Salting‐out‐assisted liquid–liquid extraction with acetonitrile for the determination of trimetazidine in rat plasma using liquid chromatography–mass spectrometry

Xiong, Xin; Yang, Li

doi:10.1002/bmc.3271

Cited by 13 publications

(10 citation statements)

References 18 publications

(21 reference statements)

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“…Sample preparation is a key step in analyzing small molecules from biological fluids owing to the presence of endogenous matrix components. The literature presents a variety of extraction procedures for TMZ, including protein precipitation (PP) (Jiao et al, ; Medvedovici et al, ; Xiong et al, ; Zhou et al, ; Zou et al, ), hollow fiber‐based liquid phase microextraction (Lv et al, ), liquid–liquid extraction (LLE) (Chowdhury et al, ; Ding et al, ; Grabowski et al, ; Helmy & Mansour, ; Ozbay et al, ; Wang et al, ; Zhang et al, ), LLE followed by precolumn derivatization (de Jager et al, ; Khedr et al, ), salting‐out‐assisted LLE (Xiong & Yang, ) and SPE (Mistri et al, ). The majority of the methods have utilized LLE for selective extraction of TMZ from human plasma; however, the recovery obtained using different solvent systems was in the range of 57–75%.…”

Section: Resultsmentioning

confidence: 99%

“…Several methods are reported for determination of TMZ in different biological samples such as human plasma (de Jager et al, ; Ding, Gong, Chu, Hu, & Zheng, ; Grabowski et al, ; Helmy & Mansour, ; Jiao et al, ; Khedr, Sheha, & Darwish, ; Liu et al, ; Lv et al, ; Medvedovici, Albu, Georgiţ, & David, ; Mistri, Jangid, & Shrivastav, ; Ozbay, Unal, & Erol, ; Wang, Sun, Rong, Tang, & He, ; Zhang et al, ; Zhou et al, ; Zou, Dai, Wang, Zhu, & Fan, ), human serum (Chowdhury et al, ), rat plasma (Xiong & Yang, ; Xiong, Yang, & Zhai, ), and pig and dog plasma (Génissel, Chodjania, Demolis, Ragueneau, & Jaillon, ). Mainly, high‐performance liquid chromatography (HPLC) with fluorescence (Khedr et al, ), electrochemical (Grabowski et al, ) and UV (Chowdhury et al, ; Helmy & Mansour, ; Lv et al, ) detection, liquid chromatography–mass spectrometry (LC–MS) (de Jager et al, ; Ding et al, ; Jiao et al, ; Ozbay et al, ; Wang et al, ) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) (Liu et al, ; Medvedovici et al, ; Mistri et al, ; Xiong et al, ; Xiong & Yang, ; Zhou et al, ; Zou et al, ) have been the preferred techniques for quantification of TMZ in these matrices. In addition, a few other reports present the use of gas chromatography with nitrogen–phosphorous (Barre et al, ) and mass detection (Fay, Michel, Goupit, Harpey, & Prost, ).…”

Section: Introductionmentioning

confidence: 99%

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Overcoming interference of plasma phospholipids using HybridSPE for the determination of trimetazidine by UPLC–MS/MS

Vanol

Yadav

Sanyal

et al. 2017

Biomedical Chromatography

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An improved, precise and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the quantification of trimetazidine, using trimetazidine-d8 as the internal standard (IS). Interference owing to plasma phospholipids during sample preparation was overcome using a hybrid solid-phase extraction-phospholipid ultra cartridge. The mean extraction recovery of trimetazidine (98.66%) and trimetazidine-d8 (97.63%) from spiked plasma was consistent and reproducible. Chromatographic analysis was performed on a UPLC Ethylene Bridged Hybrid (BEH) C18 (50 × 2.1 mm, 1.7 μm) column with isocratic elution using acetonitrile-5 mm ammonium formate, pH 3.5 (40:60, v/v) as the mobile phase. The parent → product ion transitions for trimetazidine (m/z 267.1 → 181.1) and trimetazidine-d8 (m/z 275.2 → 181.1) were monitored on a triple quadrupole mass spectrometer with electrospray ionization functioning in the positive multiple reaction monitoring mode. The linearity of the method was established in the concentration range of 0.05-100 ng/mL for trimetazidine. The intra-batch and inter-batch accuracy and precision (CV) were 97.3-103.1 and 1.7-5.3%, respectively. Qualitative and quantitative assessment of matrix effect showed no interference of endogenous/exogenous components. The developed method was used to measure plasma trimetazidine concentration for a bioequivalence study with 12 healthy subjects.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overcoming interference of plasma phospholipids using HybridSPE for the determination of trimetazidine by UPLC–MS/MS

Vanol

Yadav

Sanyal

et al. 2017

Biomedical Chromatography

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“…Mostly used techniques for pretreatment of urine samples containing trimetazidine are liquid-liquid extraction (LLE) [14] and solid-phase extraction (SPE) [7,15,16]. Analogous procedures can be also found for plasma samples, where the puri cation step is even more complicated due to the presence of proteins, which needs to be salted out, and other compounds [5,6,10,17].…”

