Sample Preparation for LC‐MS Bioanalysis of Proteins

Merbel, Nico van de

doi:10.1002/9781119274315.ch24

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2023

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Cited by 2 publications

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“…Currently, one of the main methods for assessing the composition of crude venom (“direct” approach) is liquid chromatography with tandem mass spectrometry (LC/MS/MS), which makes it possible to determine what protein families are present in the venom. 34,35 This method is not without drawbacks: many proteins required de novo MS sequence analysis with homology searches to place protein in a particular venom toxin family; venom gland transcriptome data, although of great utility in MS identification of venom proteins do not necessarily reflect the proteome of the venom. 34 Techniques such as chromatography and gel electrophoresis are used to separate venom proteins and typically require following mass spectrometric analysis for protein identification.…”

Section: Discussionmentioning

confidence: 99%

Differentiation of snake venom using Raman spectroscopic analysis

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Differentiation of snake venom using Raman spectroscopic analysis

et al. 2023

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Quantitative Bioanalysis of Proteins by Digestion and LC–MS/MS: The Use of Multiple Signature Peptides

Sleumer,

Kema,

van de Merbel

2023

Bioanalysis

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The use of multiple signature peptides for the quantification of proteins by digestion and LC–MS/MS is reviewed and evaluated here. A distinction is made based on the purpose of the use of multiple peptides: confirmation of the protein concentration, discrimination between different protein forms or species and in vivo biotransformation. Most reports that describe methods with at least two peptides use these for confirmation, but it is not always mentioned how the peptides are used and how possible differences in concentration between the peptides are handled. Differences in concentration are often reported in the case of monitoring different protein forms or in vivo biotransformation, and this offers insight into the biological fate of the protein.

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Sample Preparation for LC‐MS Bioanalysis of Proteins

Abstract: Merbel, van de, N. (2019). Sample preparation for LC-MS bioanalysis of proteins. In W. Li, W. Jian, & Y. Fu (Eds.), Sample preparation in First Edition (1st ed.,. Wiley.

Cited by 2 publications

References 58 publications

Differentiation of snake venom using Raman spectroscopic analysis

Differentiation of snake venom using Raman spectroscopic analysis

Quantitative Bioanalysis of Proteins by Digestion and LC–MS/MS: The Use of Multiple Signature Peptides

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