Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes

Youssef, Khamis; Roberto, Sérgio Ruffo; Tiepo, Angélica Nunes; Constantino, Leonel Vinícius; Resende, Juliano Tadeu Vilela de; Abo-Elyousr, Kamal A. M.

doi:10.3390/jof6030179

Cited by 32 publications

(12 citation statements)

References 46 publications

(49 reference statements)

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“…Pathogenesis-related proteins are disease defense enzymes that help control the rate of disease spread during infection (Deborah et al, 2001;Kumari and Vengadaramana, 2017). PAL, PPO, POD, and CAT are among the four crucial disease defense enzymes, and their activities are positively correlated with fruit disease resistance (Qin et al, 2015;Zhang et al, 2016;Youssef et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

et al. 2021

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To study the mechanism by which Pichia anomala induced with chitosan (1% w/v) controls blue mold disease in table grapes caused by Penicillium expansum, this study evaluated alterations in three yeast enzymatic activities. The changes in the five primary disease defense-related enzymes and two non-enzyme activities of table grapes were assayed. The results of the study showed that chitosan (1% w/v) significantly increased the yeast β-1,3-glucanase, catalase (CAT), and malondialdehyde (MDA) activities. Furthermore, P. anomala alone or induced with chitosan (1% w/v) significantly increased the table grapes enzymatic activities of Polyphenol oxidase (PPO), phenylalanine (PAL), peroxidase (POD), and catalase (CAT) compared to the control. The RT-qPCR results also confirmed that the genes of these major disease defense enzymes were up-regulated when the table grapes were treated with P. anomala. The highest results were recorded when the fruit was treated by yeast induced with chitosan (1% w/v). The phenolic compounds, in addition to their nutritional value, can also increase the antimicrobial properties of table grapes. The current experiment determined that the total phenol and flavonoid contents of table grapes showed the highest results for fruits treated by P. anomala induced with chitosan compared with the control. Generally, the increment of these fruit enzymatic and non-enzymatic activities shows improved table grape defense against the pathogenic fungus. The induction of the yeast with chitosan also increases its bio-control efficacy against the pathogen. This study will enable future detailed investigation in the yeast pathogen control mechanisms and the use of yeasts as bio-pesticides.

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Section: Discussionmentioning

confidence: 99%

“…Specific primers were obtained from Sangon Biotech (Shanghai, China). The RT-qPCR was conducted with Bio-Rad CFX96 Real-Time PCR System (Applied Biosystems, United States) and the computer program set according to Youssef et al (2020). The RT-qPCR was carried out using T.B.…”

Section: Rt-qpcr For Determination Of Defense-related Genes Expression Levelsmentioning

confidence: 99%

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

et al. 2021

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“…SOD performs dismutation of highly toxic oxygen radicals to oxygen and comparatively less toxic hydrogen peroxide. SOD activity is often increased in various biotic and abiotic stress situations, and higher activity is correlated with the resistance to the oxidative stress caused by abiotic stress as well as pathogens ( Ehsani-Moghaddam et al, 2006 ; Gill and Tuteja, 2010 ; Youssef et al, 2020 ). These enzyme activities were also reported to be modulated by phytohormones ( Szöke et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi

Khandagale

Roylawar²,

Kulkarni

et al. 2022

Front. Plant Sci.

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Purple blotch (PB) is one of the most destructive foliar diseases of onion and other alliums, caused by a necrotrophic fungal pathogen Alternaria porri. There are no reports on the molecular response of onion to PB infection. To elucidate the response of onion to A. porri infection, we consequently carried out an RNAseq analysis of the resistant (Arka Kalyan; AK) and susceptible (Agrifound rose; AFR) genotype after an artificial infection. Through differential expression analyses between control and pathogen-treated plants, we identified 8,064 upregulated and 248 downregulated genes in AFR, while 832 upregulated and 564 downregulated genes were identified in AK. A further significant reprogramming in the gene expression profile was also demonstrated by a functional annotation analysis. Gene ontology (GO) terms, which are particularly involved in defense responses and signaling, are overrepresented in current analyses such as “oxidoreductase activity,” “chitin catabolic processes,” and “defense response.” Several key plant defense genes were differentially expressed on A. porri infection, which includes pathogenesis-related (PR) proteins, receptor-like kinases, phytohormone signaling, cell-wall integrity, cytochrome P450 monooxygenases, and transcription factors. Some of the genes were exclusively overexpressed in resistant genotype, namely, GABA transporter1, ankyrin repeat domain-containing protein, xyloglucan endotransglucosylase/hydrolase, and PR-5 (thaumatin-like). Antioxidant enzyme activities were observed to be increased after infection in both genotypes but higher activity was found in the resistant genotype, AK. This is the first report of transcriptome profiling in onion in response to PB infection and will serve as a resource for future studies to elucidate the molecular mechanism of onion-A. porri interaction and to improve PB resistance in onions.

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“…Several studies recommend that there are some other alternatives to fungicides, i.e. salts against B. cinerea (Youssef et al, , 2020.…”

Section: Blue Mould (P Italicum) and Green Mould (P Digitatum) In Citrus Fruits Pome Fruits Stone Fruits Grapes And Berriesmentioning

confidence: 99%

Application of plant natural products for the management of postharvest diseases in fruits

Wan

Kahramanoğlu

Okatan

2021

Folia Horticulturae

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Prevention of postharvest losses has been a very important concern in the scientific world for many centuries, since adoption of an effective means to curtail such losses is believed to help in reaching sustainability in horticultural production and prevention of hunger around the world. The main means of deterioration in fruits, which may occur after harvest, include physiological changes/losses, physical losses, biochemical changes, changes in enzymatic activities and pathological deterioration. Among these, diseases cover the most important part; the losses due to diseases range from 5% to 20%, and this figure may extend up to >50% in the cases of certain susceptible cultivars. Fungicides have been the most important tool for the management of postharvest diseases for many years, together with hygiene, cold storage and packaging. However, due to the scientifically confirmed hazards of agro-chemicals on environment and human health, the acceptability of agro-chemicals decreased and scientists turned their attention towards natural alternatives. Most tropical and subtropical fruits contain a superficial cuticle, which helps them to regulate respiration and transpiration and protects against microbial decay. However, the waxy cuticle is generally being removed or damaged during washing or other handling practices. Therefore, the application of protective coatings (including wax) has been used in the fruit industry since the twelfth century, against microbial decay and for maintaining an acceptable standard of postharvest quality. This review aims to summarise and discuss the main natural products used for this purpose, to provide a broad-in-scope guide to farmers and the fruit storage sector.

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Salt Solution Treatments Trigger Antioxidant Defense Response against Gray Mold Disease in Table Grapes

Cited by 32 publications

References 46 publications

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

Pichia anomala Induced With Chitosan Triggers Defense Response of Table Grapes Against Post-harvest Blue Mold Disease

Comparative Transcriptome Analysis of Onion in Response to Infection by Alternaria porri (Ellis) Cifferi

Application of plant natural products for the management of postharvest diseases in fruits

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