Salmonella typhi iron-regulated outer-membrane proteins cause oedema and hyperalgesia during inflammation induced in a rat model

Chanana, Vishal; Sehgal, Rakesh; Rishi, Praveen

doi:10.1099/jmm.0.45907-0

Cited by 10 publications

(12 citation statements)

References 15 publications

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“…5 ìg of the protein was observed to enhance the DTH response significantly. This is in contrast to a 50 ìg dose of the same antigen as discussed recently (Chanana et al, 2005) or of porins extracted under normal conditions (Singh et al, 1999). Since organisms under environmental stresses have been reported to express more virulence determinants, lower concentrations of bacteria/ bacterial products might be required.…”

Section: Dth Responsementioning

confidence: 81%

“…These proteins were resolved on SDS-PAGE. The IROMPs preparation showed three bands in the molecular mass range of 66-97 kDa as reported recently (Chanana et al, 2005). IROMPs eluted from the gel (Hager & Burgess, 1980) were further purified by HPLC (Protein pack SW 125) as described earlier (Sood et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

“…S. Typhi has been reported to express iron-uptake systems characterized by secreted siderophores along with the expression of certain proteins on the cell surface referred to as iron-regulated outer-membrane proteins (IROMPs). The environment of the host has been reported to regulate the in vivo expression of IROMPs, and recently, these proteins have been shown to have inflammatory potential (Chanana et al, 2005). These proteins have been considered to be promising vaccine candidates for a number of Gram-negative bacteria including Haemophilus influenzae, Helicobactor pylori and Neisseria species (Schryvers, 1989;Banerjee-Bhatnagar & Frasch, 1990;Worst et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Sood

Rishi

Vohra

et al. 2005

Journal of Medical Microbiology

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Section: Dth Responsementioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Sood

Rishi

Vohra

et al. 2005

Journal of Medical Microbiology

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“…Rocha et al [30] have shown that response to oxidative stress in anaerobically grown B. fragilis induces the synthesis of at least 28 proteins in the presence of H 2 O 2 that confers protection to the organism against the hydrogen peroxide induced oxidative damage. Pathogens have been reported to combat these stress conditions through the induction of various iron-regulated proteins [31,16], oxidative stress proteins [19,32] and anaerobic stressed proteins [33]. However, the coordination of phenotype(s) and their immunodominance under iron and oxygen availability have not been studied in detail.…”

Section: Discussionmentioning

confidence: 99%

“…There is a vast amount of information about OMPs and their roles in virulence, inflammation, immunogenicity and anti-microbial resistance [14][15][16]. However, the knowledge about the expression of OMPs of the bacteria, in reference to coordination of iron and oxygen availability is largely not available.…”

Section: Introductionmentioning

confidence: 99%

Coordinated expression and immunogenicity of an outer membrane protein from Salmonella enterica serovar Typhi under iron limitation, oxidative stress and anaerobic conditions

et al. 2005

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SummarySuccessful pathogens overcome the environmental stresses by the coordinated expression of various genes and eventually proteins. Since, the surface of the microbe is likely to come in contact with the host initially, an attempt was made to identify the outer membrane proteins (OMPs), if any, which may get expressed under more than one environmental conditions simulating the in vivo ones. In the present study, Salmonella enterica serovar Typhi was grown under iron-limited, oxidative stress as well as anaerobic conditions and the OMP profiles were compared. A 69 kDa OMP was found to express with enhanced intensity under the selected stress conditions in comparison to normal conditions. The phenotypic similarity among the proteins was assessed on the basis of their molecular weight, cross reactivity and HPLC. The protein expressed under oxidative stress and anaerobic conditions reacted with the antibodies raised against iron-regulated outer membrane protein (IROMP), indicating the sharing of at least some of the epitopes. A single peak observed after subjecting the pooled 69 kDa protein sample and appearance of a single band on SDS-PAGE thereafter, confirmed the purity and phenotypic similarity of the 69 kDa OMP. Reactivity of pooled 69 kDa protein with 85% of sera from typhoid patients revealed the in vivo expression of this protein. The results of this study indicate the coordination of this phenotype under iron stress, oxidative stress and anaerobic conditions. In view of the expression of the 69 kDa protein under the selected stress conditions and their in vivo immunogenicity, these findings may be relevant for the better understanding of the host-microbe interactions and for the further development of diagnostic and preventive strategies.

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Phytomodulatory potentials of Aloe vera against Salmonella OmpR‐mediated inflammation

Rishi

Rampuria

Tewari

et al. 2008

Phytotherapy Research

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Mediators released during inflammatory response play an essential role in eliminating microbes or microbial products. However, the uncontrolled release of cytotoxic substances characterized by extensive inflammation may adversely affect normal tissues. Under such conditions it is important to manage the hyperinflammation in order to change the clinical manifestations of the disease. Accordingly, the present study was designed to evaluate the modulation of Salmonella OmpR mediated inflammation by Aloe vera, a plant known to contain antiinflammatory ingredients. It was observed that outer-membrane proteins (OMPs) extracted from the wild type strain of S. typhimurium caused inflammation of greater magnitude compared with the OMPs extracted from its mutant construct as evident from the oedema test as well as the hyperalgesic (flicking) response of the animals under experimental conditions. However, Aloe vera applied topically, administered intraperitoneally or in combination modulated the inflammatory response. The maximum effect was observed with the combined formulation indicating modulation at local as well as systemic levels. The results reveal that this modulation could be due to the potential of Aloe vera to decrease peroxidative damage via a decrease in the levels of monokines (TNF-alpha, IL-1 and IL-6) and an increase in the level of superoxide dismutase (SOD). Moreover, the presence of SOD in Aloe vera itself might be responsible for enhancing its levels in the macrophages. On the other hand, no significant change in the catalase activity was observed by Aloe vera treatment. The use of Aloe vera, therefore, seems to have a promising role in the modulation of Salmonella OmpR mediated inflammation.

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Salmonella typhi iron-regulated outer-membrane proteins cause oedema and hyperalgesia during inflammation induced in a rat model

Cited by 10 publications

References 15 publications

Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Cellular immune response induced by Salmonella enterica serotype Typhi iron-regulated outer-membrane proteins at peripheral and mucosal levels

Coordinated expression and immunogenicity of an outer membrane protein from Salmonella enterica serovar Typhi under iron limitation, oxidative stress and anaerobic conditions

Phytomodulatory potentials of Aloe vera against Salmonella OmpR‐mediated inflammation

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