Salpingectomy increases peri-implantation endometrial HOXA10 expression in women with hydrosalpinx

Daftary, Gaurang S.; Kayisli, Umit A.; Seli, Emre; Bükülmez, Orhan; Arıcı, Aydın; Taylor, Hugh S.

doi:10.1016/j.fertnstert.2006.06.041

Cited by 102 publications

(62 citation statements)

References 35 publications

(36 reference statements)

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“…Decreased HOXA-10 gene expression level in the endometrium during the implantation window was found in infertile women with PCOS [29] and hydrosalpinx [30]. In the quoted work, gene expression normalized four months after salpingectomy.…”

Section: Discussionmentioning

confidence: 75%

Expression of HOXA-10 and HOXA-11 in the endometria of women with idiopathic infertility

Szczepańska¹,

Wirstlein²,

Łuczak³

et al. 2011

Folia Histochem Cytobiol.

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Abstract:In fertile women, HOXA-10 and HOXA-11 expression rises during the luteal phase, with the peak occurring during the implantation window, and stays at a high level until the end of the cycle. We evaluated the transcript and protein levels of HOXA-10 and HOXA-11 in the endometria of patients with idiopathic infertility (n = 15) and control patients (n = 10). The amounts of mRNA were determined by reverse transcription and real-time quantitative PCR. The protein levels were evaluated by Western blotting analysis. Using immunohistochemical techniques, we compared the localization of HOXA-10 and HOXA-11 proteins in the implantation window between the study and control groups. We observed statistically significantly decreased HOXA-10 and HOXA-11 transcript levels (p = 0.003, p = 0.012 respectively) in infertile patients compared to controls. There was no significant decrease in HOXA-10 protein levels between these groups (p = 0.074). However, we observed a significantly higher level of HOXA-11 protein in the endometria of infertile patients compared to controls (p = 0.015). HOXA-10 and HOXA-11 proteins were localized in the nuclei of the endometrial stromal cells. Immunohistochemical analyses did not reveal differences between amounts of HOXA-10 and HOXA-11 protein levels in infertility and control groups. Our results suggest that HOXA-10 and HOXA-11 gene expression in the endometrium during the implantation window may not be altered in patients with idiopathic infertility.

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Section: Discussionmentioning

confidence: 75%

Expression of HOXA-10 and HOXA-11 in the endometria of women with idiopathic infertility

Szczepańska¹,

Wirstlein²,

Łuczak³

et al. 2011

Folia Histochem Cytobiol.

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show abstract

“…Subsequently, studies on women with hydrosalpinges show that the expression of HOXA10 was significantly lower in women with hydrosalpinges compared with fertile controls. After salpingectomy, HOXA10 expression in infertile women with hydrosal-pinges was similar to that of age-matched fertile women, indicating that salpingectomy restores HOXA10 expression to physiological levels Daftary et al 2007). …”

Section: Hox Genes and Hydrosalpinxmentioning

confidence: 76%

The Role ofHoxGenes in Female Reproductive Tract Development, Adult Function, and Fertility

Taylor

2015

Cold Spring Harb Perspect Med

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196

158

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HOX genes convey positional identity that leads to the proper partitioning and adult identity of the female reproductive track. Abnormalities in reproductive tract development can be caused by HOX gene mutations or altered HOX gene expression. Diethylstilbestrol (DES) and other endocrine disruptors cause Mü llerian defects by changing HOX gene expression. HOX genes are also essential regulators of adult endometrial development. Regulated HOXA10 and HOXA11 expression is necessary for endometrial receptivity; decreased HOXA10 or HOXA11 expression leads to decreased implantation rates. Alternation of HOXA10 and HOXA11 expression has been identified as a mechanism of the decreased implantation associated with endometriosis, polycystic ovarian syndrome, leiomyoma, polyps, adenomyosis, and hydrosalpinx. Alteration of HOX gene expression causes both uterine developmental abnormalities and impaired adult endometrial development that prevent implantation and lead to female infertility.

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“…When endometrial cells are cultured in hydrosalpinx fluid, HOXA10 mRNA levels are suppressed (30). Furthermore, endometrial HOXA10 expression has been shown to normalize after removal of the hydrosalpinges (59).…”

Section: Discussionmentioning

confidence: 99%

Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity

Rackow

Taylor

2010

Fertility and Sterility

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193

143

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Study objective-To evaluate the effect of uterine leiomyomas on the endometrium using molecular markers of endometrial receptivity: HOXA10, HOXA11, LIF, and BTEB1. Design-Case-control study Setting-University medical centerPatients-Thirty reproductive-age women with submucosal, intramural, or no uterine myomas who underwent hysteroscopy or hysterectomy.Interventions-Proliferative phase endometrial sampling was performed at the time of surgery. In uteri with a submucosal myoma, directed endometrial biopsies were obtained over the myoma and over normal myometrium.Main outcome measures-Endometrial HOXA10 expression was evaluated as a primary end point using quantitative real time RT-PCR and immunohistochemistry. HOXA11, BTEB1, and LIF were evaluated using real time RT-PCR.Results-Endometrial HOXA10 and HOXA11 mRNA expression were significantly decreased in uteri with submucosal myomas compared to controls and to uteri with intramural myomas. A similar trend was seen in BTEB1 mRNA expression, however no difference was found in LIF mRNA expression. Immunohistochemistry localized the decrease in endometrial HOXA10 protein expression to stroma. In the presence of a submucosal myoma, there were no regional differences in gene expression.Conclusions-The molecular mechanism by which submucosal myomas adversely affect reproduction includes a global decrease in endometrial HOX gene expression, not simply a focal change over the myoma. This may explain the reproductive dysfunction observed with submucosal myomas.

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Salpingectomy increases peri-implantation endometrial HOXA10 expression in women with hydrosalpinx

Cited by 102 publications

References 35 publications

Expression of HOXA-10 and HOXA-11 in the endometria of women with idiopathic infertility

Expression of HOXA-10 and HOXA-11 in the endometria of women with idiopathic infertility

The Role ofHoxGenes in Female Reproductive Tract Development, Adult Function, and Fertility

Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity

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