Salt stress induces an increased expression of V-type H+-ATPase in mature sugar beet leaves

Kirsch, Matthias; Zhigang, An; Viereck, Ruth; Löw, Rainer; Rausch, Thomas

doi:10.1007/bf00019107

Cited by 67 publications

(39 citation statements)

References 25 publications

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“…Thus, it would appear that they are able to compartmentalize Na + in vacuoles in order to adapt to osmotic stress and avoid toxicity. In fact, in spinach a strict compartmentalisation of Na + and Clin the vacuole has been demonstrated (Coughlan and Wyn Jones 1980), and in sugar beet leaves saline stress induced a marked activation of the V-ATPase, increasing the proton-motive force that is used by Na + /H + antiports through the tonoplast (Kirsch et al 1996). Our results with beetroot coincide with those from sugar beet, which indicate that established plants showed high osmotic adjustment and accumulation of gycinebetaine, proline and inorganic ions (Na + , K + and Cl -) under saline stress (Heuer and Plaut 1989;Gzik 1996;Ghoulam et al 2002).…”

Section: Discussionmentioning

confidence: 98%

Changes in free polyamine concentration induced by salt stress in seedlings of different species

et al. 2008

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Growth rate, mineral composition and changes in polyamine concentration induced in response to salinity were studied in six crop species: spinach, lettuce, bean, pepper, beetroot and tomato. Salinity decreased growth rate, but sensitivity differed amongst the species: pepper being the most sensitive, followed by bean, tomato, lettuce and spinach, with beetroot being the most tolerant. The increase of Na + and total cation with salinity in shoots was the highest in spinach and beetroot, the most tolerant species, while in pepper it was the lowest. Changes in putrescine (Put) concentration in shoots were related to salinity tolerance (increased in the most sensitive), while changes in spermidine (Spd; decreases) and spermine (Spm; increases) were similar with most species, except for pepper in which salinity strongly increased Put, Spd and Spm. Therefore, total polyamine concentration increased in pepper shoot, while it decreased in the other species. Thus, results show that Put accumulation was a consequence of salt stress in the most sensitive species, while salt tolerant species (beetroot) showed little change in polyamine concentration, and higher concentration in both Na + and total cations. The role of polyamines or cation increased concentration after saline treatment in species with different salt tolerance is discussed.

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Section: Discussionmentioning

confidence: 98%

Changes in free polyamine concentration induced by salt stress in seedlings of different species

et al. 2008

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“…Indeed, previous work suggested that vacuolar sodium accumulation in salt-tolerant plants may be mediated by a tonoplast Na ϩ ͞H ϩ antiporter that utilizes the protonmotive force generated by the vacuolar H ϩ -ATPase (VATPase) and͞or H ϩ -translocating pyrophosphatase (VPPase; refs. [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast

Gaxiola

Rao

Sherman

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

543

375

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Overexpression of the Arabidopsis thaliana vacuolar H ؉ -pyrophosphatase (AVP1) confers salt tolerance to the salt-sensitive ena1 mutant of Saccharomyces cerevisiae. Suppression of salt sensitivity requires two ion transporters, the Gef1 Cl ؊ channel and the Nhx1 Na ؉ ͞H ؉ exchanger. These two proteins colocalize to the prevacuolar compartment of yeast and are thought to be required for optimal acidification of this compartment. Overexpression of AtNHX1, the plant homologue of the yeast Na ؉ ͞H ؉ exchanger, suppresses some of the mutant phenotypes of the yeast nhx1 mutant. Moreover, the level of AtNHX1 mRNA in Arabidopsis is increased in the presence of NaCl. The regulation of AtNHX1 by NaCl and the ability of the plant gene to suppress the yeast nhx1 mutant suggest that the mechanism by which cations are detoxified in yeast and plants may be similar.

