Sample handling in surface sensitive chemical and biological sensing: A practical review of basic fluidics and analyte transport

Orgován, Norbert; Patko, Daniel; Hős, Csaba; Kurunczi, Sándor; Szabó, Bálint; Ramsden, Jeremy J.; Horváth, Róbert

doi:10.1016/j.cis.2014.03.011

Cited by 32 publications

(36 citation statements)

References 102 publications

(142 reference statements)

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“…orders of magnitude greater than the size of the protein molecule and there is no hydromechanical force on the protein [59,60].…”

Section: Protein Adsorption Experiments On the Tnt-coated And Uncoatementioning

confidence: 99%

“…Measurements were made with HEK293 cells using the laboratory-built OWLS device dedicated to live cell studies, with the temperature at the OWLS chip being kept at 37 • C throughout the experiments and a closed cuvette without flow placed over the sensing area enabling the cells to be introduced using a pipette [60]. First, the position of the incoupled resonant peaks were recorded when the cuvette contained buffered cell culture medium without cells.…”

Section: Cell Spreading Kinetics and Adhesion Strength On The Tnt-coamentioning

confidence: 99%

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Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Nádor

Orgován

Fried

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Please cite this article in press as: J. Nador, et al., Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips, Colloids Surf. a b s t r a c tA new type of titanate nanotube (TNT) coating is investigated for exploitation in biosensor applications. The TNT layers were prepared from stable but additive-free sols without applying any binding compounds. The simple, fast spin-coating process was carried out at room temperature, and resulted in well-formed films around 10 nm thick. The films are highly transparent as expected from their nanostructure and may, therefore, be useful as coatings for surface-sensitive optical biosensors to enhance the specific surface area. In addition, these novel coatings could be applied to medical implant surfaces to control cellular adhesion. Their morphology and structure was characterized by spectroscopic ellipsometry (SE) and atomic force microscopy (AFM), and their chemical state by X-ray photoelectron spectroscopy (XPS). For quantitative surface adhesion studies, the films were prepared on optical waveguides. The coated waveguides were shown to still guide light; thus, their sensing capability remains. Protein adsorption and cell adhesion studies on the titanate nanotube films and on smooth control surfaces revealed that the nanostructured titanate enhanced the adsorption of albumin; furthermore, the coatings considerably enhanced the adhesion of living mammalian cells (human embryonic kidney and preosteoblast).

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“…orders of magnitude greater than the size of the protein molecule and there is no hydromechanical force on the protein [59,60].…”

Section: Protein Adsorption Experiments On the Tnt-coated And Uncoatementioning

confidence: 99%

Section: Cell Spreading Kinetics and Adhesion Strength On The Tnt-coamentioning

confidence: 99%

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Nádor

Orgován

Fried

et al. 2014

Colloids and Surfaces B: Biointerfaces

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“…Using the measured refractive indices and concentrations of the dilution series, we determined the refractive index increments of the solutions. 20,21 This information was necessary to estimate the mass of the adsorbed particles, which was determined according to de Feijter's formula 27 as M = d A (n A − n c )/(dn/dc), where d A , n A , and n c are thickness of adlayer, refractive index of adlayer, and refractive index of cover layer, respectively, and the averaged refractive index increment of AuMUA and AuTMA was dn/dc = 0.3845 cm 3 /g.…”

Section: Resultsmentioning

confidence: 99%

Label-Free in Situ Optical Monitoring of the Adsorption of Oppositely Charged Metal Nanoparticles

et al. 2014

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ABSTRACT:The mechanism of alternating deposition of oppositely charged gold nanoparticles (AuNPs) was investigated by optical waveguide lightmode spectroscopy (OWLS). OWLS allows monitoring of the kinetics of layer-by-layer (LbL) adsorption of positively and negatively charged nanoparticles in real time without using any labels so that the dynamics of layer formation can be revealed. Positively charged NPs that are already deposited on a negatively charged glass substrate strongly facilitate the adsorption of the negatively charged particles. The morphology of the adsorbed layer was also investigated with atomic force microscopy (AFM). AFM revealed that the interaction between oppositely charged particles results in the formation of NP clusters with sizes varying between 100 and 6000 NPs. The cluster size distribution is found to be an exponentially decaying function, and we propose a simple theory to explain this finding.

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“…The waveguides were assembled into a cylindrical flow-through cuvette of hemispherical crosssection so as to form the floor of the cuvette [35]. Throughout the procedures described in §2.3 the incoupling angles (from which the effective refractive indices N were immediately calculated [36,37]) were recorded at least every 30 s for the zeroth transverse magnetic (TM) and transverse electric (TE) modes using an OWLS 110 instrument (MicroVacuum).…”

Section: Waveguiding Substrata and Owlsmentioning

confidence: 99%

Surface rearrangement of adsorbed EGCG–mucin complexes on hydrophilic surfaces

Horváth

Yakubov

Ramsden

2017

International Journal of Biological Macromolecules

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The kinetic adsorption-desorption behaviour of porcine gastric mucin in the presence of physiologically relevant concentrations of the polyphenol epigallocatechin gallate (EGCG) was investigated using high-resolution kinetic optical waveguide lightmode spectroscopy (OWLS) and atomic force microscopy (AFM). Comparison with dynamic light scattering results from EGCGmucin mixtures indicates that discrete particles are formed whose size increases with increasing EGCG:mucin ratio. These particles are deduced to be the adsorbing entities, which fuse on the surface to form complex surface layers. At low molar EGCG:mucin ratios (< 1000), aggregates fuse on the surface to form a monolayer similar to one of pure mucin. With increasing EGCG concentration, the surface assembly of aggregates becomes consistent with their rearrangement and spreading in the shape of a spherical segment. At the highest molar ratios investigated (> 12 000) the particles begin to destabilize. The presence of EGCG leads to birefringence hysteresis during adsorption-desorption, indicating structural rearrangement, even at molar ratios 1000. The intensification of the phenomenon with increasing EGCG:mucin ratio mimics what was previously observed with the increase of mucin concentration in an EGCG-free system.

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Sample handling in surface sensitive chemical and biological sensing: A practical review of basic fluidics and analyte transport

Cited by 32 publications

References 102 publications

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Label-Free in Situ Optical Monitoring of the Adsorption of Oppositely Charged Metal Nanoparticles

Surface rearrangement of adsorbed EGCG–mucin complexes on hydrophilic surfaces

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