Salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence

Fu, Jiaqi; Qi, Linlu; Hu, Mo; Liu, Yanhua; Yu, Kaiwen; Liu, Qian; Liu, Xiaoyun

doi:10.1016/j.jprot.2017.02.004

Cited by 26 publications

(25 citation statements)

References 37 publications

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Section: Discussionmentioning

confidence: 61%

“…Although both Nap and Nar systems belong to dissimilatory nitrate reductases, the Nar system is strictly involved in redox regulation and energy acquisition, whereas the Nap system is functionally diverse [30]. Both nitrate reductases, Nap and Nar, are membrane associated, whereas Nas is made of soluble proteins located in the cytoplasm [38]. We showed that the STY1099 protein only affects membrane associated nitrate reductase, which is further supported by the fact that this novel protein is associated with cellular membrane (i.e., monopolar localization), but not with cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

“…Fu and colleagues [38], examining response of S. enterica Typhimurium to H 2 O 2 treatment, found that this Salmonella serovar significantly induced several proteins associated with DNA repair machinery, including RecA, RecE and GyrI. The current study showed that the STY1099 protein interacted with DNA-binding ATP-dependent protease La, showing that this novel protein is indirectly associated with DNA repair machinery, an important response of Salmonella enterica to peroxide stress [38]. Besides DNA-binding proteases La, the STY1099 protein interacted with key protease, ClpX, chaperone, DnaK, and the two-component response regulator, OmpR, which controls a variety of membrane-associated transporters that mediate uptake or efflux of small molecules [39], showing its importance in the stress response physiology.…”

Section: Discussionmentioning

confidence: 99%

“…The overall average pairwise distance among the three families, Enterobacteriaceae, Vibrionaceae and Pasteurellaceae, was 0.437, indicating any two bacterial strains from this group would diverge, on average, 43.7% from each other based on the yccT gene sequence. Alignment of the STY1099 protein sequence revealed numerous conserved domains, including amino acid positions, 1, 2, 21,34,38,42,62,83,90,103,135,137,155,156,162,200,203,206,210, 211, 214 and 218 ( Figure 1B). The STY1099 protein sequences, from this diverged group of bacteria, exhibited common binding sites with high pocket multiplicity (PM) values for SO 4 (PM 20), Zn + (PM 20), Na + (PM 9) and HO (PM 9).…”

Section: Phylogeny Conserved Domains and Structural Characteristics mentioning

confidence: 99%

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Transcriptional Profiling and Molecular Characterization of the yccT Mutant Link: A Novel STY1099 Protein with the Peroxide Stress Response and Cell Division of Salmonella enterica Serovar Enteritidis

Vidović

An³

et al. 2019

Biology

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Uncharacterized protein STY1099, encoded by the yccT gene, was previously identified as the most altered (i.e., upregulated) protein among the ZnO nanoparticle (NP) stimulon of Salmonella enterica serovar Enteritidis. Here we combined various stress response-related assays with functional genetics, global transcriptomic and proteomic analyses to characterize the yccT gene and its STY1099 product. Exposure of S. enterica Enteritidis to H2O2 (i.e., hydrogen peroxide) resulted in a significant (p < 0.0001) upregulation of the yccT gene, whereas exposure to paraquat (i.e., superoxide) did not alter the expression of the yccT gene. The ∆yccT mutant of S. enterica Enteritidis exposed to 0.75 mM H2O2, showed significantly reduced (p < 0.05) viability compared to the wild type strain. Further, comparative transcriptome analyses supported by Co-immunoprecipitation (Co-IP) assay revealed that STY1099 protein plays a role in redox homeostasis during the peroxide stress assault via involvement in the processes of respiratory nitrate reductase, oxidoreductase activities, cellular uptake and stress response. In addition, we found that the STY1099 protein has the monopolar subcellular location and that it interacts with key cell division proteins, MinD, and FtsH, as well as with a rod shape-determining protein MerB.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Phylogeny Conserved Domains and Structural Characteristics mentioning

confidence: 99%

See 2 more Smart Citations

Transcriptional Profiling and Molecular Characterization of the yccT Mutant Link: A Novel STY1099 Protein with the Peroxide Stress Response and Cell Division of Salmonella enterica Serovar Enteritidis

