioning

confidence: 99%

Time of flight secondary ion mass spectrometry of bone—Impact of sample preparation and measurement conditions

Henß

Hild²,

Rohnke

et al. 2015

Biointerphases

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Time of flight secondary ion mass spectrometry (ToF-SIMS) enables the simultaneous detection of organic and inorganic ions and fragments with high mass and spatial resolution. Due to recent technical developments, ToF-SIMS has been increasingly applied in the life sciences where sample preparation plays an eminent role for the quality of the analytical results. This paper focusses on sample preparation of bone tissue and its impact on ToF-SIMS analysis. The analysis of bone is important for the understanding of bone diseases and the development of replacement materials and new drugs for the cure of diseased bone. The main purpose of this paper is to find out which preparation process is best suited for ToF-SIMS analysis of bone tissue in order to obtain reliable and reproducible analytical results. The influence of the embedding process on the different components of bone is evaluated using principal component analysis. It is shown that epoxy resin as well as methacrylate based plastics (Epon and Technovit) as embedding materials do not infiltrate the mineralized tissue and that cut sections are better suited for the ToF-SIMS analysis than ground sections. In case of ground samples, a resin layer is smeared over the sample surface due to the polishing step and overlap of peaks is found. Beside some signals of fatty acids in the negative ion mode, the analysis of native, not embedded samples does not provide any advantage. The influence of bismuth bombardment and O 2 flooding on the signal intensity of organic and inorganic fragments due to the variation of the ionization probability is additionally discussed. As C 60 sputtering has to be applied to remove the smeared resin layer, its effect especially on the organic fragments of the bone is analyzed and described herein.

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Sample preparation for mass spectrometry imaging: Small mistakes can lead to big consequences

Cited by 264 publications

References 142 publications

Direct Mass Spectrometry-Based Approaches in Metabolomics

Direct Mass Spectrometry-Based Approaches in Metabolomics

Homogeneous Matrix Deposition on Dried Agar for MALDI Imaging Mass Spectrometry of Microbial Cultures

Time of flight secondary ion mass spectrometry of bone—Impact of sample preparation and measurement conditions

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