Salt stress-induced modulations in the shoot proteome of Brassica juncea genotypes

Abstract

Indian mustard [Brassica juncea (L.) Czern and Coss] is cultivated mainly in the northwestern agroclimatic region of India and suffers huge losses in productivity due to salinization. In an effort to figure out adaptation strategies of Indian mustard to salt stress, we conducted a comparative proteome analysis of shoots of its two genotypes, with contrasting sensitivity to salt stress. Differential expression of 21 proteins was observed during the two-dimensional electrophoresis (2DE). The identified salt-stress-responsive proteins were associated with different functional processes including osmoregulation, photosynthesis, carbohydrate metabolism, ion homeostasis, protein synthesis and stabilization, energy metabolism, and antioxidant defense system. Salt-tolerant genotype (CS-52) showed a relatively higher expression of proteins involved in turgor regulation, stabilization of photosystems and proteins, and salt compartmentalization, as compared to salt-sensitive genotype (Pusa Varuna). Our results suggest that modulating the expression of salt-responsive proteins can pave the way for developing salt tolerance in the Indian mustard plants.

Salicylic acid alleviates salinity-caused damage to foliar functions, plant growth and antioxidant system in Ethiopian mustard (Brassica carinata A. Br.)

“…The present study indicates that the salinity-induced loss of the growth and biomass of B. carinata is relatively stronger in cultivar Merawi than in Adet. The saline environment in the soil influences water imbibition by roots due to low osmotic potential of the substrate, besides hampering the phenomena of photosynthesis, protein synthesis, nutrient homeostasis, compatible solutes accumulation and the antioxidant defense mechanisms [5,8,25,53]. The salinity-caused decline in growth and biomass of B. carinata cultivars might be due to reduced leaf area, imbalance in plant water status and low production of photoassimilates [3][4][5]54].…”

Zinc application mitigates the adverse effects of NaCl stress on mustard [Brassica juncea (L.) Czern & Coss] through modulating compatible organic solutes, antioxidant enzymes, and flavonoid content

“…Zinc application considerably reduces the salinity-caused damage because of its positive effects on the uptake and partitioning of important mineral elements (Weisany et al 2011(Weisany et al , 2014. It improves plant growth by increasing the natural auxin (indole acetic acid) production and, consequently, activating the cell division and enlargement (Ali and Mahmoud 2013), maintaining membrane structural integrity (Weisany et al 2014), accumulating phospholipids (Jiang et al 2014), improving protein synthesis (Ebrahimian and Bybordi 2011), scavenging free oxygen radicals (Jiang et al 2014), mediating nutrient translocation from developing cells (Jiang et al 2014), and restricting excessive Na + and Cl − uptake (Weisany et al 2011;Siddiqui et al 2015;Yousuf et al 2016aYousuf et al , 2016b.…”

The impact of cerium oxide nanoparticles on the salt stress responses of Brassica napus L.

“…All plants were fert-irrigated with full strength Hoagland (Hoagland and Arnon, 1950) for 40 days. The NaCl concentration was chosen because 60-120 mM (Yousuf et al, 2016;Zaghdoud et al, 2016) was deemed as the average salinity in saline soil and brackish water in most previous studies. Starting from day 12 (one week after NaCl treatment), the chlorophyll fluorescence, net photosynthesis rate and gas exchange rate were measured weekly until the end of experiment.…”