Section: Introductionmentioning

confidence: 99%

Determination of Trimetazidine in Urine by Capillary Electrophoresis with Amperometric Detection

Baroch

Dejmková

Matysik

2023

Preprint

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In this contribution nonaqueous capillary electrophoresis with end-column amperometric detection using a platinum ultramicroelectrode is presented along with several extraction procedures, both liquid-liquid extraction (LLE) and solid-phase extraction (SPE), for extracting Trimetazidine (TMZ) from urine. Trimetazidine is an anti-ischemic drug, which changes hearth metabolism pathways and is being abused as a doping. Electrochemical detection in a non-aqueous environment provided a stable response with a relative standard deviation of only 3.6% (n = 10) in repeatability measurement at concentration of 50 µg dm-3. LOD and LOQ of the proposed method were determined as 0.054 µg dm-3 and 0.180 µg dm-3, respectively. From the point of view of LLE, the most efficient procedure was the double extraction with ethyl acetate as an extraction agent in combination with prior alkalinization of the sample by Na2CO3. Nevertheless, the extraction efficiency was only around 68%. The most efficient SPE procedure was based on the combination of HLB cartridge and elution with background electrolyte containing 20% of methanol. Its recovery reached up to 92% and 101% in case of 50 µg dm-3 and 5.0 µg dm-3 of TMZ in urine, respectively.

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“…The method is simple, fast, cheap and environmentally friendly and its extract is also compatible with HPLC/LC, CE and/or GC instruments [36,37]. SALLE has been applied for extraction of various organic compounds including α-dicarbonyl compounds in beer [24] and wines [25]; biogenic amines in fruit juices and alcoholic beverages [26]; N-nitrosamines in skin care cosmetics [27]; trimetazidine in rat plasma [28]; methoxetamine in rat tissues (rat brain, liver and lungs) [29]; sulfonamides from different matrices (tea, water, milk, honey, human urine, plasma and blood) [30,31]; temozolomide from plasma [32]; vitamin K homologues in human plasma [33]; multi-mycotoxin biomarkers in pig urine [34]; betalactam antibiotics in infant dairy products [35]; benzimidazole fungicides [36]; sulfonylurea herbicides in water and banana juice samples [37]; triazole pesticides in water samples [38]; multiresidue pesticides in surface, ground and drinking water [39]; and chlorophenols in wine [40]. However, to date there is no report on the use of SALLE procedure in combination with HPLC-UV/Vis for analysis of multiclass pesticide residues in alcoholic beverages.…”

Section: Introductionmentioning

confidence: 99%

Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

Bedassa¹

2017

SJAC

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Abstract:A salting-out assisted liquid-liquid extraction (SALLE) method followed by high-performance liquid chromatography with ultraviolet-visible detector (HPLC-UV/Vis) has been proposed for determination of five multiclass pesticides residues including, atrazine, ametryn, terbutryn, carbaryl and chlorothalonil from various alcoholic beverages: beer, wine and Ethiopian honey wine (Tej). Experimental parameters influencing the extraction efficiency of the method such as the type and concentration of salt, volume of acetonitrile (the extraction solvent) and pH of the sample were assayed and the optimum conditions were established. Under the optimum experimental conditions, matrix-matched calibration curves were constructed using beer sample as the representative matrix and good linearity over wide concentration ranges were obtained with coefficient of determination (r 2 ) of 0.997 or better. The limits of detection (LOD) and quantification (LOQ) of the method, which were determined as 3 and 10 times a signal-to-noise ratio (S/N), were in the ranges of 1.3-3.9 and 4.5-12.8 µg L -1 , respectively. Precisions studied in terms of repeatability and intermediate precision (with-in lab reproducibility) at two concentration levels have demonstrated acceptable %RSD values, which were less than 10% in both cases. The applicability of the method was also investigated by analyzing various alcoholic beverages and demonstrated satisfactory recoveries in the ranges of 71-104% with their corresponding %RSDs less than 10% in all cases. The results of the study revealed that the developed SALLE method is selective and efficient sample preparation procedure prior to quantitative analysis of the target analytes by HPLC-UV/Vis.

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Salting‐out‐assisted liquid–liquid extraction with acetonitrile for the determination of trimetazidine in rat plasma using liquid chromatography–mass spectrometry

Cited by 13 publications

References 18 publications

Overcoming interference of plasma phospholipids using HybridSPE for the determination of trimetazidine by UPLC–MS/MS

Overcoming interference of plasma phospholipids using HybridSPE for the determination of trimetazidine by UPLC–MS/MS

Determination of Trimetazidine in Urine by Capillary Electrophoresis with Amperometric Detection

Salting-out Assisted Liquid-Liquid Extraction for the Determination of Multiresidue Pesticides in Alcoholic Beverages by High Performance Liquid Chromatography

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