Salvage pathway for NAD biosynthesis in Brevibacterium ammoniagenes: regulatory properties of triphosphate-dependent nicotinate phosphoribosyltransferase

Dulyaninova, Natalya G.; Podlepa, E. M.; Toulokhonova, Larisa; Bykhovsky, Vladimir Ya.

doi:10.1016/s0167-4838(00)00045-5

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…Thus, availability of ATP as well as PRPP regulates the formation of NaMN. PuriWcation and characterisation of plant NaPRT has not yet been performed, but feedback inhibition by NAD of NaPRT puriWed from Brevibacterium has been reported (Dulyaninova et al 2000). In the case of PNC VI, NaR may be a key metabolite.…”

Section: Discussionmentioning

confidence: 99%

Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.)

Katahira

Ashihara

2009

Planta

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As part of a research program on nucleotide metabolism in potato tubers (Solanum tuberosum L.), profiles of pyridine (nicotinamide) metabolism were examined based on the in situ metabolic fate of radio-labelled precursors and the in vitro activities of enzymes. In potato tubers, [(3)H]quinolinic acid, which is an intermediate of de novo pyridine nucleotide synthesis, and [(14)C]nicotinamide, a catabolite of NAD, were utilised for pyridine nucleotide synthesis. The in situ tracer experiments and in vitro enzyme assays suggest the operation of multiple pyridine nucleotide cycles. In addition to the previously proposed cycle consisting of seven metabolites, we found a new cycle that includes newly discovered nicotinamide riboside deaminase which is also functional in potato tubers. This cycle bypasses nicotinamide and nicotinic acid; it is NAD --> nicotinamide mononucleotide --> nicotinamide riboside --> nicotinic acid riboside --> nicotinic acid mononucleotide --> nicotinic acid adenine dinucleotide --> NAD. Degradation of the pyridine ring was extremely low in potato tubers. Nicotinic acid glucoside is formed from nicotinic acid in potato tubers. Comparative studies of [carboxyl-(14)C]nicotinic acid metabolism indicate that nicotinic acid is converted to nicotinic acid glucoside in all organs of potato plants. Trigonelline synthesis from [carboxyl-(14)C]nicotinic acid was also found. Conversion was greater in green parts of plants, such as leaves and stem, than in underground parts of potato plants. Nicotinic acid utilised for the biosynthesis of these conjugates seems to be derived not only from the pyridine nucleotide cycle, but also from the de novo synthesis of nicotinic acid mononucleotide.

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Section: Discussionmentioning

confidence: 99%

Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.)

Katahira

Ashihara

2009

Planta

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“…Interestingly, we did find strong induction of the enzyme nicotine-phosphoribosyl transferase (spot. 417) too, which catalyzes the first reaction in the synthesis of NAD ( Dulyaninova et al, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Psychrophilic vs. Mesophilic Bacterial Species Reveals Different Strategies to Achieve Temperature Adaptation

2022

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The old debate of nature (genes) vs. nurture (environmental variables) is once again topical concerning the effect of climate change on environmental microorganisms. Specifically, the Polar Regions are experiencing a drastic increase in temperature caused by the rise in greenhouse gas emissions. This study, in an attempt to mimic the molecular adaptation of polar microorganisms, combines proteomic approaches with a classical microbiological analysis in three bacterial species Shewanella oneidensis, Shewanella frigidimarina, and Psychrobacter frigidicola. Both shewanellas are members of the same genus but they live in different environments. On the other hand, Shewanella frigidimarina and Psychrobacter frigidicola share the same natural environment but belong to a different genus. The comparison of the strategies employed by each bacterial species estimates the contribution of genome vs. environmental variables in the adaptation to temperature. The results show a greater versatility of acclimatization for the genus Shewanella with respect to Psychrobacter. Besides, S. frigidimarina was the best-adapted species to thermal variations in the temperature range 4–30°C and displayed several adaptation mechanisms common with the other two species. Regarding the molecular machinery used by these bacteria to face the consequences of temperature changes, chaperones have a pivoting role. They form complexes with other proteins in the response to the environment, establishing cooperation with transmembrane proteins, elongation factors, and proteins for protection against oxidative damage.

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“…Thus, availability of ATP as well as PRPP regulates the formation of NaMN. Purification and characterisation of plant NaPRT has not yet been performed, but feedback inhibition by NAD of NaPRT purified from Brevibacterium has been reported (Dulyaninova et al, 2000). In contrast, other steps including nicotinamidase and NaMN adenylyltransferase appear not to be the rate-limiting steps on the PNC, since there was little accumulation of the substrates nicotinamide and NaMN found in most plants (Matsui et al, 2007;Katahira and Ashihara, 2009).…”

Section: Pyridine Nucleotide Cyclementioning

confidence: 99%

Nucleotide Metabolism

Zrenner¹,

Ashihara²

2011

Plant Metabolism and Biotechnology

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Salvage pathway for NAD biosynthesis in Brevibacterium ammoniagenes: regulatory properties of triphosphate-dependent nicotinate phosphoribosyltransferase

Cited by 13 publications

References 19 publications

Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.)

Profiles of the biosynthesis and metabolism of pyridine nucleotides in potatoes (Solanum tuberosum L.)

Comparative Proteomic Analysis of Psychrophilic vs. Mesophilic Bacterial Species Reveals Different Strategies to Achieve Temperature Adaptation

Nucleotide Metabolism

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