Salt Stress Induced Nuclear and DNA Degradation in Meristematic Cells of Barley Roots

Katsuhara, Maki; Kawasaki, Toshio

doi:10.1093/oxfordjournals.pcp.a028928

Cited by 159 publications

(107 citation statements)

References 11 publications

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“…The inhibitory effects of salt stress on mitotic activity have been known for a long time (Katsuhara and Kawasaki 1996, Lutsenko et al 2005, Radic et al 2005, Tabur and Demir 2009, 2010a. On the other hand, the detrimental effects of salt stress on chromosomal abnormalities have recently begun to be studied (Radic et al 2005, Tabur and Demir 2009, 2010a.…”

Section: Physiological and Cytogenetical Effects Of Exogenous Avle Inmentioning

confidence: 99%

The Effects of Aloe vera L. Leaf Extract on Some Physiological and Cytogenetical Parameters in Allium cepa L. Seeds Germinated under Salt Stress

2016

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SummaryThe present study was performed to evaluate the role of Aloe vera L. leaf extract (AvLE) on some physiological and cytogenetical parameters of Allium cepa L. seeds exposed to salinity. The radicle length of the seeds germinated in the medium with AvLE alone increased as compared with ones of the control seeds germinated in distilled water medium, while their radicle number and fresh weight reduced according to the control. In addition, the germination percentage of the mentioned seeds statistically was the same as the control seeds. Furthermore, the seeds germinated in the media containing 0.1 mg/L AvLE alone showed a significant increase of mitotic index, although they exhibited a higher number of chromosomal aberrations and micronucleus (MN) formation as compared with the seeds germinated in control conditions. On the other hand, salt stress considerably inhibited the seed germination and seedling growth of A. cepa. Moreover, salinity markedly decreased the mitotic index in root tip meristems of the seeds and increased the number of chromosomal aberrations. The negative effect of salt on the seed germination, seedling growth, mitotic activity and MN formation was alleviated in dramatically varying degrees by AvLE application. In contrast, the detrimental effects of salt on the chromosomal aberrations greatly increased with this treatment. Consequently, we reported that the application of AvLE alone induced chromosomal aberrations and MN formation, which are an indicator of genotoxicity, and had cytotoxic activity in normal conditions. However, the frequency of MN is greatly reduced in root tip cells of the seeds germinated in the medium containing 0.1 mg/L AvLE +0.15 M NaCl.

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Section: Physiological and Cytogenetical Effects Of Exogenous Avle Inmentioning

confidence: 99%

The Effects of Aloe vera L. Leaf Extract on Some Physiological and Cytogenetical Parameters in Allium cepa L. Seeds Germinated under Salt Stress

2016

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“…It was shown that salt (NaCl) stress induces apoptosis-like cell death in barley and Arabidopsis roots (47)(48)(49). In Arabidopsis, PCD phenotypes (TUNEL-positive nuclei and DNA laddering) induced by a sublethal dose of salt treatment (0.16 M NaCl) were observed in the root meristematic and elongation/differentiation zones, whereas root cells of salt-hypersensitive mutant plants (sos1) exhibited more severe PCD phenotype under salt stress (49).…”

Section: Role Of Atbi1 In Er Stress and Its Related Cell Death Process-mentioning

confidence: 99%

BAX Inhibitor-1 Modulates Endoplasmic Reticulum Stress-mediated Programmed Cell Death in Arabidopsis

