Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter

Bhomkar, Prasanna; Upadhyay, Chandrama Prakash; Saxena, Mukesh; Muthusamy, Annamalai; Prakash, Neha; Pooggin, Mikhail M.; Höhn, Thomas; Sarin, Neera Bhalla

doi:10.1007/s11032-008-9164-8

Cited by 71 publications

(29 citation statements)

References 49 publications

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“…This is achieved through controlling MG levels and maintaining reduced GSH pool. Similar results were also obtained when GLYI was transformed in Vigna mungo (Bhomkar et al 2008 ). Further, over-expression of GLYII gene from rice has been carried out in different plants such as tobacco, rice, and recently in Brassica juncea, which imparts signifi cant tolerance to high MG and salt treatments similar to GLYI gene (Singla-Pareek et al 2003, 2008Wani and Gosal 2011 ;Saxena et al 2011 ).…”

Section: Role Of Glyoxalase Pathway In Plant Stress Physiologysupporting

confidence: 61%

Methylglyoxal, Triose Phosphate Isomerase, and Glyoxalase Pathway: Implications in Abiotic Stress and Signaling in Plants

Kaur

Sharma

Singla‐Pareek

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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Methylglyoxal (MG) is a cytotoxic metabolite inevitably produced as a side product of primary metabolic pathways via both enzymatic and non-enzymatic reactions. In plants, spontaneous generation of MG through breakdown of triose sugars (dihydroxyacetone phosphate and glyceraldehyde 3-phosphate) is believed to be the major route for MG formation. MG is maintained at basal levels in plants under normal conditions that accumulate to higher concentrations under various stresses, probably as a general consequence of all abiotic stresses. The toxic effects of MG is due to its ability to induce oxidative stress in cells, either directly through increased generation of reactive oxygen species (ROS) or indirectly via advanced glycation end product (AGE) formation. Thus, elevated MG levels have implications in inhibition of growth and development in plants. To keep MG levels in check, the two-step glyoxalase pathway comprising glyoxalase I (GLYI) and glyoxalase II (GLYII) enzymes has evolved as the major MG-scavenging detoxifi cation system that converts MG to D -lactate using glutathione as a cofactor in this process. Over-expression of glyoxalase pathway has been shown to confer tolerance to multiple stresses that works by controlling MG levels and maintaining glutathione homeostasis in plants. Moreover, increased activity of triose phosphate isomerase under different stresses that use up triose sugars via glycolysis further prevents MG levels from accumulating in the system along with increasing the energy status of plants. Considering the fact that MG levels are maintained at a threshold concentration in plants even under physiological conditions and also observed MG-dependent induction in expression

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Section: Role Of Glyoxalase Pathway In Plant Stress Physiologysupporting

confidence: 61%

Methylglyoxal, Triose Phosphate Isomerase, and Glyoxalase Pathway: Implications in Abiotic Stress and Signaling in Plants

Kaur

Sharma

Singla‐Pareek

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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“…They play important roles in tolerate drought, soil salinity and other abiotic stresses. According to previous studies, they have been demonstrated the high adaptation to cope with climate change or environmental stress factors for the ultimate survival of plants [150], [151].…”

Section: Discussionmentioning

confidence: 98%

Phylogenomic Analyses of Nuclear Genes Reveal the Evolutionary Relationships within the BEP Clade and the Evidence of Positive Selection in Poaceae

et al. 2013

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BEP clade of the grass family (Poaceae) is composed of three subfamilies, i.e. Bambusoideae, Ehrhartoideae, and Pooideae. Controversies on the phylogenetic relationships among three subfamilies still persist in spite of great efforts. However, previous evidence was mainly provided from plastid genes with only a few nuclear genes utilized. Given different evolutionary histories recorded by plastid and nuclear genes, it is indispensable to uncover their relationships based on nuclear genes. Here, eleven species with whole-sequenced genome and six species with transcriptomic data were included in this study. A total of 121 one-to-one orthologous groups (OGs) were identified and phylogenetic trees were reconstructed by different tree-building methods. Genes which might have undergone positive selection and played important roles in adaptive evolution were also investigated from 314 and 173 one-to-one OGs in two bamboo species and 14 grass species, respectively. Our results support the ((B, P) E) topology with high supporting values. Besides, our findings also indicate that 24 and nine orthologs with statistically significant evidence of positive selection are mainly involved in abiotic and biotic stress response, reproduction and development, plant metabolism and enzyme etc. from two bamboo species and 14 grass species, respectively. In summary, this study demonstrates the power of phylogenomic approach to shed lights on the evolutionary relationships within the BEP clade, and offers valuable insights into adaptive evolution of the grass family.

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“…Kanamycin has been successfully employed as a selectable marker in transformation of various legume crops like Vigna mungo (Karthikeyan et al 1996;Saini et al 2003;Saini and Jaiwal 2005;Bhomkar et al 2008), Jaiwal et al 2001) and Vigna angularis (Yamada et al 2001). The sensitivity of plant cells to the selective agent depends upon the genotype, the explant type, size and developmental stage of tissue(s).…”

Section: Selectionmentioning

confidence: 99%

Development of an efficient in vitro plant regeneration system amenable to Agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (Vigna mungo L. Hepper)

Sainger

Chaudhary

Dahiya

et al. 2015

Physiol Mol Biol Plants

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An efficient, rapid and direct multiple shoot regeneration system amenable to Agrobacterium-mediated transformation from primary leaf with intact petiole of blackgram (Vigna mungo) is established for the first time. The effect of the explant type and its age, type and concentration of cytokinin and auxin either alone or in combination and genotype on multiple shoot regeneration efficiency and frequency was optimized. The primary leaf explants with petiole excised from 4-day-old seedlings directly developed multiple shoots (an average of 10 shoots/ explant) from the cut ends of the petiole in 95 % of the cultures on MSB (MS salts and B 5 vitamins) medium containing 1.0 μM 6-benzylaminopurine. Elongated (2-3 cm) shoots were rooted on MSB medium with 2.5 μM indole-butyric acid and resulted plantlets were hardened and established in soil, where they resumed growth and reached maturity with normal seed set. The regenerated plants were morphologically similar to seed-raised plants and required 8 weeks time from initiation of culture to establish them in soil. The regeneration competent cells present at the cut ends of petiole are fully exposed and are, thus, easily accessible to Agrobacterium, making this plant regeneration protocol amenable for the production of transgenic plants. The protocol was further successfully used to develop fertile transgenic plants of blackgram using Agrobacterium tumefaciens strain EHA 105 carrying a binary vector pCAMBIA2301 that contains a neomycin phosphotransferase gene (nptII) and a β-glucuronidase (GUS) gene (uidA) interrupted with an intron.The presence and integration of transgenes in putative T 0 plants were confirmed by polymerase chain reaction (PCR) and Southern blot hybridization, respectively. The transgenes were inherited in Mendelian fashion in T 1 progeny and a transformation frequency of 1.3 % was obtained. This protocol can be effectively used for transferring new traits in blackgram and other legumes for their quantitative and qualitative improvements.

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Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter

Cited by 71 publications

References 49 publications

Methylglyoxal, Triose Phosphate Isomerase, and Glyoxalase Pathway: Implications in Abiotic Stress and Signaling in Plants

Methylglyoxal, Triose Phosphate Isomerase, and Glyoxalase Pathway: Implications in Abiotic Stress and Signaling in Plants

Phylogenomic Analyses of Nuclear Genes Reveal the Evolutionary Relationships within the BEP Clade and the Evidence of Positive Selection in Poaceae

Development of an efficient in vitro plant regeneration system amenable to Agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (Vigna mungo L. Hepper)

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