Salt-inducible kinase 2 regulates fibrosis during bleomycin-induced lung injury

Gijsel-Bonnello, Manuel van; Darling, Nicola J.; Tsuruo, Takashi; Carmine, Samuele Di; Marchesi, Francesco; Thomson, Sarah; Clark, Kristopher; Kurowska‐Stolarska, Mariola; McSorley, Henry J.; Cohen, Philip; Arthur, J. Simon C.

doi:10.1016/j.jbc.2022.102644

Cited by 5 publications

(2 citation statements)

References 74 publications

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“…Each tube was added 7.8 mL of PBS until the total volume was 8 mL. Injection was performed intraperitoneally daily for two weeks at 2 mg.kg -1 BW [12].…”

Section: Bleomycin Preparationmentioning

confidence: 99%

Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model

Izzah,

Christina,

Dwijayanti

et al. 2024

JELS

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Pulmonary fibrosis is a chronic disease characterized by progressive connective tissue deposition that replaces healthy lung tissue. This study aimed to investigate the effect of Elephantopus scaber L. Ethanol Extract (ESEE) treatment on the relative number of IL-2 cytokine and lymphocyte activation in bleomycin (BLM)-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice were divided into seven treatment groups: N (normal); V or vehicle (corn oil); PF or Pulmonary Fibrosis (BLM 2 mg.kg -1 ); Dex (Dexamethasone 3 mg.kg -1 + BLM); D1-D3 (ESEE at doses of 0.0504, 0.1008, and 0.2016 mg.kg -1 BW + BLM). ESEE, dexamethasone, and corn oil were administered orally, followed by intraperitoneal bleomycin injection daily for 14 days. Mice were dissected on days 7 and 14, and spleens were isolated to analyze cell populations expressing CD4 + IL-2 + , CD8 + IL-2 + , CD4 + CD62L + , and CD8 + CD62L + . The results showed that bleomycin injection could increase the relative number of IL-2 and decrease the relative number of naive T cells compared to normal mice. ESEE treatment significantly reduced the relative number of IL-2, thus decreasing naive T cell activation after one week of bleomycin injection compared to the mice model. In contrast, the increased IL-2 production led to the increasing naive T cell activation after two weeks of bleomycin injection. Therefore, ESEE treatment has the potential to maintain homeostasis through modulation of IL-2 production and T-lymphocyte activation in the pulmonary fibrosis mice model.

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“…Each tube was added 7.8 mL of PBS until the total volume was 8 mL. Injection was performed intraperitoneally daily for two weeks at 2 mg.kg -1 BW [12].…”

Section: Bleomycin Preparationmentioning

confidence: 99%

Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model

Izzah,

Christina,

Dwijayanti

et al. 2024

JELS

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“…Then, 7.8 mL of PBS was added to each propylene tube. Each mice was injected with 2 mg.kg -1 BW dissolved bleomycin daily for two weeks [14]. Bleomycin was injected via the intraperitoneal route [15].…”

Section: Induction Of Pulmonary Fibrosismentioning

confidence: 99%

Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis

Nabilah,

Christina,

Dwijayanti

et al. 2024

JELS

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IL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantopus scaber L. ethanol extract (ESEE) on IL-6 and IL-17 produced by CD4 + and CD8 + in the bleomycin-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice will be divided into seven groups consisting of healthy mice (N), vehicle mice (V), pulmonary fibrosis (PF), Dexamethasone (DEX) as a drug control, and three doses of ESEE (0.0504, 0.1008, and 0.2016 mg.kg -1 BW). ESEE will be administered orally, followed by intraperitoneal bleomycin injection for 14 days. Mice are then dissected on days 7 and 14, and the spleen will be isolated for analysis of the expression of IL-6 and IL-17. The results showed that ESEE effectively reduced levels of IL-6 and IL-17 cytokines produced by CD4 + and CD8 + T cells, and doses three of ESEE (0.2016 mg.kg -1 BW) (0.2016 mg.kg -1 BW) showed the most effective reduction activity than the Dexamethasone group. The treatment was proven to reduce the expression of IL-6 and IL-17 in mice with a model of pulmonary fibrosis.

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Discovery of novel and selective SIK2 inhibitors by the application of AlphaFold structures and generative models

Zhu¹,

Liu²,

Li³

et al. 2023

Bioorganic & Medicinal Chemistry

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Salt-inducible kinase 2 regulates fibrosis during bleomycin-induced lung injury

Cited by 5 publications

References 74 publications

Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model

Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model

Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis

Discovery of novel and selective SIK2 inhibitors by the application of AlphaFold structures and generative models

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