Salmonellosis in layer chickens: pathological features and isolation of bacteria from ovaries and inner content of laid eggs

Abstract

We isolated and identified Salmonella organisms from ovaries of dead layer birds and from inner content of laid eggs of different poultry farms. The thirty eight ovarian swabs for bacteriology, visceral organs (liver, lung, spleen, egg follicles and intestine) of 38 dead birds for pathological study from 15 layer farms and 45 laid eggs (5 eggs/farm) from reported 9 Salmonella infected farms constituted samples of the study. Samples were subjected to isolation and identification of the causal agent followed by gross and histopathological study of the affected visceral organs. Seventeen of 38 ovarian swabs (44.7%) were Salmonella positive. The percentage of Salmonella Gallinarum, Salmonella Pullorum and paratyphoid causing Salmonella were 70.6%, 17.6% & 11.8%, respectively. About 53% livers of Salmonella affected birds were enlarged, congested, friable and bronze coloured with white necrotic foci. About 59% egg follicles were congested, hemorrhagic, discoloured with stalk formation and 70.6% intestines showed hemorrhagic to catarrhal enteritis. At histopathology, 76.5% livers were congested with formation of multifocal nodules and 82.4% egg follicles were congested with huge leukocytic infiltration. Infiltration of heterophils in intestinal mucosa was found in 47.1% cases. Four Salmonella isolates were found from 9 laid egg samples (5 eggs content comprised as 1 sample) and isolation rate was 44.4% reporting transovarian transmission in poultry Salmonellosis.

Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh

“…There was also 18.75% (n=3) Salmonella species were present in water samples (n=16). The results of this study were closely related with the results of several authors (Saha et al, 2012;Samanta et al, 2014).…”

Infection, colonization and shedding of Campylobacter and Salmonella in animals and their contribution to human disease: A review

“…Infections in birds by avian-adapted serovars, S. Pullorum and nonavian-adapted S. Enteritidis result in clinical disease primarily in chicks and in all ages in case of avian-adapted S. Gallinurum infections (Gast, 2013;Shivarprasad & Barrow, 2013). The faeco-oral is the main route of transmission with the exception of S. Enteritidis which can also be transmitted transovarially (Gast, 2013;Lutful Kabir, 2010;Ricke & Gast, 2014;Saha, Sufian, Hossain, & Hossain, 2012). The vertically or horizontally infected survivor chicks can remain carriers until the adult stage, infecting subsequent eggs and continuing to shed bacteria in the environment (Van Immerseel et al, 2004;Ishola, 2009;Takata, Liang, Nakano, & Yoshimura, 2003).…”

Isolation and Characterization of Salmonella Enterica Serovar Typhimurium Circulating Among Healthy Chickens of Bangladesh

“…About 44.4% of the chicken eggs are contaminated with Salmonella spp. in Bangladesh (Saha et al, 2012). Specific characterization of Salmonella isolates is therefore extremely important in order to control the Salmonella outbreak (Salehi et al, 2011;Gast, 1997).…”