Saltatory rolling circle amplification for sensitive visual detection of Staphylococcus aureus in milk

Yang, Qian; Zhang, Yunzhe; Li, Sen; Lü, Xin; Yuan, Yaowu; Zhang, Wei

doi:10.3168/jds.2019-16724

Cited by 33 publications

(18 citation statements)

References 24 publications

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“…In addition, the detection competence of the SRCA assay was greater than the recently developed Salmonella PSR assay with the detection limit of 4 9 10 3 and 4 CFU per gram without enrichment and after 6-h enrichment, respectively (Momin et al 2020). Previously published SRCA assays to detect Shigella in artificially spiked vegetable salad and S. aureus in milk reported LoDs of 47 CFU per gram and 56 CFU per ml, respectively without enrichment, which are in line with our study results (Wang et al 2018;Yang et al 2019). However, a recently published SRCA assay to detect Alicyclobacillus acidoterrestris in spiked apple juice could detect 7Á1 CFU per ml without enrichment (Yuan et al 2020).…”

Section: Discussionsupporting

confidence: 89%

“…Moreover, the current SRCA assay had a better analytical sensitivity than the polymerase spiral reaction (PSR) assay previously developed by our group for detecting Salmonella in pork (Momin et al 2020). However, a few previous SRCA assays reported about 10 times better analytical sensitivity than our current result (70 fg) (Wang et al 2018;Yang et al 2019). Limitations notwithstanding, our SRCA assay and all other SRCA assays till date revealed 100-fold higher sensitivity than conventional PCR and 10-fold higher sensitivity than real-time PCR assays (Wang et al 2018;Yang et al 2019;Zhang et al 2019;Yuan et al 2020;Milton et al 2021b).…”

Section: Discussioncontrasting

confidence: 69%

“…Owing to template-independent properties of Bst DNA polymerase, DNA gets synthesized at the intersection of non-closed circular DNA and continues to produce DNA at the primary primer binding location, simply like saltating a nick or skipping a gap (Zyrina et al 2007). Unlike RCA, SRCA does not require ligase and padlock probes to complete the cyclization (Wang et al 2018;Yang et al 2019;Zhang et al 2019;Yuan et al 2020) and requires just a single pair of simple PCR primers. The technique does not require thermocycler or gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

“…The technique does not require thermocycler or gel electrophoresis. To date, SRCA assays have been developed only for Shigella (Wang et al 2018), Staphylococcus aureus (Yang et al 2019), Cronobacter sp. (Zhang et al 2019), Alicyclobacillus acidoterrestris (Yuan et al 2020) and Clostridium perfringens (Milton et al 2021b).…”

Section: Introductionmentioning

confidence: 99%

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Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification

et al. 2021

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Aim:The aim of this study was to develop a saltatory rolling circle amplification (SRCA) assay for rapid, simple and visual detection of Salmonella in meat. Methods and Results: Saltatory rolling circle amplification assay was established using simple PCR primers targeting the invA gene of Salmonella enterica. The specificity of the SRCA assay was determined using 28 Salmonella and 15 non-Salmonella strains. The analytical sensitivity of the developed SRCA, conventional and real-time PCR assays were 70 fg, 7 pg and 700 fg S. enterica DNA per tube, respectively. The limit of detection (LoD) of the SRCA assay was 40 CFU per gram of meat without enrichment and 4 CFU per gram after including 6 h brief enrichment step. The detection limits of 40 CFU per gram and 4 CFU per gram of meat were achieved within 165 min and 9 h, respectively (including DNA extraction). To assess the real-world relevance of the SRCA assay, it was used to screen Salmonella from the field pork samples (n = 82). The same samples were also tested with culture (ISO 6579: 2002) method, conventional and real-time PCR assays. Using the developed assay with 6-h enrichment step, it could give accurate results as that of the culture method. Conclusions: The results of this study showed that the SRCA assay is a rapid, simple, sophisticated equipment-free and user-friendly method for accurate detection of Salmonella in meat foods. To our information, this is the first study to deploy SRCA assay for screening foods for Salmonella. Significance and Impact of the Study: The developed SRCA assay is costeffective, easy-to-perform and equipment-free; therefore, it has the potential to replace other molecular detection methods for regular screening of Salmonella in foods in field laboratories.

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Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification

et al. 2021

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“…fection by expressing a number of toxic factors, cell wall-related adecines and secreted exoproteins [2]. Therefore, monitoring of S. aureus in a dairy environment with high sensitivity is very important for ensuring milk quality and food safety [3].…”

mentioning

confidence: 99%

Complete chromosome and plasmid sequences of Staphylococcus aureus strain JDFM SA01, isolated from a milk filter in Korean dairy farm

et al. 2020

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Staphylococcus aureus is a significant pathogen that can source a variety of illness worldwide. In this announcement, we report here the complete genome sequence of S. aureus strain JDFM SA01, isolated from a milk filter collected from Korean dairy farm. The final complete genome assembly consists of one circular chromosome (2,748,925 bp) with an overall GC content of 32.9% and one circular plasmid sequence (24,655 bp) with a GC content of 28.7%.

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Advancements in Molecular Techniques for the Detection of Foodborne Pathogens

Kumari

Sharma

Sanjukta

et al. 2022

Nanosensing and Bioanalytical Technologies in Food Quality Control

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Saltatory rolling circle amplification for sensitive visual detection of Staphylococcus aureus in milk

Cited by 33 publications

References 24 publications

Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification

Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification

Complete chromosome and plasmid sequences of Staphylococcus aureus strain JDFM SA01, isolated from a milk filter in Korean dairy farm

Advancements in Molecular Techniques for the Detection of Foodborne Pathogens

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