Salt linkage formation of poly(diallyldimethylammonium chloride) with acidic groups in the polyion complex between human carboxyhemoglobin and potassium poly(vinyl alcohol) sulfate

Kokufuta, Etsuo; Shimizu, Hidetoshi; Nakamura, Isei

doi:10.1021/ma50006a008

Cited by 60 publications

(35 citation statements)

References 2 publications

(1 reference statement)

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“…Therefore, the twocomponent system in this work offers tremendous simplicity and advantages over the conventional nylon syntheses. 19,20 Controlled reactions of mono-isocyanato-azetidine-2,4-diones, 3a, have also been performed in this work. The isocyanate group of 3a is more reactive of the two functional groups, and can react readily with compounds possessing either amino, hydroxyl, or other active hydrogens.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of N‐aryl azetidine‐2,4‐diones and polymalonamides prepared from selective ring‐opening reactions

Dai

Juang

Chen

et al. 2006

J of Applied Polymer Sci

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Improved high-yield synthesis of N-aryl azetidine-2,4-dione has been achieved. The azetidine-2,4-dione undergoes ring-opening reactions with aliphatic primary amines to form malonamide linkages. More importantly, this compound exhibits a high reactivity toward primary aliphatic amine group over alcohols or secondary amines. This selective end-group functionalization is useful for preparing useful polymer intermediates. In this study polymalonamides were synthesized by fast addition reaction of aliphatic diamine and azetidine-2,4-dione. In the meantime, further application for structure-controlled reaction also has been demonstrated.

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Section: Resultsmentioning

confidence: 99%

Synthesis of N‐aryl azetidine‐2,4‐diones and polymalonamides prepared from selective ring‐opening reactions

Dai

Juang

Chen

et al. 2006

J of Applied Polymer Sci

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“…These interactions are modulated by such variables as pH and ionic strength, and may result in soluble complexes, 1,2 complex coacervation, [3][4][5][6] or the formation of amorphous precipitates. [7][8][9] Protein-polyelectrolyte complexation can change the activity of catalytic proteins (enzymes), 10 -12 alter ligand binding to transport proteins, 1,7 and stabilize biological activity against temperature change.…”

Section: Introductionmentioning

confidence: 99%

Light scattering, CD, and ligand binding studies of ferrihemoglobin-polyelectrolyte complexes

et al. 1999

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Quasi-elastic light scattering (QELS), electrophoretic light scattering (ELS), CD spectroscopy, and azide binding titrations were used to study the complexation at pH 6.8 between ferrihemoglobin and three polyelectrolytes that varied in charge density and sign. Both QELS and ELS show that the structure of the soluble complex formed between ferrihemoglobin and poly(diallyldimethylammonium chloride) [PDADMAC] varies with protein concentration. At fixed 1.0 mg/mL polyelectrolyte concentration, protein addition increases complex size and decreases complex mobility in a tightly correlated manner. At 1.0 mg/mL or greater protein concentration, a stable complex is formed between one polyelectrolyte chain and many protein molecules (i.e., an intrapolymer complex) with apparent diameter approximately 2.5 times that of the protein-free polyelectrolyte. Under conditions of excess polyelectrolyte, each of the three ferrihemoglobin-polyelectrolyte solutions exhibits a single diffusion mode in QELS, which indicates that all protein molecules are complexed. CD spectra suggest little or no structural disruption of ferrihemoglobin upon complexation. Azide binding to the ferrihemoglobin-poly(2-acrylamide-2-methylpropanesulfonate)

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“…(TABLE I), which accords with the previous report. 13 The SOD-HA binding occurs initially at pH ¼ 6.9 AE 0.1 according to Figure 3.…”

Section: Turbidimetric Titrationmentioning

confidence: 99%

“…1 Another research was done to reach industrial applications, including protein purification, [2][3][4] enzyme immobilization, 5 modulation of a protein affinity for substrates, 6 immunosensing, [7][8][9] and bioactive sensors. 10 Many techniques were applied to study the global structure of protein-polyelectrolyte complexes (PPCs), such as sedimentation, 11,12 turbidimetry, [13][14][15][16] static light scattering, [17][18][19] quasielastic light scattering (QELS), 20,21 and electrophoretic light scattering. 22 The experimental results revealed that ''primary'' soluble complexes were formed before insoluble aggregates.…”

Section: Introductionmentioning

confidence: 99%

Potentiometric studies on the interaction between superoxide dismutase and hyaluronic acid

Fan

Wang

Fan

et al. 2009

J of Applied Polymer Sci

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The formation process of soluble complexes and insoluble aggregates between superoxide dismutase (SOD) and hyaluronic acid (HA) was studied using quasielastic light scattering and turbidimetric titration. The electrostatic binding between them was investigated in detail through potentiometric titration and turbidimetric titration carried out from high to low pH. Turbidimetric titration was used to determine the specific pH values at which soluble complex formation was initiated (pH c ) and phase separation occurred (pH / ). An increase of the ionic strength causes a decrease of pH c and pH / . With the increase of HA concentrations, pH / increases but pH c does not vary. The formed ''salt bridges'' between ÀNH þ 3 (SOD) and ACOO À (HA) result in the formation of stable SOD-HA complexes and even aggregates. The necessary condition of electrostatic binding was also given for protein-acidic polyelectrolyte systems.

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Salt linkage formation of poly(diallyldimethylammonium chloride) with acidic groups in the polyion complex between human carboxyhemoglobin and potassium poly(vinyl alcohol) sulfate

Cited by 60 publications

References 2 publications

Synthesis of N‐aryl azetidine‐2,4‐diones and polymalonamides prepared from selective ring‐opening reactions

Synthesis of N‐aryl azetidine‐2,4‐diones and polymalonamides prepared from selective ring‐opening reactions

Light scattering, CD, and ligand binding studies of ferrihemoglobin-polyelectrolyte complexes

Potentiometric studies on the interaction between superoxide dismutase and hyaluronic acid

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