Sample Collection and Preparation of Biofluids and Extracts for Liquid Chromatography-Mass Spectrometry

Yin, Peiyuan; Zhou, Lina; Zhao, Xinjie; Xu, Guowang

doi:10.1007/978-1-4939-2377-9_5

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…Fifty milligrams of freeze-dried liver power were weighed for each sample (placed in ice water until the next step). Metabolite extraction was carried out using 1.5 mL of methanol/water solution (methanol:water = 4:1, vol:vol) in the presence of a 5 mm diameter stainless steel bead using a homogenizer (Spex Sample PREP, Stanmore, UK) operating at 20 Hz for 2 min and stood for 60 min at −20 °C ( Yin et al., 2015 ). The mixture was then centrifuged at 30,000 × g for 3 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

The crucial role of lysine in the hepatic metabolism of growing Holstein dairy heifers as revealed by LC-MS-based untargeted metabolomics

Kong

Diao

et al. 2021

Animal Nutrition

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The objective of this experiment was to evaluate the effect of supplementing rumen-protected Lys based on a Lys-deficient diet on liver metabolism in growing Holstein heifers. The experiment was conducted for 3 months with 36 Holstein heifers (initial body weight: 200 ± 9.0 kg; 7-month-old). Heifers were randomly assigned to 2 diets based on corn, soybean meal, alfalfa hay, and wheat bran: control, Lys-deficient diet (LD; 0.66% Lys in diet), and Lys-adequate diet (LA; 1.00% Lys in diet). The results showed no difference in growth performance between the 2 groups ( P > 0.05). However, there was a clear trend of increasing feed conversion rate with Lys supplementation (0.05 < P < 0.01). The serum urea nitrogen concentration was significantly decreased, and the aspartate aminotransferase-to-alanine aminotransferase ratio was significantly decreased by Lys supplementation ( P < 0.05). Moreover, growing heifers fed a Lys-adequate diet had lower levels of urine nitrogen excretion and higher levels of the biological value of nitrogen ( P < 0.05). Metabolomic analysis revealed that 5 types of phosphatidylcholine and 3 types of ceramide were significantly increased and enriched in sphingolipid metabolism and glycerophospholipid metabolism ( P < 0.05). His, Leu, and Asp levels were significantly decreased in the liver following Lys supplementation ( P < 0.05). In conclusion, Lys supplementation may promote the synthesis of body tissue proteins, as evidenced by significantly decreased amino acids in the liver and urine N excretion, it also improves hepatic lipid metabolism by providing lipoprotein precursors.

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Section: Methodsmentioning

confidence: 99%

The crucial role of lysine in the hepatic metabolism of growing Holstein dairy heifers as revealed by LC-MS-based untargeted metabolomics

Kong

Diao

et al. 2021

Animal Nutrition

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Metabolomics of lung cancer: Analytical platforms and their applications

Noreldeen

Liu

2019

J of Separation Science

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Lung cancer is the leading type of cancer worldwide in terms of the number of new cases and is responsible for the largest number of deaths due to poor prognosis and difficult early detection. Due to its ability to detect numerous small molecular metabolites simultaneously, metabolomics has been widely used for the assessment of global metabolic changes in a living organism to discover candidate biomarkers for cancer diagnosis, investigate the development of cancer, and provide insights into the underlying pathophysiology. This review will mainly describe recent developments in lung cancer metabolomics in terms of early‐stage detection, biomarker discovery and mechanism exploration by using nuclear magnetic resonance, liquid chromatography–mass spectrometry, gas chromatography–mass spectrometry, and capillary electrophoresis–mass spectrometry in the last 10 years. The sample collection and metabolite extraction methods are also summarized.

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Metabolomics Toward Biomarker Discovery

Yin

2017

Methods in Molecular Biology

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Metabolomics has been used as practical tool in the discovery of novel biomarkers in a broad area in the clinic. The analytical platforms including nuclear magnetic resonance (NMR) and mass spectrometry (MS) can cover thousands of metabolites. With the help of multivariate data analysis, many potential biomarkers can be defined in the studies. Since metabolites stand at the end point of metabolism, it remains difficult to find novel biomarkers with good diagnostic or prognostic performance. In this chapter, we will introduce a general protocol for biomarker discovery within the scope of metabolomics using MS.

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Sample Collection and Preparation of Biofluids and Extracts for Liquid Chromatography-Mass Spectrometry

Cited by 6 publications

References 26 publications

The crucial role of lysine in the hepatic metabolism of growing Holstein dairy heifers as revealed by LC-MS-based untargeted metabolomics

The crucial role of lysine in the hepatic metabolism of growing Holstein dairy heifers as revealed by LC-MS-based untargeted metabolomics

Metabolomics of lung cancer: Analytical platforms and their applications

Metabolomics Toward Biomarker Discovery

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