Sample gating in open tubular and packed capillaries for high-speed liquid chromatography

Monnig, Curtis A.; Dohmeier, Daniel M.; Jorgenson, James W.

doi:10.1021/ac00008a014

Cited by 45 publications

(12 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Separation of QDs‐BSA conjugates and FITC labeled AFP antibody was performed on Sephacryl S200 HR (separation range for globular proteins: 5 × 10 3 –2.5 × 10 5 Da) columns with different scales: 8 × 300 mm, 10 × 150 mm and 13 × 50 mm. Generally, the separation efficiency increases linearly with the length to diameter ratio of the column . The result obtained (as shown in Fig.…”

Section: Resultsmentioning

confidence: 77%

Size exclusion chromatography as a universal method for the purification of quantum dots bioconjugates

et al. 2013

View full text Add to dashboard Cite

The bioconjugation of fluorescent quantum dots (QDs) and purification of QDs bioconjugates are of vital importance in bioapplications. In this paper, we systematically investigated the purification efficiency of QDs bioconjugates and their stability during separation process by using size exclusion chromatography (SEC) technique, fluorescence spectroscopy, and fluorescence correlation spectroscopy (FCS). In this study, commercial QDs and fluorescein isothiocyanate (FITC) were used as labeling probes, and bovine serum albumin (BSA) and antibody (Erbitux) were used as mode samples. The covalently linkage and the electrostatic interaction were used in bioconjugation procedures. We systematically studied the effects of certain factors such as the scales of column, loading volume, elution buffer, and separation media on the purification efficiency of QDs bioconjugates by a home-built SEC device. And highly pure and monodisperse QDs bioconjugates could be obtained by SEC purification twice under the optimized conditions. Furthermore, we investigated the stability of QDs bioconjugates in different conjugation ways and purification conditions by FCS, and found that the stability of bioconjugates were poor when electrostatic binding mode was used. We also observed that Sephacryl S300 HR (separation range for globular proteins: 1 × 10(4) -1.5 × 10(6) Da) was the best choice for purifying the vast majority of QDs-bioconjugates. Our work described here will be constructive to popularization and applications of QDs in life science.

show abstract

Section: Resultsmentioning

confidence: 77%

Size exclusion chromatography as a universal method for the purification of quantum dots bioconjugates

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The experimental setup for an optically gated introduction is shown in Figure (a). The optically gated introduction is based on the photo bleaching of analytes, which decompose efficiently under the irradiation of a strong laser light . Gating and probe laser beams are focused onto a separation capillary from different positions that maintain a constant distance from each other.…”

Section: Hadamard Transform Cementioning

confidence: 99%

Laser‐Induced Fluorometry for Capillary Electrophoresis

Kaneta

2018

The Chemical Record

View full text Add to dashboard Cite

Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.

show abstract

“…As an alterative to traditional electrokinetic injection, optically-gated sample introduction was first developed for LC in small diameter capillaries [86] and for CE [87,88] to perform ultrafast separations. This injection method was utilized on a microchip beginning in 2000 [89].…”

Section: Optical Gating On a Microchipmentioning

confidence: 99%

Sample introduction techniques for microfabricated separation devices

Roddy

Ewing

2004

Electrophoresis

View full text Add to dashboard Cite

A great deal of progress has been made toward the development of the micro total analysis system (micro-TAS) since its inception in 1990. A wide variety of applications, including genomics, proteomics and drug discovery, have prompted the development of analytical methods capable of very high throughput while maintaining low cost. The micro-TAS concept addresses both of these requirements. Electrophoresis has been a key element in the development of the micro-TAS. Most chemical and biochemical assays utilize a separation component at some point during analysis. Genomics, in particular, depends almost exclusively on electrophoresis for size-based separations of DNA. This review examines sample introduction into microfabricated electrophoretic devices, or chips, primarily for DNA analysis. Sample introduction is an important component of these systems and is an essential process for making chip electrophoresis a widely applicable analytical technique. Specific issues, such as automation, the delivery of large numbers of samples to microfabricated devices and injection of picoliter-sized sample plugs into a separation lanes on chips, are presented.

show abstract

Sample gating in open tubular and packed capillaries for high-speed liquid chromatography

Cited by 45 publications

References 7 publications

Size exclusion chromatography as a universal method for the purification of quantum dots bioconjugates

Size exclusion chromatography as a universal method for the purification of quantum dots bioconjugates

Laser‐Induced Fluorometry for Capillary Electrophoresis

Sample introduction techniques for microfabricated separation devices

Contact Info

Product

Resources

About