Salvadora persica Extract-laden Jellyfish Collagen Hybrid Constructs for Periodontal Tissue Regeneration

Abstract

Considerable effort in the field of periodontal tissue engineering has been expended in the construction of advanced biomatrix for the treatment of periodontal diseases caused by poor oral hygiene, malnutrition, genetic factors, and systemic disorders. With these in mind, the ultimate goal of this investigation is to fabricate sophisticated scaffolds using jellyfish collagen (JC) and aqueous Salvadora persica (Miswak) extracts. Rhizostoma pulmo species JC was isolated and characterized in depth. Miswak was extracted using two different methods. The extraction yield was calculated to be 14.2 ± 0.9% and 17.1 ± 0.4% for Methods I and II, respectively. Gas chromatography-mass spectroscopy (GC-MS) results revealed the extract to be composed of 1,8-cineole (49.3%), benzyl nitrile (36.2%), benzyl isothiocyanate (5.9%), limonene (2.4%), eugenol (0.8%), and palmitic acid (0.3%). Total phenolic content and antioxidant capacities of the extracts were also determined by spectrophotometric means. Human periodontal ligament fibroblast cells were isolated and expanded. Cell viability on JC and miswak extract-laden JC scaffolds was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay. Microarchitectures of the JC, 0.05 and 0.1% miswak extract-laden JC scaffolds and also cellular behaviors on these surfaces were evaluated by scanning electron microscopy (SEM) analysis. This study suggests that miswak extract-laden JC scaffolds would present new opportunities for periodontal tissue engineering.

HPLC/MSn Profiling and Healing Activity of a Muco-Adhesive Formula of Salvadora persica against Acetic Acid-Induced Oral Ulcer in Rats

“…In accordance with these findings, S. persica extract has been reported to inhibit collagen degradation in demineralised dentin [ 71 ]. Moreover, S. persica extract-laden collagen hybrid constructs showed enhanced periodontal tissue regeneration [ 72 ], consistent with the ability of S. persica to enhance fibroblast proliferation and viability [ 73 ]. This also supports the observed ability of S. persica to enhance angiogenesis, as evidenced by increased Ang-1 mRNA expression.…”

Synthesis of Silica-Based Boron-Incorporated Collagen/Human Hair Keratin Hybrid Cryogels with the Potential Bone Formation Capability

“…The resulting mixture was poured into a 48-well PTFE mold, frozen immediately at −80 °C, and freeze-dried overnight. To increase the mechanical strength of the obtained bioscaffolds, a crosslinking procedure was included . Briefly, MES buffer (0.05 M, pH 5.5) was mixed with ethanol (99%) at a ratio of 1:9 (v/v) to prepare an activation/coupling buffer.…”

Exploring the Therapeutic Potentials of Exopolysaccharides Derived From Lactic Acid Bacteria and Bifidobacteria: Antioxidant, Antitumor, and Periodontal Regeneration

“…Human periodontal ligament fibroblast cells (hPDLFCs) were extracted and grown according to Arslan and Kantarcioglu (2019) . The Clinical Study Ethics Committee of Taif University accepted the research on an ethical basis.…”