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“…Similarly, it has been shown that NaCl but not ABA can stimulate V-ATPase activity (20). Salt-induced transcript up-regulation of one or more V-ATPase subunits or an increase in V-ATPase activity has also been observed in a number of glycophytic species (3,4,22,30,45,61). This includes A. thaliana, where an increase in the transcript levels of AtVHA-C (36) but not of subunit D (AtVHA-D) (23) was found upon salt stress.…”

mentioning

confidence: 93%

SOS2 Promotes Salt Tolerance in Part by Interacting with the Vacuolar H⁺-ATPase and Upregulating Its Transport Activity

Batelli

Verslues

Agius

et al. 2007

Molecular and Cellular Biology

231

171

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The salt overly sensitive (SOS) pathway is critical for plant salt stress tolerance and has a key role in regulating ion transport under salt stress. To further investigate salt tolerance factors regulated by the SOS pathway, we expressed an N-terminal fusion of the improved tandem affinity purification tag to SOS2 (NTAP-SOS2) in sos2-2 mutant plants. Expression of NTAP-SOS2 rescued the salt tolerance defect of sos2-2 plants, indicating that the fusion protein was functional in vivo. Tandem affinity purification of NTAP-SOS2-containing protein complexes and subsequent liquid chromatography-tandem mass spectrometry analysis indicated that subunits A, B, C, E, and G of the peripheral cytoplasmic domain of the vacuolar H ؉ -ATPase (V-ATPase) were present in a SOS2-containing protein complex. Parallel purification of samples from control and saltstressed NTAP-SOS2/sos2-2 plants demonstrated that each of these V-ATPase subunits was more abundant in NTAP-SOS2 complexes isolated from salt-stressed plants, suggesting that the interaction may be enhanced by salt stress. Yeast two-hybrid analysis showed that SOS2 interacted directly with V-ATPase regulatory subunits B1 and B2. The importance of the SOS2 interaction with the V-ATPase was shown at the cellular level by reduced H ؉ transport activity of tonoplast vesicles isolated from sos2-2 cells relative to vesicles from wild-type cells. In addition, seedlings of the det3 mutant, which has reduced V-ATPase activity, were found to be severely salt sensitive. Our results suggest that regulation of V-ATPase activity is an additional key function of SOS2 in coordinating changes in ion transport during salt stress and in promoting salt tolerance.To cope with salt stress, plants have evolved strategies to maintain low Na ϩ concentrations in the cytoplasm. The salt overly sensitive (SOS) pathway, identified through isolation and study of the sos1, sos2, and sos3 mutants, is essential for maintaining favorable ion ratios in the cytoplasm and for tolerance of salt stress (63, 64). SOS1 is a Na ϩ /H ϩ exchanger located on the plasma membrane (39, 53); SOS3 is a myristoylated EF hand-type Ca 2ϩ -binding protein able to sense specific salt stress-induced calcium signals (19), and SOS2 is a Ser/Thr kinase with a C-terminal regulatory domain and an N-terminal catalytic domain (24). During salt stress conditions, the SOS2-SOS3 complex phosphorylates and activates the transport activity of the SOS1 antiporter (42).The function of the SOS2-SOS3 regulatory complex depends on interaction of SOS2 and regulatory proteins, including SOS3. The C-terminal regulatory domain of SOS2 consists of an autoinhibitory FISL motif that binds to SOS3 (13, 24) and a PPI motif that binds to type 2C protein phosphatase abcisic acid (ABA)-insensitive 2 (ABI2) (33). Yeast two-hybrid experiments have shown that the SOS2 protein physically interacts with SOS3, and in vitro phosphorylation assays have shown that Ca 2ϩ is required to activate the kinase activity of the SOS2-SOS3 complex (16). SOS3 binding also recr...

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Salt stress induces an increased expression of V-type H+-ATPase in mature sugar beet leaves

Cited by 67 publications

References 25 publications

Changes in free polyamine concentration induced by salt stress in seedlings of different species

Changes in free polyamine concentration induced by salt stress in seedlings of different species

The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast

SOS2 Promotes Salt Tolerance in Part by Interacting with the Vacuolar H⁺-ATPase and Upregulating Its Transport Activity

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Salt stress induces an increased expression of V-type H+-ATPase in mature sugar beet leaves

Cited by 67 publications

References 25 publications

Changes in free polyamine concentration induced by salt stress in seedlings of different species

Changes in free polyamine concentration induced by salt stress in seedlings of different species

The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast

SOS2 Promotes Salt Tolerance in Part by Interacting with the Vacuolar H+-ATPase and Upregulating Its Transport Activity

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SOS2 Promotes Salt Tolerance in Part by Interacting with the Vacuolar H⁺-ATPase and Upregulating Its Transport Activity