SAMP14, a Novel, Acrosomal Membrane-associated, Glycosylphosphatidylinositol-anchored Member of the Ly-6/Urokinase-type Plasminogen Activator Receptor Superfamily with a Role in Sperm-Egg Interaction

Shetty, Jagathpala; Wolkowicz, Michael J.; Digilio, Laura; Klotz, Kenneth L.; Jayes, Friederike L.; Diekman, Alan B.; Westbrook, V. Anne; Farris, Erin M.; Hao, Zhonglin; Coonrod, Scott A.; Flickinger, Charles J.; Herr, John C.

doi:10.1074/jbc.m301713200

Cited by 100 publications

(69 citation statements)

References 41 publications

(24 reference statements)

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“…35,[56][57][58] SPESP1 was one of the candidates functioning for fusion, because anti-SPESP1 antibody was known to inhibit human sperm from fusing with hamster eggs as well as inhibiting sperm-egg fusion in mouse. 59 A mouse line lacking Spesp1 was produced by homologous recombination and the fertilizing ability of male mice was examined.…”

Section: Gamete Interaction N Inoue Et Al 84mentioning

confidence: 99%

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

Inoue¹,

Ikawa²,

Okabe³

2010

Asian J Androl

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An average human ejaculate contains over 100 million sperm, but only a few succeed in accomplishing the journey to an egg by migration through the female reproductive tract. Among these few sperm, only one participates in fertilization. There might be an ingenious molecular mechanism to ensure that the very best sperm fertilize an egg. However, recent gene disruption experiments in mice have revealed that many factors previously described as important for fertilization are largely dispensable. One could argue that the fertilization mechanism is made robust against gene disruptions. However, this is not likely, as there are already six different gene-disrupted mouse lines (Calmegin, Adam1a, Adam2, Adam3, Ace and Pgap1), all of which result in male sterility. The sperm from these animals are known to have defective zona-binding ability and at the same time lose oviduct-migrating ability. Concerning spermzona binding, the widely accepted involvement of sugar moiety on zona pellucida 3 (ZP3) is indicated to be dispensable by gene disruption experiments. Thus, the landscape of the mechanism of fertilization is revolving considerably. In the sperm-egg fusion process, CD9 on egg and IZUMO1 on sperm have emerged as essential factors. This review focuses on the mechanism of fertilization elucidated by gene-manipulated animals.

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Section: Gamete Interaction N Inoue Et Al 84mentioning

confidence: 99%

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

Inoue¹,

Ikawa²,

Okabe³

2010

Asian J Androl

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“…Beyond the more massive secondary zona binding (compared to the primary zona recognition binding at the sperm surface), the exposed intra-acrosomal proteins also cause a local enzymatic cleavage of this network of 3-4 heavily glycosylated zona proteins [66,[69][70][71][72][73][74][75]. For a review on secondary zona binding proteins from the acrosome see [7].…”

Section: Zona Penetration After the Acrosome Reactionmentioning

confidence: 99%

Membrane Fusions During Mammalian Fertilization

Gadella

Evans

2011

Advances in Experimental Medicine and Biology

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Successful completion of fertilization in mammals requires three different types of membrane fusion events. Firstly, the sperm cell will need to secrete its acrosome contents (acrosome exocytosis; also known as the acrosome reaction); this allows the sperm to penetrate the extracellular matrix of the oocyte (zona pellucida) and to reach the oocyte plasma membrane, the site of fertilization. Next the sperm cell will bind and fuse with the oocyte plasma membrane (also known as the oolemma), which is a different type of fusion in which two different cells fuse together. Finally, the fertilized oocyte needs to prevent polyspermic fertilization, or fertilization by more than one sperm. To this end, the oocyte secretes the contents of cortical granules by exocytotic fusions of these vesicles with the oocyte plasma membrane over the entire oocyte cell surface (also known as the cortical reaction or cortical granule exocytosis). The secreted cortical contents modify the zona pellucida, converting it to a state that is unreceptive to sperm, constituting a block to polyspermy. In addition, there is a block at the level of the oolemma (also known as the membrane block to polyspermy). IntroductionFertilization of the oocyte involves three membrane fusion events [1] namely, (1) a preparative series of secretion membrane fusions at the apical sperm surface known as acrosome exocytosis [2]. The membrane fusions are induced when the sperm cell binds to specific zona binding proteins at the sperm surface [3][4][5][6][7]. The acrosome exocytosis is a multipoint membrane fusion event between the sperm plasma membrane and the outer acrosomal membrane (see Fig. 5.1 [8,9]) and the exposed acrosomal content is required for sperm to penetrate the zona pellucida [10][11][12]. This so-called zona drilling effectively takes place because the sperm at this stage also has acquired hyperactivated motility [13]. (2) After zona penetration the sperm enters the perivitelline space where it can bind and fuse with the oocyte plasma membrane [14,15]. This is the actual fertilization fusion in which the contents of the sperm are delivered into the oocyte cytoplasm. The plasma membrane of the equatorial segment (see Fig. 5.1) is the site where proteins are located that orchestrate sperm-oocyte binding and fusion [16]. (3) In order to prevent polyspermy the oocyte has to activate defense systems to block redundant sperm-oocyte fusion [17]. To this end the first fertilizing sperm delivers activation factors

