Salt- and osmotic stress-induced choline monooxygenase expression in Kochia scoparia is ABA-independent

Kalinina, E. B.; Keith, Barbara K.; Kern, Anthony J.; Dyer, William E.

doi:10.1007/s10535-012-0132-0

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…Besides of these genes, many other genes also harbor ABA-responsive element, but they are controlled by an ABA-independent cascade ( Shinozaki et al, 2003 ). In glycinebetaine biosynthesis, the expression of CMO and BADH has also been suggested as ABA-independent, respectively ( Ishitani et al, 1995 ; Kalinina et al, 2012 ). In this study, we find that the promoter of ClBADH contains an LTR element ( Supplementary Table S5 ), which is one of the major CREs in ABA-independent gene expression ( Yamaguchi-Shinozaki and Shinozaki, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells

Sun

Jiang

et al. 2018

Front. Plant Sci.

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Glycinebetaine is an important non-toxic osmoprotectant, which is accumulated in higher plants under various stresses. The biosynthesis of glycinebetaine achieved via is a two-step oxidation from choline and betaine aldehyde, catalyzed by choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), respectively. Up-regulated gene expression of BADH and CMO induced by stress is clearly observed, but the signal transduction is poorly understood. Here, glycinebetaine accumulation in response to osmotic stress and growth recovery induced by exogenous glycinebetaine were observed in a watermelon cell line. When tracing back to the genome sequence of watermelon, it shows that there exists only one member of ClCMO or ClBADH corresponding to glycinebetaine biosynthesis. Both genes harbor a CGTCA-motif in their promoter region which is involved in methyl jasmonate (MeJA)-responsiveness. Amongst MeJA, Ethephon, abscisic acid (ABA), and salicylic acid (SA), MeJA was most effective in gene inducing the expression of ClCMO and ClBADH, and the accumulation of glycinebetaine could also reach an amount comparable to that after osmotic stress by mannitol. Moreover, when ibuprofen (IBU), a JA biosynthesis inhibitor, was pre-perfused into the cells before osmotic stress, glycinebetaine accumulation was suppressed significantly. Interestingly, newly grown cells can keep a high content of glycinebetaine when they are sub-cultured from osmotic stressed cells. This study suggests that osmotic stress induced glycinebetaine biosynthesis occurs via JA signal transduction and not only plays a key role in osmotic stress resistance but also contributes to osmotic stress hardening.

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Section: Discussionmentioning

confidence: 99%

Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells

Sun

Jiang

et al. 2018

Front. Plant Sci.

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“…ABA is a typical plant hormone associated with abiotic stress. It plays an important role in the responses to external stress signals to promote the expression of resistance-related genes [ 34 , 35 , 36 ] and regulate stomatal closure to limit water loss [ 37 ], which helps plants adapt to drought stress [ 37 , 38 ] and salt stress [ 37 , 39 , 40 ]. MtCMFs have been shown to regulate drought and salt stress either through ABA-dependent or ABA-independent mechanisms [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of BrCMF Genes in Brassica rapa and Their Expression Analysis under Abiotic Stresses

Chen,

Wu,

Zhang

et al. 2024

Plants

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CCT MOTIF FAMILY (CMF) genes belong to the CCT gene family and have been shown to play a role in diverse processes, such as flowering time and yield regulation, as well as responses to abiotic stresses. CMF genes have not yet been identified in Brassica rapa. A total of 25 BrCMF genes were identified in this study, and these genes were distributed across eight chromosomes. Collinearity analysis revealed that B. rapa and Arabidopsis thaliana share many homologous genes, suggesting that these genes have similar functions. According to sequencing analysis of promoters, several elements are involved in regulating the expression of genes that mediate responses to abiotic stresses. Analysis of the tissue-specific expression of BrCMF14 revealed that it is highly expressed in several organs. The expression of BrCMF22 was significantly downregulated under salt stress, while the expression of BrCMF5, BrCMF7, and BrCMF21 was also significantly reduced under cold stress. The expression of BrCMF14 and BrCMF5 was significantly increased under drought stress, and the expression of BrCMF7 was upregulated. Furthermore, protein–protein interaction network analysis revealed that A. thaliana homologs of BrCMF interacted with genes involved in the abiotic stress response. In conclusion, BrCMF5, BrCMF7, BrCMF14, BrCMF21, and BrCMF22 appear to play a role in responses to abiotic stresses. The results of this study will aid future investigations of CCT genes in B. rapa.

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“…Accordingly, Ah24 homologs are found in Salicornia europaea , an halophyte that is widely distributed in coastal and inland salt marshes around the world (Fan et al, 2013 ; Ma et al, 2013 ) and in various Atriplex species, many of which are used for the remediation of saline or heavy-metal contaminated soils (Kachout et al, 2010 and references therein). It is also present in exceptionally stress tolerant plants such as Kochia scoparia (Kalinina et al, 2012 and references therein), Spergularia media (syn. S. maritima ) (Flowers and Colmer, 2008 ), and Dianthus sp.…”

Section: Discussionmentioning

confidence: 99%

The novel and taxonomically restricted Ah24 gene from grain amaranth (Amaranthus hypochondriacus) has a dual role in development and defense

et al. 2015

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Grain amaranths tolerate stress and produce highly nutritious seeds. We have identified several (a)biotic stress-responsive genes of unknown function in Amaranthus hypochondriacus, including the so-called Ah24 gene. Ah24 was expressed in young or developing tissues; it was also strongly induced by mechanical damage, insect herbivory and methyl jasmonate and in meristems and newly emerging leaves of severely defoliated plants. Interestingly, an in silico analysis of its 1304 bp promoter region showed a predominance of regulatory boxes involved in development, but not in defense. The Ah24 cDNA encodes a predicted cytosolic protein of 164 amino acids, the localization of which was confirmed by confocal microscopy. Additional in silico analysis identified several other Ah24 homologs, present almost exclusively in plants belonging to the Caryophyllales. The possible function of this gene in planta was examined in transgenic Ah24 overexpressing Arabidopsis thaliana and Nicotiana tabacum plants. Transformed Arabidopsis showed enhanced vegetative growth and increased leaf number with no penalty in one fitness component, such as seed yield, in experimental conditions. Transgenic tobacco plants, which grew and reproduced normally, had increased insect herbivory resistance. Modified vegetative growth in transgenic Arabidopsis coincided with significant changes in the expression of genes controlling phytohormone synthesis or signaling, whereas increased resistance to insect herbivory in transgenic tobacco coincided with higher jasmonic acid and proteinase inhibitor activity levels, plus the accumulation of nicotine and several other putative defense-related metabolites. It is proposed that the primary role of the Ah24 gene in A. hypochondriacus is to contribute to a rapid recovery post-wounding or defoliation, although its participation in defense against insect herbivory is also plausible.

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Salt- and osmotic stress-induced choline monooxygenase expression in Kochia scoparia is ABA-independent

Cited by 9 publications

References 40 publications

Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells

Glycinebetaine Biosynthesis in Response to Osmotic Stress Depends on Jasmonate Signaling in Watermelon Suspension Cells

Genome-Wide Identification of BrCMF Genes in Brassica rapa and Their Expression Analysis under Abiotic Stresses

The novel and taxonomically restricted Ah24 gene from grain amaranth (Amaranthus hypochondriacus) has a dual role in development and defense

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