Salmonella Typhimurium exploits inflammation to its own advantage in piglets

Chirullo, Barbara; Pesciaroli, Michele; Drumo, Rosanna; Ruggeri, Jessica; Razzuoli, Elisabetta; Pistoia, Claudia; Petrucci, Paola; Martinelli, Nicola; Cucco, Lucilla; Moscati, Livia; Amadori, Massimo; Magistrali, Chiara Francesca; Alborali, G. L.; Pasquali, Paolo

doi:10.3389/fmicb.2015.00985

Cited by 22 publications

(18 citation statements)

References 34 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The three sample times at which piglets were evaluated in the current study (i.e., 2, 6, and 10 dpi), represented the acute, subacute, and recovery stages of salmonellosis, respectively. Spatially, histopathological changes were most pronounced in the distal small intestine and within the cecum and spiral colon, findings consistent with those of previous studies (26,27). It is suggested that the locations of tissue injury may be attributed to the lower concentration of bile salts and beta-defensins present at these sites (28).…”

Section: Discussionsupporting

confidence: 92%

Salmonella enterica Serovar Typhimurium Temporally Modulates the Enteric Microbiota and Host Responses To Overcome Colonization Resistance in Swine

Bescucci

Moote

Polo

et al. 2020

Appl Environ Microbiol

View full text Add to dashboard Cite

Salmonella enterica serovar Typhimurium is a prevalent incitant of enteritis in human beings and animals. It has been proposed that host defense responses incited by Salmonella allow the bacterium to overcome colonization resistance. Piglets (n=24) were orally inoculated with S. enterica Typhimurium DT104 or buffer alone, and the host and microbial responses were temporally examined at acute (2 days post-inoculation [dpi]), subacute (6 dpi), and recovery (10 dpi) stages of salmonellosis. At the acute stage of disease, body temperatures were elevated, and feed consumption and weight gain were reduced. Densities of Salmonella associated with gut mucosa decreased over time, with higher densities of the bacterium in the ileum and the large intestine. Moreover, substantive histopathologic changes were observed as a function of time, with prominent epithelial injury and neutrophil infiltration observed at 2 dpi. Correspondingly, a variety of host metrics were temporally affected in piglets with salmonellosis (e.g. TNFα, IFNγ, PR39, βD2, iNOS, IL8, REGIIIγ). The enteric microbiota was characterized using culture-independent and -dependent methods in concert, and taxon- and location-specific changes to the microbiota were observed in infected piglets. Bacteroides spp. (e.g. B. uniformis, B. fragilis), Streptococcus spp. (e.g. S. gallolyticus), and various Gammaproteobacteria were highly associated with inflamed tissues, while bacteria within Ruminococcaceae and Veillonellaceae were mainly associated with healthy mucosa. In conclusion, the study findings showed that S. Typhimurium incited temporal and spatial modifications to the swine autochthonous microbiota, and to host defense responses, that were consistent with overcoming colonization resistance to incite salmonellosis in swine. IMPORTANCE Limited information is available on host and enteric microbiota responses incited by Salmonella enterica Typhimurium in swine, and possible mechanisms by which the bacterium overcomes colonization resistance to incite salmonellosis. Temporal characterization of a variety of host metrics in piglets (e.g. physiologic, histopathologic, and immunologic) showed the importance of studying the progression of salmonellosis. A number of host responses integrally associated with disease development were identified. Utilization of next-generation sequence analysis to characterize the enteric microbiota was found to lack sufficient resolution; however, culture-dependent and -independent methods in combination identified taxon- and location-specific changes to bacterial communities in infected piglets. The study identified bacteria and host responses associated with salmonellosis, which will be beneficial in understanding colonization resistance and for the development of effective alternatives to antibiotics to mitigate salmonellosis.

show abstract

Section: Discussionsupporting

confidence: 92%

Salmonella enterica Serovar Typhimurium Temporally Modulates the Enteric Microbiota and Host Responses To Overcome Colonization Resistance in Swine

