Salmonella Virulence and Immune Escape

Wang, Mengyao; Qazi, Izhar Hyder; Wang, Linli; Zhou, Guangbin; Han, Hongbing

doi:10.3390/microorganisms8030407

Cited by 71 publications

(76 citation statements)

References 253 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In particular, S . Typhimurium secretes specific effector proteins, SifA or Ssel, via the SPI2-enconded T3SS, and these proteins inhibit the fusion between phagosomes and lysosomes [ 142 ]. Similar to M. tuberculosis , Neisseria gonorrhoeae PorB, the major outer membrane porin, permits Rab5 to be continuously expressed on the early phagosome, resulting in the blockade of phagosome maturation [ 143 ].…”

Section: Regulation Of Phagosome Maturation During Microbial Infecmentioning

confidence: 99%

Formation and Maturation of the Phagosome: A Key Mechanism in Innate Immunity against Intracellular Bacterial Infection

et al. 2020

View full text Add to dashboard Cite

Phagocytosis is an essential mechanism in innate immune defense, and in maintaining homeostasis to eliminate apoptotic cells or microbes, such as Mycobacterium tuberculosis, Salmonella enterica, Streptococcus pyogenes and Legionella pneumophila. After internalizing microbial pathogens via phagocytosis, phagosomes undergo a series of ‘maturation’ steps, to form an increasingly acidified compartment and subsequently fuse with the lysosome to develop into phagolysosomes and effectively eliminate the invading pathogens. Through this mechanism, phagocytes, including macrophages, neutrophils and dendritic cells, are involved in the processing of microbial pathogens and antigen presentation to T cells to initiate adaptive immune responses. Therefore, phagocytosis plays a role in the bridge between innate and adaptive immunity. However, intracellular bacteria have evolved diverse strategies to survive and replicate within hosts. In this review, we describe the sequential stages in the phagocytosis process. We also discuss the immune evasion strategies used by pathogens to regulate phagosome maturation during intracellular bacterial infection, and indicate that these might be used for the development of potential therapeutic strategies for infectious diseases.

show abstract

Section: Regulation Of Phagosome Maturation During Microbial Infecmentioning

confidence: 99%

Formation and Maturation of the Phagosome: A Key Mechanism in Innate Immunity against Intracellular Bacterial Infection

et al. 2020

View full text Add to dashboard Cite

show abstract

“…S. Enteritidis after coming in contact with human intestinal epithelial cells secretes bacterial effector proteins such as SopE, SopE2 and SopB through Salmonella Pathogenicity Island (SPI-1) encoded T3SS to influence actin cytoskeleton rearrangements for its invasion of intestinal epithelial cells [11]. S. Enteritidis induces an innate inflammatory response, diarrhea, and systemic illness after its invasion in the intestinal epithelial cells [13,14]. S. Enteritidis infection induces intestinal pro-inflammatory cytokines such as Il1β and Il8, and the resulted intestinal inflammation promotes the pathogen dissemination through macrophages [15].…”

Section: Introductionmentioning

confidence: 99%

Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion

et al. 2021

View full text Add to dashboard Cite

Salmonella Enteritidis is an intracellular foodborne pathogen that has developed multiple mechanisms to alter poultry intestinal physiology and infect the gut. Short chain fatty acid butyrate is derived from microbiota metabolic activities, and it maintains gut homeostasis. There is limited understanding on the interaction between S. Enteritidis infection, butyrate, and host intestinal response. To fill this knowledge gap, chicken macrophages (also known as HTC cells) were infected with S. Enteritidis, treated with sodium butyrate, and proteomic analysis was performed. A growth curve assay was conducted to determine sub-inhibitory concentration (SIC, concentration that do not affect bacterial growth compared to control) of sodium butyrate against S. Enteritidis. HTC cells were infected with S. Enteritidis in the presence and absence of SIC of sodium butyrate. The proteins were extracted and analyzed by tandem mass spectrometry. Our results showed that the SIC was 45 mM. Notably, S. Enteritidis-infected HTC cells upregulated macrophage proteins involved in ATP synthesis through oxidative phosphorylation such as ATP synthase subunit alpha (ATP5A1), ATP synthase subunit d, mitochondrial (ATP5PD) and cellular apoptosis such as Cytochrome-c (CYC). Furthermore, sodium butyrate influenced S. Enteritidis-infected HTC cells by reducing the expression of macrophage proteins mediating actin cytoskeletal rearrangements such as WD repeat-containing protein-1 (WDR1), Alpha actinin-1 (ACTN1), Vinculin (VCL) and Protein disulfide isomerase (P4HB) and intracellular S. Enteritidis growth and replication such as V-type proton ATPase catalytic subunit A (ATPV1A). Interestingly, sodium butyrate increased the expression of infected HTC cell protein involving in bacterial killing such as Vimentin (VIM). In conclusion, sodium butyrate modulates the expression of HTC cell proteins essential for S. Enteritidis invasion.

show abstract

“…The variation, in alleviated clinical picture of challenge among different phage types of the microbe, signified the variation in their comparative efficacy to control Salmonella enteriditis phage type 6A infection. The variation in clinical signs induced among groups inoculated with different inactivated Salmonella enteriditis phage types was understandable as there was difference in the virulence of different Salmonella enteriditis phage types [ 10 ] and hence the immunogenicity.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

Akhtar

Hair-Bejo

Hussein

et al. 2021

Veterinary Medicine International

View full text Add to dashboard Cite

This study was conducted to inactivate Salmonella enteriditis phage types (SE pt) and to determine the safety and efficacy of inactivated SE pt in chickens. SE pt 1, 3A, 6A, 7, and 35 were inactivated and inoculated (0.20 mL) in 124 chickens divided into 6 groups (CV1, CV3A, CV6A, CV7, CV35, and CV0 as a control). Sampling was conducted on day 14 after inoculation (pi). Eight chickens from each group were separated on day 14 pi for oral challenge with 0.20 mL/chicken (1010 cfu/mL) SE pt 6A and designated CV1C, CV3AC, CV6AC, CV7C, CV35C, and CV0C as control chickens. On days 7 and 14 postchallenge (pc), 4 chickens from every group were sacrificed for sampling. There was no significant difference in the body weight between different groups. In challenged groups, there was no significant association between different tissues and isolation of Salmonella on days 7 and 14 pc. There was significance (p < 0.05) in isolation of Salmonella when CV0C group was compared with other challenged groups. Significance was not observed between different tissues with respect to induction of microscopic changes. Significance was not observed between day 7 pc and day 14 pc with respect to scoring of lesions induced. Clinical signs and gross lesions were also recorded. ELISA was applied. Only in CV3AC group, the mean antibody titer was 1359 on day 14 pc. The conclusion was that inactivated SE pt 3A and 6A were safe and efficacious for protection against Salmonella enteriditis infection in chickens.

show abstract

Salmonella Virulence and Immune Escape

Cited by 71 publications

References 253 publications

Formation and Maturation of the Phagosome: A Key Mechanism in Innate Immunity against Intracellular Bacterial Infection

Formation and Maturation of the Phagosome: A Key Mechanism in Innate Immunity against Intracellular Bacterial Infection

Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion

Inactivation of Different Salmonella enteriditis Phage Types and Safety and Efficacy of Inactivated Products in Chicken

Contact Info

Product

Resources

About