Salmonella spp. and Escherichia coli O157:H7 prevalence and levels on lettuce: A systematic review and meta-analysis

Elias, Susana de Oliveira; Noronha, Tiago Baptista; Tondo, Eduardo César

doi:10.1016/j.fm.2019.05.001

Cited by 46 publications

(22 citation statements)

References 37 publications

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“…According to the World Health Organization (WHO, 2015), every year, about 600 million cases of foodborne illnesses and 420 000 associated deaths occur worldwide (Hoelzer, Switt, Wiedmann, & Boor, 2018). Among the microorganisms most frequently associated with foodborne outbreaks, Salmonella enterica and Escherichia coli play an important role (de Oliveira Elias, Noronha, & Tondo, 2019). Salmonellosis, the illness caused by Salmonella, include symptoms such as diarrhea, fever and abdominal pain (CDC, 2018).…”

Section: Introductionmentioning

confidence: 99%

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Alves

Cerqueira

Pastrana

et al. 2020

Food Research International

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Notwithstanding the implementation of good processing practices in food companies and appropriate washing of food products by the consumer, Salmonella and Escherichia coli outbreaks continue to occur. In this study, different combinations of bacteriophages (phages) and cinnamaldehyde (CNMA) were incorporated on sodium alginate emulsion-based films to impart them with antimicrobial activity towards S. Enteritidis and E. coli. Films were prepared by casting and they were characterized in terms of CNMA and/or phages loading, thickness, moisture content, water vapor permeability (WVP), swelling index (SW), chemical interactions by FTIR, surface morphology by SEM and antimicrobial performance. Results showed that phages incorporation was not compromised by CNMA as evidenced by their viability inside the films. Increasing CNMA concentration yielded formulations less heterogeneous and a higher amount of CNMA loaded. Films characterization revealed that, in general, phages incorporation did not introduce significant changes on films parameters while the presence of CNMA increased the roughness, thickness and swelling ability of films. Sodium alginate films incorporated with EC4 and φ135 phages displayed antimicrobial activity against E. coli and S. Enteritidis, respectively, while CNMA empowered the films with activity against both species. Combination of both phages with the higher concentration of CNMA resulted in a synergic antimicrobial effect against E. coli and a facilitative effect against Salmonella. Overall, incorporation of EC4 and φ135 phages together with CNMA on alginate emulsion-based films holds great potential to be further applied in food packaging to prevent food contamination.

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Section: Introductionmentioning

confidence: 99%

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Alves

Cerqueira

Pastrana

et al. 2020

Food Research International

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“…5b). The harm caused by foodborne pathogens has become a serious challenge to humans [45]. Researchers began to use phages as natural antimicrobials in food to kill or inhibit foodborne pathogens, thus ensuring food safety [46,47].…”

Section: Killing Of Phb08 In Vitromentioning

confidence: 99%

Isolation of a lytic bacteriophage vB_EfaS_PHB08 and its endolysin lys08 against Enterococcus faecalis biofilm

Yang

Chen

Sun

et al. 2020

Preprint

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Background: Enterococcus faecalis is an opportunistic pathogen can cause a variety of diseases, such as urinary tract infections and wound infections in human and animals. Recently, bacteriophages and their derivatives represent a good effect on fighting against bacterial infections. Methods: We isolated virulent bacteriophages of E. faecalis using the double-layer plate method. The bio-activities of the phage isolated were determined via one-step growth curve testing and bacterial killing assays. Illumina HiSeq sequencing was performed to determine the genetic characteristics and the lysins of the phage. Protein expression and antibiofilm assays were also performed to highlight the bio-activities of the phage lysins. Results: We isolated a virulent bacteriophage vB_EfaS_PHB08 (thereafter PHB08) from the sewage nearby hospital. PHB08 possessed a linear double-stranded DNA genome with 55,244 bp in length, which encoded 91 putative coding sequences (CDS). We found that PHB08 could inhibit the growth of host bacteria for 12 h. In vegetable models, PHB08 can reduce 1´105 Colony Forming Units (CFU) of E. faecalis per square centimeter at room temperature (25 °C) for 24 h. In addition, PHB08 and its endolysin can remove the biofilm formed by E. faecalis. Conclusions: A virulent phage and endolysin displayed a good effect on reducing and/or eradicating E. faecalis infection and biofilm.