Bescucci

Moote

Polo

et al. 2020

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Indeed, in our study we showed up-regulation of the pro-inflammatory cytokines IL6 and IL8, given in accordance with Salmonella's ability to penetrate D-17; in fact it is known as this microorganism exploits inflammation to penetrate enterocytes [8]. This confirms as D-17 can interact with an infectious stressor such as Salmonella typhimurium, suggesting D-17 as a useful cell line for the preliminary evaluation of new bacteria-based therapeutic approaches [51].…”

Section: Discussionsupporting

confidence: 83%

Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy

Modesto

Fernandez²,

Martini

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

Osteosarcoma (OSA) is a rare cancer both in human and dog although the incidence rate in dogs is 27 times higher than in human. Many studies employed D-17 as cell line for in vitro test to evaluate conventional anticancer therapies; however, little is known about D-17 cell line. The aim of our study was to evaluate the basal level of gene expression of pivotal molecules in the innate immune response and cell cycle regulation and to establish the ability of this cell line to react to Salmonella typhimurium (ST) infective stressor. IL15, IL10, iNOS, TLR5, CD14, PTEN and IL18 were expressed in an inconsistent manner among experiments. The other genes under study were expressed in all samples. ST showed ability to penetrate D-17 causing pro-inflammatory response. Our results outline the expression in D-17 of important genes involved in innate immune response. These results provide important data on D-17 basal gene expression profile useful for in vitro preliminary evaluation of new therapeutic approaches.

show abstract

“…It is known that, similarly to what happens in vitro and in murine models of infection (Barthel et al, 2003 ; Stecher et al, 2007 ; Barman et al, 2008 ), S. Typhimurium strains induce an acute inflammatory response in the intestinal mucosa also in piglets (Bearson et al, 2013 ). Several studies have proved how S. Typhimurium takes advantages of inflammation to compete with the resident microbiota and to colonize the inflamed gut in mice (Lupp et al, 2007 ; Stecher et al, 2007 ; Barman et al, 2008 ; Winter et al, 2010 ) and piglets (Chirullo et al, 2015 ). In our study, we investigated the impact of S. Typhimurium on the porcine intestinal microbial communities.…”

Section: Discussionmentioning

confidence: 99%

Salmonella enterica Serovar Typhimurium Exploits Inflammation to Modify Swine Intestinal Microbiota

Drumo

Pesciaroli

Ruggeri

et al. 2016

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

Salmonella enterica serovar Typhimurium is an important zoonotic gastrointestinal pathogen responsible for foodborne disease worldwide. It is a successful enteric pathogen because it has developed virulence strategies allowing it to survive in a highly inflamed intestinal environment exploiting inflammation to overcome colonization resistance provided by intestinal microbiota. In this study, we used piglets featuring an intact microbiota, which naturally develop gastroenteritis, as model for salmonellosis. We compared the effects on the intestinal microbiota induced by a wild type and an attenuated S. Typhimurium in order to evaluate whether the modifications are correlated with the virulence of the strain. This study showed that Salmonella alters microbiota in a virulence-dependent manner. We found that the wild type S. Typhimurium induced inflammation and a reduction of specific protecting microbiota species (SCFA-producing bacteria) normally involved in providing a barrier against pathogens. Both these effects could contribute to impair colonization resistance, increasing the host susceptibility to wild type S. Typhimurium colonization. In contrast, the attenuated S. Typhimurium, which is characterized by a reduced ability to colonize the intestine, and by a very mild inflammatory response, was unable to successfully sustain competition with the microbiota.

show abstract

Salmonella Typhimurium exploits inflammation to its own advantage in piglets

Cited by 22 publications

References 34 publications

Salmonella enterica Serovar Typhimurium Temporally Modulates the Enteric Microbiota and Host Responses To Overcome Colonization Resistance in Swine

Salmonella enterica Serovar Typhimurium Temporally Modulates the Enteric Microbiota and Host Responses To Overcome Colonization Resistance in Swine

Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy

Salmonella enterica Serovar Typhimurium Exploits Inflammation to Modify Swine Intestinal Microbiota

Contact Info

Product

Resources

About