Salusin-β, an Antimicrobially Active Peptide against Gram-Positive Bacteria

Kimura, Masahiro; Shindo, Mitsuno; Moriizumi, Toshiyuki; Tagawa, Noriko; Fujinami, Aya; Kato, Ikuo; Uchida, Yoshiki

doi:10.1248/cpb.c14-00103

Cited by 6 publications

(6 citation statements)

References 13 publications

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Section: Discussionmentioning

confidence: 99%

“…Salusin-β had the ability to depolarize the cytoplasmic membrane, and this may be linked to the antibacterial activity of this agent. 22 However, the relationship of salusin-β with sepsis or the function of salusin-β in inflammation is still unclear. This study clearly indicated that the serum and tissues of rat contain a certain amount of salusin-β, and the level of spleen tissue was the highest, followed by the hypothalamus, small intestine, and stomach tissues, which were the lowest.…”

Section: Discussionmentioning

confidence: 99%

“…Salusin-β had the ability to depolarize the cytoplasmic membrane, and this may be linked to the antibacterial activity of this agent. 22 However, little information is available yet on the relationships of salusin-β with sepsis. Based on the above studies, we hypothesized that salusins might be involved in the pathogenesis of inflammatory disease, including sepsis.…”

Section: The Time Changes Of Endogenous Salusin-β In Septic Ratsmentioning

confidence: 99%

“…Salusin-β had the ability to depolarize the cytoplasmic membrane, and this may be linked to the antibacterial activity of this agent. 22…”

Section: Introductionmentioning

confidence: 99%

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The time changes of endogenous salusin-β in septic rats

Xie

Chai

et al. 2017

Eur J Inflamm

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Salusin-α and salusin-β are newly found bioactive peptides of 28 and 20 amino acids, respectively, which are widely distributed in the hematopoietic system, endocrine system, and central nervous system. Salusins exert cardiovascular effects, including hypotension and bradycardia; promote vascular inflammation; and so on. However, little information is available yet on the relationships of salusin-β with sepsis. In this study, we investigated the distribution and content of endogenous salusin-β in septic rats. A total of 72 specified pathogen-free (SPF) male Sprague-Dawley (SD) rats were randomly divided into control group (sham operation, n = 36) and experimental group (n = 36) with sepsis rat model by cecal ligation and puncture (CLP). The model group rats were sacrificed after 6, 12, and 24 h of treatment. The concentration of salusin-β in spleen, stomach, small intestine, hypothalamus, and serum specimens was detected by enzyme-linked immunosorbent assay (ELISA). It showed that salusin-β was endogenously generated in rat tissues, including spleen, stomach, small intestine, hypothalamus, and serum. The content of salusin-β in the spleen was higher than that in other tissues. The content of salusin-β in the spleen, stomach, and small intestine, together with the serum level of salusin-β, increased significantly at 6 h after CLP compared with the control group (P < 0.05). The content of salusin-β in spleen and serum peaked at 12 h, and in small intestine, it reached the summit at 24 h. Meanwhile, no significant fluctuations in salusin-β content were observed in the stomach. The content of salusin-β in hypothalamus began to increase at 6 h, and a significant increase appeared 12 h after CLP (P < 0.05). In conclusion, this study shows that the time-dependent alterations of salusin-β in septic rats suggest that salusin-β might be involved in the pathogenesis of sepsis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Time Changes Of Endogenous Salusin-β In Septic Ratsmentioning

confidence: 99%

“…Salusin-β had the ability to depolarize the cytoplasmic membrane, and this may be linked to the antibacterial activity of this agent. 22…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The time changes of endogenous salusin-β in septic rats

Xie

Chai

et al. 2017

Eur J Inflamm

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“…[1][2][3] Several studies have demonstrated that these peptides are associated with conditions such as hypertension, atherosclerotic cardiovascular disease, acute coronary syndrome, and vascular resistance. 1,4) In a previous study, 5) we found that salusin-β exhibits antibacterial activity against Gram-positive microorganisms, such as Staphylococcus aureus NBRC 12732, but had no activity against Gram-negative bacteria. We also reported that salusin-β depolarizes the bacterial cytoplasmic membrane of S. aureus NBRC 12732, which may be linked to its antibacterial activity.…”

Section: Introductionmentioning

confidence: 89%

Potent Antibacterial Activity of Synthetic Peptides Designed from Salusin-β and HIV-1 Tat(49–57)

Kimura

Kosuge

et al. 2020

CHEMICAL & PHARMACEUTICAL BULLETIN

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Salusin-β is an endogenous bioactive peptide that was identified in a human full-length enriched cDNA library using bioinformatics analyses. In our previous study, we found that synthetic salusin-β exhibits antibacterial activity against only Gram-positive microorganisms such as Staphylococcus aureus NBRC 12732. Salusin-β has an ability to depolarize the cytoplasmic membrane of this bacterium, and this phenomenon may be linked to the antibacterial activity of this peptide. A cell-penetrating peptide (CPP), human immunodeficiency virus (HIV)-1 transactivator of transcription (Tat) (49-57) is a short cationic peptide that can traverse cell membranes. In this report, synthetic peptide conjugates of salusin-β and HIV-1 Tat(49-57) showed potent antibacterial activities against both Gram-positive Staphylococcus aureus NBRC 12732 and Gram-negative Escherichia coli NBRC 12734. The synthetic peptides also depolarized the cytoplasmic membrane of Escherichia coli NBRC 12734 as well as Staphylococcus aureus NBRC 12732. These results suggested that HIV-1 Tat(49-57) is a protein transduction domain or CPP that changes the interaction mode between salusin-β and the cell membrane of Escherichia coli NBRC 12734. By binding to HIV-1 Tat(49-57), salusin-β showed a broad antibacterial spectrum regardless of whether the target was a Gram-positive or Gram-negative bacterium.

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Gestational Diabetes and Peptides in Breast Milk

Aydın

2017

Nutrition and Diet in Maternal Diabetes

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Salusin-β, an Antimicrobially Active Peptide against Gram-Positive Bacteria

Abstract: Salusin-β has been detected in numerous mammalian tissues and has been shown to have various effects on the cardiovascular system. In this study, we showed that salusin-β exhibited potent antibacterial activity against Gram

Cited by 6 publications

References 13 publications

The time changes of endogenous salusin-β in septic rats

The time changes of endogenous salusin-β in septic rats

Potent Antibacterial Activity of Synthetic Peptides Designed from Salusin-β and HIV-1 Tat(49–57)

Gestational Diabetes and Peptides in Breast Milk

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