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“…In 2016, a total of two Salmonella outbreaks in Australia have been attributed to fresh produce and Salmonella Anatum outbreak (144 cases) linked to prepackage lettuce and Salmonella Hvittingfoss (97 cases) linked to rock melons [ 2 ]. S almonella contamination occurs during both production and preparation phases by exposure to contaminated water, soil, insect, or animal fecal matter and through cross-contamination [ 3 ]. Animal manure used as fertilizers is a potential source of contamination of raw vegetables [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…This has led to cross-contamination, growth, and exposure to and illness from Salmonella of chicken origin [ 5 ]. A systematic review of Salmonella contamination of lettuce indicated that Salmonella prevalence in lettuce was 2.1, 1.0, and 16.9% for farm, industry, and retail markets, respectively [ 3 ]. These pose a serious threat to the public health.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Stress Tolerance of Salmonella with Different Antibiotic Resistance Profiles

Xiao

Tang

Liu

et al. 2021

BioMed Research International

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Disease caused by antibiotic-resistant Salmonella is a serious clinical problem that poses a great threat to public health. The present study is aimed at assessing differences in bacterial kinetics with different antibiotic resistance profiles under environmental stress and at developing microbial tolerance models in lettuce during storage from 4 to 36°C. The drug-resistance phenotypes of 10 Salmonella Typhimurium (S. Typhimurium) isolates were examined using the broth microdilution method. The results of 10 S. Typhimurium isolates in the suspensions showed that a slow trend towards reduction of drug-sensitive (DS) isolates in relation to the others though without statistical difference. Compared to DS S. Typhimurium SA62, greater bacterial reduction was observed in multidrug-resistant (MDR) S. Typhimurium HZC3 during lettuce storage at 4°C ( P < 0.05 ). It was likely that a cross-response between antibiotic resistance and food-associated stress tolerance. The greater growth in lettuce at 12°C was observed for DS S. Typhimurium SA62 compared to MDR S. Typhimurium HZC3 and was even statistically different ( P < 0.05 ), while no significant difference was observed for bacterial growth between MDR S. Typhimurium HZC3 and DS S. Typhimurium SA62 strains in lettuce storage from 16 to 36°C ( P > 0.05 ). The goodness-of-fit indices indicated the Log-linear primary model provided a satisfactory fit to describe the MDR S. Typhimurium HZC3 and DS S. Typhimurium SA62 survival at 4°C. A square root secondary model could be used to describe the effect of temperature (12, 16, 28, and 36°C) on the growth rates of S. Typhimurium HZC3 ( a d j − R 2 = 0.91 , RMSE = 0.06 ) and S. Typhimurium SA62 ( a d j − R 2 = 0.99 , RMSE = 0.01 ) derived from the Huang primary model. It was necessary to pay attention to the tolerance of antibiotic resistant bacteria under environmental stress, and the generated models could provide parts of the input data for microbial risk assessment of Salmonella with different antibiotic resistance profile in lettuce.

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Salmonella spp. and Escherichia coli O157:H7 prevalence and levels on lettuce: A systematic review and meta-analysis

Cited by 46 publications

References 37 publications

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Entrapment of a phage cocktail and cinnamaldehyde on sodium alginate emulsion-based films to fight food contamination by Escherichia coli and Salmonella Enteritidis

Isolation of a lytic bacteriophage vB_EfaS_PHB08 and its endolysin lys08 against Enterococcus faecalis biofilm

Evaluation of the Stress Tolerance of Salmonella with Different Antibiotic Resistance Profiles

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