Salt Inhibition of Free-Living Diazotroph Population Density and Nitrogenase Activity in Soil

El-Shinnawi, M.M.; Frankenberger, W. T.

doi:10.1097/00010694-198809000-00005

Cited by 11 publications

(8 citation statements)

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“…In this case, however, nitrogen fixation in soils corresponded to the respective population count of the soils. These findings are in conformity with the earlier observations [17,24].…”

Section: Non-symbiotic Heterotrophic Diazotrophic Bacterial Counts Ofsupporting

confidence: 94%

“…This indicated that rise in soil salinity during winter had a negative effect on nitrogen fixation in soil. The effect was aggravated with further rise in soil salinity during the summer season when both diazotrophic populations and nitrogen fixation were seriously impaired, which corresponds to previous reports [17,24]. The S5 soil recorded significantly the highest value of nitrogen fixation (5.86) followed by S1 (4.52), S6 (4.29), S4 (3.77), S2 (3.76) and S3 (2.74) soils.…”

Section: Non-symbiotic Heterotrophic Diazotrophic Bacterial Counts Ofsupporting

confidence: 87%

“…Significant decrease in the diazotrophic count in the summer season corresponded to rise in salinity and depletion of available soil moisture [16,17]. The soils, collected from different sites, showed considerable variation in their diazotrophic populations.…”

Section: Non-symbiotic Heterotrophic Diazotrophic Bacterial Counts Ofmentioning

confidence: 99%

“…The negative growth of the isolates in media amended with NaCl may be due to the inhibitory action of NaCl. However, presence of the organisms in such highly saline soils may be due to the fact that the organisms are more tolerant to salinity stress in soil than in culture media [17].…”

Section: Salt Tolerance Of the Isolatesmentioning

confidence: 99%

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Studies on Non-Symbiotic Diazotrophic Bacterial Populations of Coastal Arable Saline Soils of India

et al. 2011

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The effect of fluctuations of salinity in three different seasons on diazotrophic populations and N 2 fixation in six mono cropped rice field soils of the coastal region of the Gangetic delta of West Bengal, India, was studied. The average pH, ECe, organic carbon and total nitrogen of the soils ranged from 4.99-7.08, 2.02-19.58 dSm -1 , 4.68-12.03 g kgand 0.44-1.70 g kg -1 , respectively. The average log colony forming units of the bacterial populations and N 2 -fixation in the soils varied from 4.61 to 5.86 and 2.74 to 4.52 mg N 2 fixed 50 ml -1 culture media respectively, with the lowest value recorded in summer. Recovery of microorganisms and N 2 -fixation gradually decreased with extraneous addition of NaCl in the culture media. All the eight isolates were Gram positive, spore and capsule formers. They could utilize glucose, sucrose, mannitol, starch, citrate and nitrate, and were catalase and gelatinase positive, but indole, methyl red and Vogues Proskauer reaction negative. The organisms produced alkaline reaction on TSI agar slant. The acetylene reduction assay of the isolates at 0 and 1% NaCl in the culture media were 4.51-164.52 and 1.72-100.6 nmole C 2 H 4 ml -1 culture media in 72 h, respectively. The isolates could fix 2.42-4.45 and 2.04-4.08 mg N 2 fixed 50 ml -1 culture media at 0 and 1% NaCl in the culture media respectively. 16S rDNA sequences of the isolates were similar to the species: Bacillus sp. isolate 28A, Bacillus sp. MOLA 87, Bacillus sp. By113 (B)Ydz-dh, Bacillus sp. PN13, Bacillus licheniformis strain RH101, Bacterium Antarctica 14, Bacillus sp. PN13 and Bacillus megaterium.

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“…In this case, however, nitrogen fixation in soils corresponded to the respective population count of the soils. These findings are in conformity with the earlier observations [17,24].…”

Section: Non-symbiotic Heterotrophic Diazotrophic Bacterial Counts Ofsupporting

confidence: 94%

Section: Non-symbiotic Heterotrophic Diazotrophic Bacterial Counts Ofsupporting

confidence: 87%

Section: Non-symbiotic Heterotrophic Diazotrophic Bacterial Counts Ofmentioning

confidence: 99%

Section: Salt Tolerance Of the Isolatesmentioning

confidence: 99%

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Studies on Non-Symbiotic Diazotrophic Bacterial Populations of Coastal Arable Saline Soils of India

et al. 2011

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“…This can be accomplished through the use of phosphorus (P) and K fertilizers. Soil conditions in such areas are not ideal for microbial activities (11,12). Plants absorb N from the soil mainly as NH 4 and NO 3 ions, but Dibb and Welch (7) mentioned that crops never achieve their maximum yield if they depend on NH 4 without NO 3 being present, especially in soil of dry and semi-dry areas which are fertilized by either urea or ammonium sulfate [(NH 4 ) 2 SO 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of nitrate addition on efficient use of ammonium sulfate fertilizer on corn under saline conditions. II. Field experiment

Khalifa

Zidan

2001

Communications in Soil Science and Plant Analysis

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Two field experiments during two consecutive seasons, were conducted on corn (Zea mays L. var. Ghota-82), grown on a saline soil under flood irrigation system at ACSAD research station located at the Euphrates Valley, Deir-Ez-zor District, east of Syria. The objective was to study the effect of applying different ratios of mixed NO 3 -N and NH 4 -N fertilizers on the biomass yield of corn. Five rates of nitrogen (N) (0, 50, 100, 150, and 200 kg N/ha) were applied either in a single or in combination of two forms of 15 N labelled nitrogen fertilizers ( 15 NH4) 2 SO 4 and Ca ( 15 NO 3 ) 2 Total N, 15 N, nitrogen use efficiency (N recovery), and dry matter yield were the parameters investigated. The results showed that (1) the high concentrations of NH 4 -N reduced dry matter yield; (2) NO 3 -N was more effective in increasing total N 2373

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Isolation and characterization of nitrogen‐fixing moderate halophilic bacteria from saline soils of Egypt

Zahran

Ahmad

Afkar

1995

J. Basic Microbiol.

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Twenty out of 400 isolates of bacteria mainly from salt marshes and saline soils of Egypt were successfully grown on mannitol N-free medium. The nitrogen-fixing activity was then demonstrated for the twenty isolates in modified STANIER'S medium using the acetylene reduction assay. All of them possessed appreciable nitrogenase activity (acetylene reduction) under non-saline conditions ; however, at 5% NaCl only 60% of the isolates exhibited a high rate of this activity and 25% were completely negative under these conditions. The bacterial isolates grew well in STANIER'S medium; nevertheless, growth of the majority of isolates was reduced by about 30-80% in the same medium containing 5 % NaCl. Cellulolytic activity was detected in 60% of the twenty strains, amylolytic in 45%, and pectinolytic in 10% of the isolates. The bacterial isolates showed also enzymatic activity under saline conditions (5 % NaCl). The preliminary identification indicated that six isolates were Gram positive spore-forming bacteria of the genus Bacillus, the others were Gram negative rods which remain to be identified.

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Salt Inhibition of Free-Living Diazotroph Population Density and Nitrogenase Activity in Soil

Cited by 11 publications

References 0 publications

Studies on Non-Symbiotic Diazotrophic Bacterial Populations of Coastal Arable Saline Soils of India

Studies on Non-Symbiotic Diazotrophic Bacterial Populations of Coastal Arable Saline Soils of India

Effect of nitrate addition on efficient use of ammonium sulfate fertilizer on corn under saline conditions. II. Field experiment

Isolation and characterization of nitrogen‐fixing moderate halophilic bacteria from saline soils of Egypt

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