Salt stress induced proteome and transcriptome changes in sugar beet monosomic addition line M14

Yang, Le; Ma, Castello; Wang, Linlin; Chen, Sixue; Li, Haiying

doi:10.1016/j.jplph.2012.01.023

Cited by 91 publications

(83 citation statements)

References 55 publications

(51 reference statements)

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“…However, in roots of 300 mM NaHCO 3 -stressed T. hispida , most genes encoding ribosomal proteins were down-regulated at 12 h, but up-regulated at 48 h13. Ribosomal proteins were increased in roots of sugar beet71 and cucumber72 after 7 days of 50 mM and 500 mM NaCl stress. These results indicate that protein synthesis machinery in roots tends to be inhibited by short-term alkali/salt stress, but it can be activated after a long-term period of stress.…”

Section: Discussionmentioning

confidence: 94%

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Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses

Zhao

Suo

Chen

et al. 2016

Sci Rep

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Soil alkalization severely affects crop growth and agricultural productivity. Alkali salts impose ionic, osmotic, and high pH stresses on plants. The alkali tolerance molecular mechanism in roots from halophyte Puccinellia tenuiflora is still unclear. Here, the changes associated with Na2CO3 tolerance in P. tenuiflora roots were assessed using physiological and iTRAQ-based quantitative proteomic analyses. We set up the first protein dataset in P. tenuiflora roots containing 2,671 non-redundant proteins. Our results showed that Na2CO3 slightly inhibited root growth, caused ROS accumulation, cell membrane damage, and ion imbalance, as well as reduction of transport and protein synthesis/turnover. The Na2CO3-responsive patterns of 72 proteins highlighted specific signaling and metabolic pathways in roots. Ca2+ signaling was activated to transmit alkali stress signals as inferred by the accumulation of calcium-binding proteins. Additionally, the activities of peroxidase and glutathione peroxidase, and the peroxiredoxin abundance were increased for ROS scavenging. Furthermore, ion toxicity was relieved through Na+ influx restriction and compartmentalization, and osmotic homeostasis reestablishment due to glycine betaine accumulation. Importantly, two transcription factors were increased for regulating specific alkali-responsive gene expression. Carbohydrate metabolism-related enzymes were increased for providing energy and carbon skeletons for cellular metabolism. All these provide new insights into alkali-tolerant mechanisms in roots.

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Section: Discussionmentioning

confidence: 94%

“…In addition, homologous CPN60 and Hsp90 were down-regulated in P. tenuiflora seedlings under 150 mM and 200 mM Na 2 CO 3 for 12 h17 (Fig. 7), and Hsp90 was decreased in NaCl-stressed roots of sugar beet71 and creeping bentgrass ( Agrostis stolonifera )74. NAC acts as a component of ribosome-associated chaperones.…”

Section: Discussionmentioning

confidence: 97%

Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses

Zhao

Suo

Chen

et al. 2016

Sci Rep

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“…More than 6% of land throughout the globe is affected by salinization [4]. Thus, improving the salt tolerance of crops to utilize saline soil is of high urgency [5,6]. High concentrations of salt usually lead to ionic imbalance, oxidative damage and nutrient deficiency in plants [2].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress

Wang

et al. 2017

IJMS

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The sugar beet monosomic addition line M14 is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss, and shows tolerance to salt stress. Our study focuses on exploring the molecular mechanism of the salt tolerance of the sugar beet M14. In order to identify differentially expressed genes in M14 under salt stress, a subtractive cDNA library was generated by suppression subtractive hybridization (SSH). A total of 36 unique sequences were identified in the library and their putative functions were analyzed. One of the genes, S-adenosylmethionine synthetase (SAMS), is the key enzyme involved in the biosynthesis of S-adenosylmethionine (SAM), a precursor of polyamines. To determine the potential role of SAMS in salt tolerance, we isolated BvM14-SAMS2 from the salt-tolerant sugar beet M14. The expression of BvM14-SAMS2 in leaves and roots was greatly induced by salt stress. Overexpression of BvM14-SAMS2 in Arabidopsis resulted in enhanced salt and H2O2 tolerance. Furthermore, we obtained a knock-down T-DNA insertion mutant of AtSAMS3, which shares the highest homology with BvM14-SAMS2. Interestingly, the mutant atsam3 showed sensitivity to salt and H2O2 stress. We also found that the antioxidant system and polyamine metabolism play an important role in salt and H2O2 tolerance in the BvM14-SAMS2-overexpressed plants. To our knowledge, the function of the sugar beet SAMS has not been reported before. Our results have provided new insights into SAMS functions in sugar beet.

