Sample preparation followed by HPLC under harmless 100% aqueous conditions for determination of oxytetracycline in milk and eggs

Furusawa, Naoto

doi:10.1002/jssc.200301688

Cited by 12 publications

(7 citation statements)

References 21 publications

(4 reference statements)

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“…The centrifugal ultrafiltration procedure is easy to use and requires little time and solvent, but usually results in high recovery and reproducibility [12,13]. Samples containing NOV were prepared by methanol extraction of chicken muscle followed by a standard centrifugal ultrafiltration procedure.…”

Section: Centrifugal Ultrafiltration Of Novobiocin In Biologic Samplesmentioning

confidence: 99%

LC-MS/MS and centrifugal ultrafiltration method for the determination of novobiocin in chicken, fish tissues, milk and human serum

Inoue

Nitta

Hino

et al. 2009

Journal of Chromatography B

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Section: Centrifugal Ultrafiltration Of Novobiocin In Biologic Samplesmentioning

confidence: 99%

LC-MS/MS and centrifugal ultrafiltration method for the determination of novobiocin in chicken, fish tissues, milk and human serum

Inoue

Nitta

Hino

et al. 2009

Journal of Chromatography B

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“…This procedure allows a minimal consumption of serum sample (about 100 lL), is simple, rapid and easily applicable in a routine analysis laboratory providing a satisfactory clean up. Ultrafiltration has been extensively used for the isolation and enrichment of proteins [37,38] and for clinical diagnosis of several diseases [36]; very few applications as clean-up tool have been reported [39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Bile Acids Profile in Human Serum by Ultrafiltration Clean-up and LC-MS/MS

et al. 2012

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Bile acids (BAs) are useful biomarkers for the diagnosis of many diseases. The pathologies related to bile acid synthesis are often expressed in the first years of life and may lead to serious liver injury. Here we present a sensitive and rapid method for the analysis of the main 14 bile acids in human serum by liquid chromatography-tandem mass spectrometry. The chromatographic separation is performed using a core-shell column which provides improved separation, highly desirable considering the small structural differences among the analytes. All isomeric BAs of interest were resolved in less than 9 min. Sample pretreatment consisted in ultrafiltration of serum after addition of methanol by means of centrifugal filter devices. The calculated LOQs ranged between 2 and 5 ng mL(-1) with linearity of the calibration curves in the 5-5,000 ng mL(-1) range for all the BAs. The extraction recoveries for all the analytes were higher than 80 %. Intra-day and inter-day coefficients of variation were all below 15 %. The method proposed has been validated and has been applied for the analysis of serum of pediatric patients. This simple procedure allowed minimal consumption of serum sample (about 100 mu L) and a rapid assay, easily implementable in routine analysis.[...

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“…Antibiotics of the TC series are usually determined by microbiological approaches [5], immunoassays [6], HPLC [7][8][9][10], and CE [11][12][13][14]. Microbiological approaches and immunoassays are expensive and time-consuming with low sensitivity and poor specificity.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic total analysis of trace tetracycline antibiotics in a microchip with amperometry

et al. 2009

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The preconcentration, separation, and electrochemical detection of a series of tetracycline (TC) antibiotics in a microfluidic channel were preformed. The electrophoretic experimental parameters to analyze TC, oxytetracycline, chlortetracycline, and doxycycline antibiotics were investigated with a cellulose-dsDNA-modified carbon paste electrode. Modification of the electrode improved detection performance by enhancing the signal-to-noise characteristics without surface fouling of the electrode. Field-amplified sample stacking and field-amplified sample injection techniques were employed for on-chip preconcentration of the TC series. The sensitivity of the method was improved 10 900-fold when compared with conventional MEKC-electrochemical detection analysis. The overall recoveries for TC, oxytetracycline, chlortetracycline, and doxycycline were 87, 89, 87, and 81%, respectively, with 6.0% RSD. The limits of detection for the series were estimated between 1.5 and 4.3 nM. Applicability of the method to beef samples was successfully demonstrated.

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Sample preparation followed by HPLC under harmless 100% aqueous conditions for determination of oxytetracycline in milk and eggs

Cited by 12 publications

References 21 publications

LC-MS/MS and centrifugal ultrafiltration method for the determination of novobiocin in chicken, fish tissues, milk and human serum

LC-MS/MS and centrifugal ultrafiltration method for the determination of novobiocin in chicken, fish tissues, milk and human serum

Analysis of Bile Acids Profile in Human Serum by Ultrafiltration Clean-up and LC-MS/MS

Electrophoretic total analysis of trace tetracycline antibiotics in a microchip with amperometry

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