Watanabe

Lam

2008

Journal of Biological Chemistry

188

224

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The components and pathways that regulate programmed cell death (PCD) in plants remain poorly understood. Here we describe the impact of drug-induced endoplasmic reticulum (ER) stress on Arabidopsis seedlings and present evidence for the role of Arabidopsis BAX inhibitor-1 (AtBI1) as a modulator of ER stress-mediated PCD. We found that treatment of Arabidopsis seedlings with tunicamycin (TM), an inhibitor of N-linked glycosylation and an inducer of ER stress by triggering accumulation of unfolded proteins in the ER, results in strong inhibition of root growth and loss of survival accompanied by typical hallmarks of PCD such as accumulation of H 2 O 2 , chromatin condensation, and oligonucleosomal fragmentation of nuclear DNA. These phenotypes are alleviated by co-treatment with either of two different chemical chaperones, sodium 4-phenylbutyrate and tauroursodeoxycholic acid, both with chaperone properties that can reduce the load of misfolded protein in the ER. Expression of AtBI1 mRNA and its promoter activity are increased dramatically prior to initiation of TM-induced PCD. Compared with wild-type plants, two AtBI1 mutants (atbi1-1 and atbi1-2) exhibit hypersensitivity to TM with accelerated PCD progression. Conversely, overexpressing AtBI1 markedly reduces the sensitivity of Arabidopsis seedlings to TM. However, alterations in AtBI1 gene expression levels do not cause a significant effect on the expression patterns of typical ER stressinducible genes (AtBip2, AtPDI, AtCRT1, and AtCNX1). We propose that AtBI1 plays a pivotal role as a highly conserved survival factor during ER stress that acts in parallel to the unfolded protein response pathway.

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“…So, it is essential for plant development, survival and maintaining tissue and organ homeostasis (Greenberg, 1996;Van Breusegem and Dat, 2006). In addition, many stressful environments such as drought, salt, heat shock, toxic chemicals and pathogens can also induce cell death in plants (Katsuhara and Kawasaki, 1996;Dannon and Gallois, 1998;Munné-Bosch and Alegre, 2004;Overmyer et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Hypoxia-Induced Programmed Cell Death in Root-Tip Meristematic Cells of Triticum aestivum L.

Pang¹,

Zhang²

2015

Acta Biologica Cracoviensia S. Botanica

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In this study, wheat (Triticum aestivum L.) roots were treated with hypoxic water. The staining of cell preparations with DAPI revealed morphological changes of the cells such as nuclear condensation, deformation and fragmentation. Under TEM, cellular membrane shrinkage and breakage, chromatin condensation and apoptotic-like bodies were displayed. The number of mitochondria increased dramatically; their cristae were damaged; the interior became a cavitation and only some flocculent materials were distributed. Indirect immunofluorescence staining indicated that cytochrome C diffused from mitochondria to nucleoplasm and cytoplasm. TUNEL positive nuclei indicated double strand breaks of DNA. DAB staining was used for the identification of hydrogen peroxide and examination showed that the longer the treating time, the darker the staining of the meristematic zones of the roots which suggested the increased accumulation of these Reactive Oxygen Species (ROS). The elevation of hydrogen peroxide production was paralleled with the increase of SOD and POD activities. A negative correlation between the exposure time under hypoxia and the contents of soluble proteins was found. No obvious effect of hypoxia on MDA was established. The obtained results demonstrate that hypoxia causes programmed cell death in the root-tip meristematic cells of Triticum aestivum L. which is most probably attributed to the accumulation of large amounts of ROS.K Ke ey y w wo or rd ds s: : Hypoxic water, reactive of oxygen species, programmed cell death, root-tip meristematic cell, Triticum aestivum L.

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Salt Stress Induced Nuclear and DNA Degradation in Meristematic Cells of Barley Roots

Cited by 159 publications

References 11 publications

The Effects of <i>Aloe vera</i> L. Leaf Extract on Some Physiological and Cytogenetical Parameters in <i>Allium cepa</i> L. Seeds Germinated under Salt Stress

The Effects of <i>Aloe vera</i> L. Leaf Extract on Some Physiological and Cytogenetical Parameters in <i>Allium cepa</i> L. Seeds Germinated under Salt Stress

BAX Inhibitor-1 Modulates Endoplasmic Reticulum Stress-mediated Programmed Cell Death in Arabidopsis

Hypoxia-Induced Programmed Cell Death in Root-Tip Meristematic Cells of Triticum aestivum L.

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