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“…Bu durum, V-ATPaz'ın iyon zararından hücreleri korumada kritik bir öneme sahip olduğunu göstermektedir. V-ATPaz'ın sentezindeki artış, tuz stresine girmiş bazı bitkilerde bulunmuştur [9,38,43]. Vakuolar H + -ATPaz'ların artan seviyeleri ve/veya aktiviteleri, tuz stresi altında Na + ayrıştırması ve ozmotik ayarlama için oldukça etkili strateji olarak değerlendirilmiştir [25].…”

Section: İyon Homeostazisiunclassified

“…Bazı kütle spektrometresi cihazları (MALDI-TOF/TOF MS ve LC-MS/MS gibi) protein kimliklendirmesi için kullanılmaktadır. Bu teknolojiler temelinde, mevcut proteomik çalışmalar ile bazı proteinlerin tuza tepki olarak sentezlendiği belirlenmiştir [8][9][10]. Ozmotin [11], reaktif oksijen türleri ile savaşan enzimler [12] ve patojen ilişkili [13] proteinler gibi stres proteinleri tuza tolerans geliştirmede önemli moleküler markörler olarak kullanılmıştır.…”

Section: Introductionunclassified

Tuz Stresi Altındaki Bitkilerin Metabolik Yollarındaki Proteom Değişimleri

Yıldız¹,

Terzi²,

Akçalı³

2014

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi

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ÖzetBitkilerde tuz stresine tolerans transkriptom, proteom ve metabolom kompozisyonundaki değişikliklere eşlik eden gen ekspresyonundaki önemli değişiklikleri kapsamaktadır. Proteinler direkt olarak stres cevaplarında fonksiyon görmektedir. Bu nedenle, proteomik çalışmalar tuz stresine tolerans ve fonksiyonel proteinler arasındaki muhtemel ilişkileri açıklayabilmektedir. Tuz stresine cevapta fonksiyon gören proteinlerin teşhisi tuza toleranslı transgenik bitkilerin geliştirilmesinde önemlidir. Bu derlemede, bitkilerin tuz stresine cevaplarını analiz etmek için yapılan son proteomik çalışmalar, tuz stresine cevapta muhtemel mekanizmalar ve metabolik yollarda fonksiyon gören proteinlerin seviyesindeki değişimler tartışılmıştır.Anahtar Kelimeler: Bitki, Tuz toleransı, Proteomik. Proteom Changes in Metabolic Pathways of Plants under Salt Stress AbstractTolerance against salt stress in plants includes substantial changes in gene expression which is accompanied with changes in composition of transcriptome, metabolome and proteome. Proteins are directly involved in plant stress response. Therefore, proteomics studies can explain the possible relationships between salinity tolerance and functional proteins. The identification of the proteins that are involved in responses to salinity stress could lead to the development of transgenic plants that have an enhanced tolerance to salinity. In this review, the recent proteomics studies to the analysis of the responses of plants to salinity stress, the possible mechanisms in response to salinity stress and the changes in the level of proteins involved in metabolic pathways are discussed.

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Salt stress induced proteome and transcriptome changes in sugar beet monosomic addition line M14

Cited by 91 publications

References 55 publications

Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses

Na2CO3-responsive mechanisms in halophyte Puccinellia tenuiflora roots revealed by physiological and proteomic analyses

Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress

Tuz Stresi Altındaki Bitkilerin Metabolik Yollarındaki Proteom Değişimleri

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