Salmonella surveillance on fresh produce in retail in Turkey

Gunel, Elif; Kilic, Gozde Polat; Bulut, Ece; Durul, Bora; Acar, Sinem; Alpas, Hami; Soyer, Yeşim

doi:10.1016/j.ijfoodmicro.2015.01.010

Cited by 22 publications

(10 citation statements)

References 28 publications

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“…ST26 was the most common especially among poultry samples and this ST corresponded to S. Thompson that has a low rate of occurrence in China (Ni et al, 2018). ST11 and ST19 were the most frequent sequence types recovered from meat samples and these STs are commonly associated with human salmonellosis outbreaks (Gunel et al, 2015; Ashton et al, 2016). Additionally, our results revealed that the serotypes and STs were highly correlated, which is consistent with previous research (Achtman et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Incidence and Characterization of Salmonella Isolates From Raw Meat Products Sold at Small Markets in Hubei Province, China

Zhou

Hou

et al. 2019

Front. Microbiol.

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Salmonella is a leading cause of foodborne disease and is often associated with the consumption of foods of animal origin. In this study, sixty-six Salmonella isolates were obtained from 631 raw meat samples purchased at small retail suppliers in Hubei Province, China. The most prevalent Salmonella serotypes were Thompson (18.2%) and Agona (13.6%). Frequent antimicrobial resistance was observed for the sulfonamides (43.9%), tetracycline (43.9%), and the β-lactams amoxicillin and ampicillin (36.4% for each). Interestingly, a high incidence of resistance to cephazolin was observed in strains of the most common serotype, S. Thompson. Class I integrons were found in 27.3% (18/66) of the isolates and five of these integrons contained different gene cassettes (aacA4C-arr-3-dfr2, dfrA12-aadA21, aadA2, dfrA12-aadA2, dfr17-aadA5). Additional antimicrobial resistance genes, including blaTEM–1, blaCTX–M–65, blaCTX–M–15, qnrB, and qnrS, were also identified among these Salmonella isolates. Results of replicon typing and conjugation experiments revealed that an integron with qnrB and blaCTX–M–15 genes was present on incH12 mobile plasmid in S. Thompson strain. Multilocus sequence typing (MLST) analysis revealed 32 sequence types, indicating that these isolates were phenotypically and genetically diverse, among which ST26 (18.2%) and ST541 (12.1%) were the predominant sequence types. The integrons, along with multiple antimicrobial resistance genes on mobile plasmids, are likely contributors to the dissemination of multidrug resistance in Salmonella.

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Section: Resultsmentioning

confidence: 99%

Incidence and Characterization of Salmonella Isolates From Raw Meat Products Sold at Small Markets in Hubei Province, China

Zhou

Hou

et al. 2019

Front. Microbiol.

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“…PFGE is an efficient sub-typing method and commonly used in outbreak investigations. PFGE produces comparable data of genotypic characteristics of Salmonella strains and it is accepted as the gold standard among molecular methods [12,13]. In addition to this, it has been used in typing Salmonella in human patients, animal sources and foods because of its discriminatory power and high reproducibility [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of food originated Salmonella isolates

Karatuğ

Yüksel

Akçelik

et al. 2018

Biotechnology & Biotechnological Equipment

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Currently, Salmonella enterica is the most common bacterial foodborne pathogen, causing serious extraintestinal disease. Typing methods play an important role on pathogens' source tracking, knowing the source(s) of bacteria in pharmaceutical sciences, preventing and controlling the diarrhea and food-poisoning outbreaks. The purpose of this study is to use different moleculer typing methods to determine the genetic variability of 38 foodborne Salmonella isolates that were previously identified by biochemical tests. The methods were evaluated by four molecular techniques including 16S rRNA sequencing, PFGE, PCR-RFLP and invA-spvC PCR. 16S rRNA sequencing results showed that four of the 38 isolates were Escherichia coli, Proteus mirabilis and Citrobacter murliniae, and the others were Salmonella enterica. Thirty-eight strains were subtyped by XbaI-PFGE into 20 profiles with different clusters, while they were subtyped by 16S rRNA-RFLP into 9 profiles with a single cluster. Out of two Salmonella isolates, the invasion gene (invA) was detected in all other Salmonella isolates (94%) and the virulence gene (spvC) was detected in 11% of Salmonella isolates. Our results suggested that the PFGE subtyping is the prominent method for the evaluation and benchmarking of molecular subtyping.

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“…In this study, we used different Salmonella isolates, representing most common serovars associated with foodborne outbreaks bound up with fresh produces, as well as serovars we collected from fresh produces previously in our laboratory (Gunel et al, 2015;Acar et al, 2017). Salmonella Virchow, collected from chicken meat, was the only multidrug-resistant (resistant to ampicillin, ceftiofur, nalidixic acid, streptomycin, sulfisoxazole, trimethoprim, and sulfamethoxazole-trimethoprim) serovar used in this study.…”

Section: Resultsmentioning

confidence: 99%

“…Salmonella enterica serovar Virchow (MET S1-003), Newport (MET S1-166), Typhimurium (MET S1-185), Enteritidis (MET S1-217), Othmarschen (MET S1-227) and Mikawasima (MET S1-409), isolated from different sources and locations in our previous studies (Gunel et al, 2015;Acar et al, 2017), were used in this study (Table 1). Each serovar was grown in Brain Heart Infusion (BHI, CM1135 Oxoid) broth medium for 24 h at 37°C at 150 rpm to get the stationary phase cultures (Cui et al, 2016).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Isirgan Otu (Urtica Dioica) Sulu Ekstraktinin Salmonella Enterica Serovarlarinin Bi̇yofi̇lm Oluşumu Üzeri̇ne Anti̇mi̇krobi̇yel Etki̇si̇ni̇n Beli̇rlenmesi̇

Cesur

Soyer

2021

Gıda

Self Cite

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Due to health concerns associated with the increase of antimicrobial resistance in foodborne pathogens such as Salmonella, plant extracts have become important natural alternatives to known antimicrobials. The eradication effect of the water-soluble stinging nettle extracts with 2 mg/mL, 4 mg/mL, 6 mg/mL, 8 mg/mL, 12 mg/mL, 16 mg/mL, and 20 mg/mL concentrations, on pre-formed biofilms and swimming motility of Salmonella enterica subspecies enterica serovars, including Newport, Typhimurium, Enteritidis, Virchow, Othmarschen and Mikawasima, was investigated in vitro. Degradation of biofilm formation on spinach inoculated with each serovar was ascertained within different exposure time of 40 mg/mL concentrated extract as well. Moreover, major genes responsible for biofilm formation (i.e., rpoS, mlrA, ycfR, fimA, spiA and csgA) were screened in these isolates. The extract significantly decreased swimming motilities of Mikawasima and Virchow serovars. The highest reductions were found as 0.88 Log CFU/mL and 2.00 Log CFU/cm 2 in vitro and on spinach, respectively.

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Salmonella surveillance on fresh produce in retail in Turkey

Cited by 22 publications

References 28 publications

Incidence and Characterization of Salmonella Isolates From Raw Meat Products Sold at Small Markets in Hubei Province, China

Incidence and Characterization of Salmonella Isolates From Raw Meat Products Sold at Small Markets in Hubei Province, China

Genetic diversity of food originated Salmonella isolates

Isirgan Otu (Urtica Dioica) Sulu Ekstraktinin Salmonella Enterica Serovarlarinin Bi̇yofi̇lm Oluşumu Üzeri̇ne Anti̇mi̇krobi̇yel Etki̇si̇ni̇n Beli̇rlenmesi̇

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