Salt Tolerance in Apple Seedlings is Affected by an Inhibitor of ABA 8′-Hydroxylase CYP707A

Sales, Lucas Santos; Ohara, Hitoshi; Ohkawa, Katsuya; Saito, Takanori; Todoroki, Yasushi; Srilaong, V.; Kondo, Satoru

doi:10.1007/s00344-017-9665-y

Cited by 12 publications

(7 citation statements)

References 26 publications

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“…Insertional mutants for the CYP707A1 [ 49 ] or CYP707A2 [ 21 ] genes display 6-fold higher ABA accumulation than the controls. Inhibitors of the enzyme 8′-hydroxylase have been used to increase the levels of ABA in apple [ 45 , 50 ] and to improve drought tolerance in Agrostis capillaris [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

et al. 2020

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Background Somatic embryogenesis in grapevines is a complex process that depends on many physiological and genetic factors. One of its main limitations is the process of precocious germination of the somatic embryos in differentiation medium. This process lowers plant conversion rates from the somatic embryos, and it is probably caused by a low endogenous abscisic acid (ABA) content. Results Precocious germination of the somatic embryos was successfully avoided by culturing grapevine cv. Mencía embryogenic aggregates over a semipermeable membrane extended on top of the differentiation medium. The weekly analysis of the endogenous ABA and ABA-glucosyl ester (ABA-GE) contents in the aggregates showed their rapid accumulation. The expression profiles of 9-cis-epoxycarotenoid dioxygenase (VvNCED1), 8′-hydroxylase (VvHyd2), UDP-glucosyltransferase (VvUGT) and β-glucosidase (VvBG2) genes in grapevine revealed that the occurrence of a first accumulation peak of endogenous ABA in the second week of culture over the semipermeable membrane was mainly dependent on the expression of the VvNCED1 gene. A second increase in the endogenous ABA content was observed in the fourth week of culture. At this point in the culture, our results suggest that of those genes involved in ABA accumulation, one (VvNCED1) was repressed, while another (VvBG2) was activated. Similarly, of those genes related to a reduction in ABA levels, one (VvUGT) was repressed while another (VvHyd2) was activated. The relative expression level of the VvNCED1 gene in embryogenic aggregates cultured under the same conditions and treated with exogenous ABA revealed the significant downregulation of this gene. Conclusions Our results demonstrated the involvement of ABA metabolism in the control of the maturation of grapevine somatic embryos cultured over a semipermeable membrane and two important control points for their endogenous ABA levels. Thus, subtle differences in the expression of the antagonistic genes that control ABA synthesis and degradation could be responsible for the final level of ABA during the maturation of grapevine somatic embryos in vitro. In addition, the treatment of somatic embryos with exogenous ABA suggested the feedback-based control of the expression of the VvNCED1 gene by ABA during the maturation of grapevine somatic embryos.

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Section: Resultsmentioning

confidence: 99%

Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

et al. 2020

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“…Over-expression of the OsNCED5 gene enhanced salt tolerance by controlling ABA biosynthesis and genes related to abiotic stress in rice (Oryza sativa L.) (Huang et al, 2019). Sales et al (2017) found that the inhibition of CYP707A1 attenuated the catabolism of ABA and increased endogenous ABA concentrations under NaCl stress and decreased Na + intake and mitigated salinity damage in apple seedlings. Generally, crosstalk exists between ABA and JA with regard to their signaling behavior (Yang et al, 2019).…”

Section: German Society For Horticultural Sciencementioning

confidence: 99%

“…(3) a group treated with 100 mM NaCl solution, without PDJ application (PDJ -NaCl + ); and (4) a group administered a combination of 100 mM NaCl and PDJ (PDJ + NaCl + ). The concentrations of PDJ and NaCl were based on a previous study by Sales et al (2017). A total of 240 seedlings were sampled from each group, divided into three replications, and healthy fresh leaves were collected at 1, 4, 7, and 10 days after the beginning of the NaCl treatment, frozen with liquid nitrogen, and kept at -80°C until further analysis.…”

