Sample preparation and imaging procedures for fast and multiplexed superresolution microscopy with DNA-PAINT-ERS

Abstract

DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.

Fast and multiplexed superresolution imaging with DNA-PAINT-ERS

“…Materials. A step-by-step guide for generating the labeling reagents, preparing immunostained samples, and performing image acquisition and initial analysis can be found at Protocol Exchange 21 . All DNA oligonucleotides were synthesized from Integrated DNA Technologies.…”