Vidović

An³

et al. 2019

Biology

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“…Accumulation of ROS and endothelial redox imbalance have been widely demonstrated in the atherothrombotic plaque in vitro, in animal models and in human vascular tissues 43,65 . Oxidative stress is up-regulated upon infection by Escherichia coli or Salmonella typhimurium [66][67][68] . Conversely, in vitro models indicate that eNOS endothelial activity is reduced upon TNF-α or LPS incubation 69 whereas experimental bacterial meningitis in engineered mice with iNOS invalidation support the hypothesis of a dual endothelial iNOS and eNOS up-regulation upon comparison with wild type individuals 70 .…”

Section: Discussionmentioning

confidence: 99%

Porphyromonas gingivalis triggers the shedding of inflammatory endothelial microvesicles that act as autocrine effectors of endothelial dysfunction

Bugueno

El-Ghazouani

Batool

et al. 2020

Sci Rep

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A link between periodontitis and atherothrombosis has been highlighted. The aim of this study was to determine the influence of Porphyromonas gingivalis on endothelial microvesicles (EMV pg) shedding and their contribution to endothelial inflammation. Endothelial cells (EC) were infected with P. gingivalis (MOI = 100) for 24 h. EMV pg were isolated and their concentration was evaluated by prothrombinase assay. EMV pg were significantly increased in comparison with EMV ctrl shedded by unstimulated cells. While EMV ctrl from untreated EC had no effect, whereas, the proportion of apoptotic EC was increased by 30 nM EMV pg and viability was decreased down to 25%, a value elicited by P. gingivalis alone. Moreover, high concentration of EMV pg (30 nM) induced a pro-inflammatory and pro-oxidative cell response including up-regulation of TNF-α, IL-6 and IL-8 as well as an altered expression of iNOS and eNOS at both mRNA and protein level. An increase of VCAM-1 and ICAM-1 mRNA expression (4.5 folds and 3 folds respectively (p < 0.05 vs untreated) was also observed after EMV pg (30 nM) stimulation whereas P. gingivalis infection was less effective, suggesting a specific triggering by EMV pg. Kinasome analysis demonstrated the specific effect induced by EMV pg on main pro-inflammatory pathways including JNK/AKT and STAT. EMV pg are effective pro-inflammatory effectors that may have detrimental effect on vascular homeostasis and should be considered as potential autocrine and paracrine effectors involved in the link between periodontitis and atherothrombosis. Periodontitis is an infectious inflammatory disease associated with soft tissue inflammation and destruction of the tooth-supporting tissues characterized by increased periodontal pocket depth, gingival bleeding and clinical attachment loss. Such disease impairs oral health related quality of life and is considered the main cause of dental mobility and tooth loss 1. Periodontitis affects a large proportion of the worldwide population and prevalence of its severe forms is estimated to be around 11% with a peak incidence around 38 years of age 2. The development of periodontitis is associated with the establishment of a dysbiosis characterized by the predominance of anaerobic species, including Porphyromonas gingivalis (P. gingivalis), and an imbalanced host-immune response inducing periodontal tissue destruction 3. During the last decades, periodontal diseases were associated with various chronic diseases such as diabetes, rheumatoid arthritis, adverse pregnancy outcomes and cardiovascular diseases, suggesting a systemic impact 4-6 with an enhanced proportion of worsened cardiovascular outcomes 7-9. Indeed, patients with periodontitis are more prone to endothelial dysfunction, aneurysmal disease progression 10 , coronary artery narrowing, and an increased all-cause and cardio-vascular related mortality 11,12. Moreover, in a clinical trial, intensive periodontal

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Proteomic analysis of the antimicrobial effects of sublethal concentrations of thymol on Salmonella enterica serovar Typhimurium

Zhao

Wang

Pei

et al. 2020

Appl Microbiol Biotechnol

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Salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence

Cited by 26 publications

References 37 publications

Transcriptional Profiling and Molecular Characterization of the yccT Mutant Link: A Novel STY1099 Protein with the Peroxide Stress Response and Cell Division of Salmonella enterica Serovar Enteritidis

Transcriptional Profiling and Molecular Characterization of the yccT Mutant Link: A Novel STY1099 Protein with the Peroxide Stress Response and Cell Division of Salmonella enterica Serovar Enteritidis

Porphyromonas gingivalis triggers the shedding of inflammatory endothelial microvesicles that act as autocrine effectors of endothelial dysfunction

Proteomic analysis of the antimicrobial effects of sublethal concentrations of thymol on Salmonella enterica serovar Typhimurium

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