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“…Of the five antigens selected for this study, four are associated with the acrosome both before and after the acrosome reaction (AR): ESP, SAMP 32, SAMP 14 and SP-10 (Herr et al, 1990a,b;Kurth et al, 1991;Foster and Herr, 1992;Foster et al, 1994;Wolkowicz et al, 1996Wolkowicz et al, , 2003Hao et al, 2002;Shetty et al, 2003). The fifth antigen, SLLP-1, is found in the acrosomal matrix before the AR but its location after the AR is unclear .…”

Section: Introductionmentioning

confidence: 99%

“…The fifth antigen, SLLP-1, is found in the acrosomal matrix before the AR but its location after the AR is unclear . Other criteria for selection were testis-specificity, postmeiotic expression, iso-antigenicity and the proven ability of antibodies or recombinant proteins to block sperm-egg binding and/or fusion in vitro in the xenoassay using human sperm and hamster eggs (hamster egg penetration test,HEPT) (Kurth et al, 1991;Coonrod et al, 1996;Wolkowicz et al, 1996Wolkowicz et al, , 2003Hao et al, 2002;Mandal et al, 2003;Shetty et al, 2003). Thus, antibodies to all the human immunogens chosen for this study have provided evidence for inhibitory effects on fertilization in vitro.…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis

Kurth

Digilio

Snow

et al. 2008

Journal of Reproductive Immunology

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A vaccine formula comprised of five recombinant human intra-acrosomal sperm proteins was innoculated into female monkeys to test whether specific antibodies to each component immunogen could be elicited in sera and whether antibodies elicited by the vaccine affected in vitro fertilization. Acrosomal proteins, ESP, SLLP-1, SAMP 32, SP-10 and SAMP 14, were expressed with his-tags, purified by nickel affinity chromatography and adsorbed to aluminum hydroxide. Five female cynomolgus monkeys were inoculated intramuscularly 3 times at monthly intervals. All five monkeys developed both IgG and IgA serum responses to each recombinant immunogen on Western blots. Each serum stained the acrosome of human sperm and bound to the cognate native protein on Western blots of human sperm extracts. By ELISA, all monkeys developed IgG to each immunogen, with the highest average absorbance values to ESP, SAMP 32 and SP-10, followed by lower values for SLLP-1 and SAMP 14. IgA was also generated to each component immunogen with the highest average absorbance values to SLLP-1 and SP-10. For antigens that induced an IgA response, the duration of the IgA response was longer than the IgG response to the same antigens. This study supports the concept that a multivalent contraceptive vaccine may be administered to female primates evoking both peripheral (IgG) and mucosal (IgA) responses to each component immunogen following an intramuscular route of inoculation with a mild adjuvant, aluminum hydroxide, approved for human use.

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SAMP14, a Novel, Acrosomal Membrane-associated, Glycosylphosphatidylinositol-anchored Member of the Ly-6/Urokinase-type Plasminogen Activator Receptor Superfamily with a Role in Sperm-Egg Interaction

Cited by 100 publications

References 41 publications

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

The mechanism of sperm–egg interaction and the involvement of IZUMO1 in fusion

Membrane Fusions During Mammalian Fertilization

Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis

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