Sample enrichment in a single levitated droplet for capillary electrophoresis

Petersson, Maria; Nilsson, Johan; Wallman, Lars; Laurell, Thomas; Johansson, Jonas; Nilsson, Staffan

doi:10.1016/s0378-4347(98)00092-9

Cited by 62 publications

(51 citation statements)

References 14 publications

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“…A basic analytical system (Fig. 2) consisting of an acoustic levitator combined with flow-through pL dispensers [14,44,45] has been used in our laboratory for bioanalytical purposes. This airborne analytical system [15,39,40,41,42] is equipped with different and exchangeable remote detection systems.…”

Section: Airborne Chemistrymentioning

confidence: 99%

“…The levitated drops are normally in the 100 nL-2 µL volume range and additions to the levitated drop can be made in the pL-volume range. It has been shown that the airborne system can be successfully used for single-cell analysis, [15,39] crystallisation studies of pharmaceuticals [46] and proteins [47] as well as for sample enrichment prior to capillary electrophoresis/capillary electrochromatography (CE/CEC) analysis [14]. For the single-cell experiments, a buffer drop containing living cells is levitated and different substances are presented to the cells using the pL flow-through dispensers.…”

Section: Airborne Chemistrymentioning

confidence: 99%

“…Operating at increased ultrasonic power during the positioning also enhances drop detachment. After positioning the levitated drop may perform oscillations, but it can be readily stabilised by adjusting the distance between the transducer and the reflector and by setting the ultrasonic power properly [14,15].…”

Section: Sample Handlingmentioning

confidence: 99%

“…Among the most serious drawbacks is the risk of analyte adsorption to walls and interfaces [12,13,14,15,16,17], a problem multiplied in small-dimensional analytic systems where volumes in the picolitre (pL) or nanolitre (nL) range are used. Another problem is optical interference at the walls of the sample container hampering detection [15], especially since such low amounts of analytes are being used.…”

Section: Introductionmentioning

confidence: 99%

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Airborne chemistry: acoustic levitation in chemical analysis

Santesson

Nilsson

2004

Analytical and Bioanalytical Chemistry

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216

116

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This review with 60 references describes a unique path to miniaturisation, that is, the use of acoustic levitation in analytical and bioanalytical chemistry applications. Levitation of small volumes of sample by means of a levitation technique can be used as a way to avoid solid walls around the sample, thus circumventing the main problem of miniaturisation, the unfavourable surface-to-volume ratio. Different techniques for sample levitation have been developed and improved. Of the levitation techniques described, acoustic or ultrasonic levitation fulfils all requirements for analytical chemistry applications. This technique has previously been used to study properties of molten materials and the equilibrium shape()and stability of liquid drops. Temperature and mass transfer in levitated drops have also been described, as have crystallisation and microgravity applications. The airborne analytical system described here is equipped with different and exchangeable remote detection systems. The levitated drops are normally in the 100 nL-2 microL volume range and additions to the levitated drop can be made in the pL-volume range. The use of levitated drops in analytical and bioanalytical chemistry offers several benefits. Several remote detection systems are compatible with acoustic levitation, including fluorescence imaging detection, right angle light scattering, Raman spectroscopy, and X-ray diffraction. Applications include liquid/liquid extractions, solvent exchange, analyte enrichment, single-cell analysis, cell-cell communication studies, precipitation screening of proteins to establish nucleation conditions, and crystallisation of proteins and pharmaceuticals.

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Section: Airborne Chemistrymentioning

confidence: 99%

Section: Airborne Chemistrymentioning

confidence: 99%

Section: Sample Handlingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Airborne chemistry: acoustic levitation in chemical analysis

Santesson

Nilsson

2004

Analytical and Bioanalytical Chemistry

Self Cite

216

116

View full text Add to dashboard Cite

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“…[8][9][10][11] Some ink-jet type dispensings have been used in many areas of chemistry and science, including the fabrication of biosensors, 12,13 sample introduction for mass spectrometers, 14,15 and sample introduction for capillary electrophoresis (CE). 16,17 In our previous studies, an ink-jet microchip was used for the introduction of nano-droplet samples in a thermal-conductivity detector (TCD), 18 a laboratory-made atomic emission detector (AED) 19 and the interface between liquid flow and a flame ionization detector (FID). 20 Some advantages of the small-sized ink-jet microchip as an injection device of analytical instruments are considered to be its easy set up, easy control of the number and volume of the injection by changing the electric parameters (piezoelectricdriven voltage, frequency and pulse wavelength, etc.).…”

Section: Introductionmentioning

confidence: 99%