Salt-stress induced alterations in the root lipidome of two barley genotypes with contrasting responses to salinity

Abstract

Changes in lipid metabolism and composition as well as in distinct lipid species have been linked with altered plant growth, development and responses to environmental stresses including salinity. However, there is little information available in the literature focusing on lipids in roots under soil-related stresses such as salinity. Barley (Hordeum vulgare L.) is a major cereal grain and, as a glycophyte, suffers substantial yield loss when grown under saline conditions. Relatively little is understood of adaptation and tolerance mechanisms involving lipids and lipid metabolism in barley roots during development and under exposure to salinity stress. In this study we investigated the lipid composition of barley roots of Clipper and Sahara – two genotypes with contrasting responses to salinity – before and after salinity stress using a combination of three lipidomics techniques: Fatty acid compositional analysis, untargeted lipid profiling, and targeted analysis to profile quantitatively the individual molecular species of key plant lipid classes. Our results provide new insight into the effect of salinity on fatty acid profiles and key lipid classes within barley roots of two different genotypes, which is discussed in the context of current knowledge of the root metabolic responses of cereal crops to salinity stress.

Up‐regulation of lipid metabolism and glycine betaine synthesis are associated with choline‐induced salt tolerance in halophytic seashore paspalum

“…Lipid metabolism plays important roles in plant tolerance to salt stress (Chalbi et al, 2015;Natera et al, 2016). However, lipid synthesis is inhibited by salt stress, as demonstrated by a reduction in total lipid content and phospholipid content in various plant species, including wheat (Triticum aestivum L.; Salama & Mansour, 2015), cowpea (Vigna unguiculata L.…”

A Quantitative Profiling Method of Phytohormones and Other Metabolites Applied to Barley Roots Subjected to Salinity Stress

“…For example, Widodo et al (2009) found that the salt-tolerant barley variety Sahara, which grew better under long-term salt stress in hydroponics, showed significantly increased tricarboxylic acid (TCA) cycle intermediates and metabolites associated with cellular protection under salinity stress compared with the variety Clipper which showed drastic growth reductions. Other studies on the same barley genotypes demonstrated variety-specific effects of salinity on whole root fatty acid and lipid profiles (Natera et al, 2016), while another study detected root-zone- and variety-specific (spatial) metabolic (Shelden et al, 2016) and transcriptomic (Hill et al, 2016) signatures along longitudinal axes of roots upon salt stress.…”

Single cell‐type analysis of cellular lipid remodelling in response to salinity in the epidermal bladder cells of the model halophyte Mesembryanthemum crystallinum

“…Transcriptomic, proteomic, and metabolomic studies, including lipidomics, have shed some light on the importance of lipids in the response of plants to salt treatment, providing evidence that plant lipid metabolism is responsive to salinity at the gene, protein, and metabolite levels (Fercha et al, ; Kosová, Práil, & Vítámvás, ; Natera, Hill, Rupasinghe, & Roessner, ; Veeranagamallaiah et al, ). However, these studies have generally focused on salt‐sensitive plants.…”

“…lipid metabolism is responsive to salinity at the gene, protein, and metabolite levels (Fercha et al, 2013;Kosová, Práil, & Vítámvás, 2013;Natera, Hill, Rupasinghe, & Roessner, 2016;Veeranagamallaiah et al, 2008). However, these studies have generally focused on salt-sensitive plants.…”