Salt stress-induced invasiveness of major <i>Listeria monocytogenes</i>
 serotypes

Wałecka-Zacharska, Ewa; Kosek-Paszkowska, Katarzyna; Bania, Jacek; Karpíšková, Renata; Stefaniak, Tadeusz

doi:10.1111/lam.12036

Cited by 20 publications

(12 citation statements)

References 24 publications

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“…Both lineage I and lineage II comprised invasive and weakly invasive strains, which varied in the ability to invade HT-29 cells up to 7.6 and almost 3 orders of magnitude, respectively. The most invasive were serogroup 4b(d,e) strains, which agrees with the results of our previous study (Wałecka-Zacharska et al 2013).…”

Section: Discussionsupporting

confidence: 83%

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Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

Wałecka-Zacharska¹,

Kosek-Paszkowska²,

Bania³

et al. 2015

Polish Journal of Veterinary Sciences

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Animals are important reservoir of Listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness of L. monocytogenes strains of animal origin is very scarce. Ability of 18 L. monocytogenes strains of animal origin to invade HT-29 cells was investigated. Plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. Almost 40% of L. monocytogenes strains were weakly invasive. It was shown that strains from serogroup 4b exhibited the highest invasiveness, whereas serogroup 1/2b consisted of strains of invasiveness below 0.0001%. Analysis of translated inlA and inlB gene sequences revealed no premature stop codons. Lineage-specific mutations in low invasive strains were identified within inlA and inlB sequences. Our results demonstrate high incidence of low invasive animal L. monocytogenes strains, which may be at least partly explained by unique point mutations in the InlA and InlB.

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Section: Discussionsupporting

confidence: 83%

“…Comparison of results presented here with our previous data on L. monocytogenes strains from human clinical cases (n=14) and food (n= 27) demonstrates that low invasive strains are more prevalent among animal population than human and food isolates (Wałecka-Zacharska et al 2013).…”

Section: Discussionmentioning

confidence: 67%

Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

Wałecka-Zacharska¹,

Kosek-Paszkowska²,

Bania³

et al. 2015

Polish Journal of Veterinary Sciences

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“…The human small intestine has an osmolarity equivalent to 0.3 M NaCl, requiring gastrointestinal pathogens to be highly tolerant to salt stress in order to persist in this environment and to further invade other tissues (Sleator et al ., ). Exposure to elevated osmolarity induces the expression of the σ B regulon for general stress responses and GI epithelial cell invasion (Abram et al ., ; Bae et al ., ; Walecka‐Zacharska et al ., ). Furthermore, σ B also controls the transcription of PrfA, the master virulence regulator, and the σ B and PrfA regulons significantly overlap, further emphasizing the role of osmotic stress as a primary stress and virulence signal (Ollinger et al ., ; Chaturongakul et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Cyclic di‐AMP targets the cystathionine beta‐synthase domain of the osmolyte transporter OpuC

Huynh

Choi

Sureka

et al. 2016

Molecular Microbiology

111

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SUMMARY Cellular turgor is of fundamental importance to bacterial growth and survival. Changes in external osmolarity as a consequence of fluctuating environmental conditions and colonization of diverse environments can significantly impact cytoplasmic water content, resulting in cellular lysis or plasmolysis. To ensure maintenance of appropriate cellular turgor, bacteria import ions and small organic osmolytes, deemed compatible solutes, to equilibrate cytoplasmic osmolarity with the extracellular environment. Here, we show that elevated levels of c-di-AMP, a ubiquitous second messenger among bacteria, result in significant susceptibility to elevated osmotic stress in the bacterial pathogen Listeria monocytogenes. We found that levels of import of the compatible solute carnitine show an inverse correlation with intracellular c-di-AMP content and that c-di-AMP directly binds to the CBS domain of the ATPase subunit of the carnitine importer OpuC. Biochemical and structural studies identify conserved residues required for this interaction and transport activity in bacterial cells. Overall, these studies reveal a role for c-di-AMP mediated regulation of compatible solute import and provide new insight into the molecular mechanisms by which this essential second messenger impacts bacterial physiology and adaptation to changing environmental conditions.

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“…In L. monocytogenes, expression of the a B factor is strongly induced by salt stress (3). Walecka-Zacharska et al (36) concluded that L. monocytogenes enhanced invasiveness in response to salt stress. Overexpression of the gene regulators ctsR, bvrA, and bvrC was observed, especially after the treatment of acid-and salt-stressed cells with synthetic pancreatic fluid.…”

Section: Discussionmentioning

confidence: 99%

“…Low pH and a high salt content may cause changes in gene expression as a result of the adaptation of L. monocytogenes to these stresses (1,13,24,36). pH values of 5.5 and a salt content of 4.5% are often found in fermented food, such as in fermented sausages, and these conditions (acid and salt stress) have therefore been used in the current microarray experiment to investigate the gene regulation of L. monocytogenes.…”

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confidence: 99%

Gene Transcription Patterns of pH- and Salt-Stressed Listeria monocytogenes Cells in Simulated Gastric and Pancreatic Conditions

Mataragas

Greppi

Rantsiou

et al. 2014

Journal of Food Protection

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A Listeria monocytogenes subgenomic array, targeting 54 genes involved in the adhesion, adaptation, intracellular life cycle, invasion, and regulation of the infection cycle was used to investigate the gene expression patterns of acid- and salt-stressed Listeria cells after exposure to conditions similar to those in gastric and pancreatic fluids. Three L. monocytogenes strains, one laboratory reference strain (EGDe) and two food isolates (wild strain 12 isolated from milk and wild strain 3 isolated from fermented sausage), were used during the studies. Differences in the expressed genes were observed between the gastric and pancreatic treatments and also between the serotypes. Increased transcripts were observed of the genes belonging to the adaptation and regulation group for serotype 4b (strain 12) and to the invasion and regulation group for serotype 1/2a (strain EGDe). Interestingly, no significantly differentially expressed genes were found for serotype 3c (strain 3) in most cases. The genes related to adaptation (serotype 1/2a) and to intracellular life cycle and invasion (serotype 4b) were down-regulated in order to cope with the hostile environment of the gastric and pancreatic fluids. These findings may provide experimental evidence for the dominance of serotypes 1/2a and 4b in clinical cases of listeriosis and for the sporadic occurrence of serotype 3c.

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Salt stress-induced invasiveness of major Listeria monocytogenes serotypes

Cited by 20 publications

References 24 publications

Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

Invasiveness of Listeria monocytogenes strains isolated from animals in Poland

Cyclic di‐AMP targets the cystathionine beta‐synthase domain of the osmolyte transporter OpuC

Gene Transcription Patterns of pH- and Salt-Stressed Listeria monocytogenes Cells in Simulated Gastric and Pancreatic Conditions

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