Salmonella Harborage Sites in Infected Poultry That May Contribute to Contamination of Ground Meat

Rimet, Claire-Sophie; Maurer, John J.; Pickler, Larissa; Stabler, Lisa; Johnson, Kasey K.; Berghaus, Roy D.; Villegas, Ana; Lee, Margie; França, Monique

doi:10.3389/fsufs.2019.00002

Cited by 10 publications

(10 citation statements)

References 31 publications

(43 reference statements)

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“…The lux operon expressed under the sig70c35 promoter was strong enough to facilitate image acquisition at low exposure. In their study, Rimet et al ( 22 ) used a long exposure time (3–5 min) for image acquisition optimized to the lux operon expressed under the EM7 promoter, which has been described by Burns-Guydish et al ( 23 ). The EM7 promoter contains the sequences TTGACA in the “-35 region” (included in sig70c35) and TATAAT in the “-10 region” (not included in sig70c35), which are recognition consensus sequences for sigma factor family proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Hence it is unknown if the expression of the lux operon from two different sites in the chromosome has an impact on signal strength and Salmonella performance in the chicken milieu in vivo . Long exposure time will be necessary as described by Rimet et al ( 22 ) to image older birds (42 days old) as the tissues become less transparent. Longer exposure time using the reporter sig70c35 lux has not been conducted in this study but will be an option to improve the sensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…(Figure 8). In a series of experiments conducted by Rimet et al (22), it was estimated that the limit of detection of Salmonella strains (S. Typhimurium and S. Heidelberg) from the chicken cecum was >10 6 CFU/g for reliable visualization. Hence, our estimated detection limits are in line with these findings.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

et al. 2022

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The light emitting module lux operon (luxCDABE) of Photorhabdus luminescens can be integrated into a “dark” bacterium for expression under a suitable promoter. The technique has been used to monitor kinetics of infection, e.g., by studying gene expression in Salmonella using mouse models in vivo and ex vivo. Here, we applied the bioluminescence imaging (BLI) technique to track Salmonella Enteritidis (SEn) strains carrying the lux operon expressed under a constitutive promoter sequence (sigma 70) in chicken after oral challenge. Detectable photon signals were localized in the crop, small intestine, cecum, and yolk sac in orally gavaged birds. The level of colonization was determined by quantification of signal intensity and SEn prevalence in the cecum and yolk sac. Furthermore, an isogenic SEn mutant strain tagged with the lux operon allowed for us to assess virulence determinants regarding their role in colonization of the cecum and yolk sac. Interestingly, mutations of SPI-1(Salmonella Pathogenicity Island 1) and fur (ferric uptake regulator) showed significantly decreased colonization in yolk sac that was correlated with the BLI data. A similar trend was detected in a ΔtonB strain by analyzing enrichment culture data. The inherently low quantum yield, light scattering, and absorption by tissues did not facilitate detection of signals from live birds. However, the detection limit of lux operon has the potential to be improved by resonance energy transfer to a secondary molecule. As a proof-of-concept, we were able to show that sensitization of a fluorescent-bound molecule known as the lumazine protein (LumP) improved the limit of detection to a certain extent.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

et al. 2022

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“…For chicken cut drumsticks, which are bone-in products, a previous study suggested that internalized Salmonella through bone could be the cause of disease in these products. [27]. Almost all detection methods are consistent, including LAMP-LFD, LAMP-AGE, PCR, and the culture method.…”

Section: A C C E P T E Dmentioning

confidence: 89%

“…LAMP products have been detected using a variety of approaches. The lateral flow dipstick (LFD) assay provides good specificity testing and visual result evaluation[27]. In the previous study, the researchers developed the LAMP technique in conjunction with the LFD technique to examine and report the results.…”

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Development of a lateral flow dipstick test for the detection of 4 strains of Salmonella spp. in animal products and animal production environmental samples based on loop-mediated isothermal amplification

2023

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Development of a lateral flow dipstick test for the detection of 4 strains of Salmonella spp. in animal products and animal production environmental samples based on loopmediated isothermal amplification ABSTRACT Objective: This study aimed to develop loop-mediated isothermal amplification (LAMP) combined with lateral flow dipstick (LFD) to and compare it with LAMP-AGE, PCR, and standard Salmonella culture as reference methods for detecting Salmonella contamination in animal products and animal production environmental samples.Methods: The SalInvA01 primer, derived from the InvA gene and designed as a new probe for LFD detection, was used in developing this study. Adjusting for optimal conditions by temperature, time, and reagent concentration includes evaluating the specificity and limit of detection. The sampling of 120 animal product samples and 350 animal production environmental samples was determined by LAMP-LFD, comparing LAMP-AGE, PCR, and the culture method.Results: Salmonella was amplified using optimal conditions for the LAMP reaction and a DNA probe for LFD at 63°C for 60 minutes. The specificity test revealed no cross-reactivity with other microorganisms. Conclusion:The LAMP-LFD Salmonella detection assay, which used a specific region on the InvA gene, was highly specific, sensitive, and convenient for identifying Salmonella. Furthermore, this method could be used for Salmonella monitoring and primary screening in animal products and animal production environmental samples.

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Evaluation of a modified live Salmonella typhimurium vaccination efficacy against Salmonella enterica serovar Infantis in broiler chickens at processing age

Jones¹,

Costa

Hofacre³

et al. 2021

Journal of Applied Poultry Research

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Salmonella Harborage Sites in Infected Poultry That May Contribute to Contamination of Ground Meat

Cited by 10 publications

References 31 publications

Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

Development of a lateral flow dipstick test for the detection of 4 strains of Salmonella spp. in animal products and animal production environmental samples based on loop-mediated isothermal amplification

Evaluation of a modified live Salmonella typhimurium vaccination efficacy against Salmonella enterica serovar Infantis in broiler chickens at processing age

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