Section: Plant Materials and Treatmentmentioning

confidence: 99%

“…The concentrations of H 2 O 2 in fresh leaves (0.5 g, three replications) were measured according to the method of nal concentration was calculated according to the standard curve derived from different concentrations of H 2 O 2 . The proline concentrations were assayed based on the method of Sales et al (2017). The fresh leaf tissues (0.5 g, three replications) were homogenized with 10 mL sulfosalicylic acid 3% (w/v) and then centrifuged at 15,000 g for 10 min.…”

Section: Measurement Of Hydrogen Peroxide (H 2 O 2 ) Proline Concentrations and Antioxidative Enzyme Activitymentioning

confidence: 99%

“…The chlorophyll a (Chl a), chlorophyll b (Chl b), and total chlorophyll concentrations were calculated according to Burton et al (1986). The Na + and K + concentrations in the leaves were determined according to Sales et al (2017). The leaf samples (0.5 g dry weight, three replications) were mixed with 15 mL nitric powder.…”

Section: Determination Of Chlorophyll Na + and K + Concentrationsmentioning

confidence: 99%

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Salt stress in apple seedlings was mitigated by n-propyl dihydrojasmonate, a synthetic analog of jasmonic acid

Wang¹,

Wang²,

Tomiyama³

et al. 2021

Europ.J.Hortic.Sci.

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The effects of n-propyl dihydrojasmonate (PDJ) application, which is a synthetic analog of jasmonic acid (JA) against sodium chloride (NaCl) stress in apple seedlings, were examined. The malondialdehyde (MDA) and proline concentrations, endogenous JA and abscisic acid (ABA) concentrations, antioxidant activities, Na + and K + concentrations, along with 9-cis-epoxycarotenoid deoxygenase 1 (MdNCED1), ABA 8'-hydroxylase 1 (MdCYP707A1), allene oxide synthase 1 (MdAOS1), jasmonate resistant (MdJAR), jasmonate ZIM-domain 2 (MdJAZ2), synthetase ( ), ornithine aminotransferase 1 (MdOAT1), proline dehydrogenase (MdPDH), salt overly sensitive 1 (MdSOS1) and Na + /H + exchanger 1 (MdNHX1) gene expressions in the leaves were analyzed. NaCl treatment without PDJ application (PDJ -NaCl + tions and superoxide (SOD), peroxidase (POD) and catalase (CAT) activities, but increased the ion leakage, MDA, proline, and Na + concentrations, as well as the hydrogen peroxide (H 2 O 2 ) and endogenous JA and ABA concentrations in the leaves. Foliar application of PDJ before NaCl treatment (PDJ + NaCl + ) decreased Na + concentrations, ion leakage, MDA, proline accumulation and H 2 O 2 generation, but increased K + concentrations, antioxidant capacity, and chlorophyll concentrations in the leaves. In the PDJ + NaClgroup, although the JA and ABA concentrations and MdAOS1, MdJAR, and MdNCED1 gene expressions were increased, the MdJAZ2 and MdCYP707A1 gene expressions were decreased. In addition, the PDJ + NaCl + group showed higher MdPDH, MdSOS1, and MdNHX1 gene expressions and lower gene expressions than the PDJ -NaCl + group at 7 and 10 days after treatment. The present data suggested that PDJ application could induce rapidly endogenous JA and ABA, enhance SOD, POD, and CAT activities, decrease the Na + uptake, and mitigate NaCl stress.

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Inhibition of Abscisic Acid 8′-Hydroxylase Affects Dehydration Tolerance and Root Formation in Cuttings of Grapes (Vitis labrusca L. × Vitis vinifera L. cv. Kyoho) Under Drought Stress Conditions

Tomiyama

Sato

Opio

et al. 2020

J Plant Growth Regul

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Salt Tolerance in Apple Seedlings is Affected by an Inhibitor of ABA 8′-Hydroxylase CYP707A

Cited by 12 publications

References 26 publications

Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

Changes in abscisic acid metabolism in relation to the maturation of grapevine (Vitis vinifera L., cv. Mencía) somatic embryos

Salt stress in apple seedlings was mitigated by n-propyl dihydrojasmonate, a synthetic analog of jasmonic acid

Inhibition of Abscisic Acid 8′-Hydroxylase Affects Dehydration Tolerance and Root Formation in Cuttings of Grapes (Vitis labrusca L. × Vitis vinifera L. cv. Kyoho) Under Drought Stress Conditions

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