Salmonella typhimurium infection in high and low antibody responder mice: inverse correlation between antibody responsiveness and resistance to infection

Abstract

Susceptibility to Salmonella typhimurium infection was compared in H (high Ab responder) and L (low Ab responder) mice obtained by several selective breeding experiments (Selections I, II, III, IV and IV A) [10,19,22]. H mice were always much more susceptible to infection than their L mice counterparts within a continuous LD 50 variation range. In three of the selections (I, II and IV A) the low responsiveness character is known to result mainly from rapid Ag degradation in L mice macrophages. It was hypothesized that resistance to multiplication of intracellular pathogens could be related to an increased catabolic activity towards Ag. This was actually demonstrated, in F2 segregant hybrids of selection IV A, by the significant inverse correlation between capacity for Ab production and resistance to infection.

Experimental visceral leishmaniasis in high and low antibody - producer mice (selection IV-A)

“…The H mice presented a higher parasite burden in spleen and liver compared to their L counterparts. This result is similar to those obtained when the lines H and L were used as experimental models for other intracellular parasites such as Leishmania tro p i c a 4 a n d Salmonella typhimurium 24 and Brucella suis 11 . In our model we observed that after 20 and 30 days H mice were more susceptible than L mice since H mice showed a significantly higher number of parasites both in spleen and liver.…”

Involvement of antibody production quantitative trait loci in the susceptibility to pristane-induced arthritis in the mouse

“…14 Outbred mice selected for high or low quantitative antibody response to Salmonella flagellar antigens 9 (Selection III) are very different in terms of antibody production to various unrelated antigens (multispecific effect), indicating that the general mechanisms of antibody synthesis have been modified in these lines. 15 High (HIII) and low (LIII) responder mice also differ in susceptibility to Salmonella enterica serotype Typhimurium infection 11 and skin tumorigenesis.…”

“…Mice bidirectionally selected for high (H) or low (L) antibody production against diverse antigens 8,9 have been important tools for the understanding of the genetic regulation of the humoral immune response and its influence in processes as diverse as susceptibility to bacterial infection, 10,11 experimental arthritis, 12 , longevity 13 and chemical tumorigenesis. 14 Outbred mice selected for high or low quantitative antibody response to Salmonella flagellar antigens 9 (Selection III) are very different in terms of antibody production to various unrelated antigens (multispecific effect), indicating that the general mechanisms of antibody synthesis have been modified in these lines.…”

Immune Response to the Rhodococcus equi Infection in High and Low Antibody‐Producing Mice (Selection IV‐A)

“…Macrophages of L mice presented high catabolism of the immunogens with consequent deficiency in their antigen presentation function leading to a low antibody production. These animals, however, have shown a higher resistance to intracellular pathogens compared to H mice whose macrophage are less catabolic, leading to a high antibody production and greater susceptibility to these pathogens (5,7,8,38).…”

“…Since R. equi is a facultative intracellular parasite, and L IV-A mice have been described as more resistant than H IV-A mice to intracellular pathogens (5,8,38,41), the Selection IV-A was considered an appropriated model to understanding innate immunity events, especially the role of activated macrophage to rhodococosis resolution. The objective of this study was to investigate immune response events and the macrophage activity of H IV-A and L IV-A mice in R. equi infection.…”